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- Table of Contents
VAV3 · Western blot design guide
VAV3 Western Blot Planning Guide
Plan a VAV3 Western blot around the catalog-observed 97.8 kDa band, image-backed A03204-3 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
VAV3 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 97.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Adipose tissue | |
| Negative control | Heart muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 97.8 kDa |
| ⓘ | Localization | Not reported |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated VAV3 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adipose tissue |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A03204-3 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected VAV3 Western Blot Band Size?
Use the product-observed 97.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 97.8 kDa | Matches the authoritative product WB observation. |
| 97.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected VAV3 appearancePlan around 97.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 97.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 97.8 kDa from UniProt Q9UKW4; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A03204-3. |
| Situation | Likely cause | Next action |
|---|---|---|
| 97.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing VAV3 band
A03204-3 · VAV3
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for VAV3 Western blot
🧪Use Adipose tissue as the first positive-control candidate and Heart muscle as the HPA Not detected negative candidate.
Positive control: Adipose tissue (Medium)
Negative control: Heart muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for VAV3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced VAV3 Western Blot Tips
Deeper troubleshooting and optimisation questions for VAV3, answered from its protein features.
Which band should guide the blot?
Use 97.8 kDa, the observation attached to the authoritative A03204-3 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 97.8 kDa expectation.
Which positive control should I start with?
Start with Adipose tissue, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Heart muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for VAV3 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A03204-3 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
VAV3 Western Blot Reagents
Human-reactive VAV3 Western blot reagents with authoritative product imagery.
Real WB data
Anti-VAV3 Antibody Picoband®
Cat # A03204-3
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q9UKW4
- Human Protein Atlas — VAV3
- A03204-3 product record
- PMC4192823 — Vav3, a GEF for RhoA, Plays a Critical Role under High Glucose Conditions (Endocrinology and metabolism (Seoul, Korea), 2014)
- PMC2430719 — Vav3 oncogene activates estrogen receptor and its overexpression may be involved in human breast cancer (BMC cancer, 2008)
- PMC11008271 — The role of STAT3/VAV3 in glucolipid metabolism during the development of HFD-induced MAFLD (International journal of biological sciences, 2024)
