WIPI2 Antibodies

AND WIPI2 ELISA kits, WIPI2 Proteins

Many biological assays use antibodies to detect WIPI2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with WIPI2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop WIPI2 antibodies...

We validate the specificity of these antibodies to WIPI2 by testing them on tissues known to express WIPI2 positively and negatively. Browse below to find the WIPI2 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for WIPI2? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the WIPI2 Western Blot Guide

WIPI2 Infographic by Boster Bio

All WIPI2 Products

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Product

Figures

Specificity

Applications

Price

WIPI2 (NM_015610) Human Over-expression Lysate

WIPI2 Human Over-expression Lysates NM_001033519

Human

$628
Cat # LS026100
20 µg, 100 µg

Anti-WIPI2 Antibody

Western blot analysis of extracts of various cell lines, using WIPI2 antibody at 1:1000 dilution

Western blot analysis of extracts of Rat testis, using WIPI2 antibody at 1:1000 dilution

Human, Mouse, Rat

$405
Cat # A06492-1
100ul

Anti-WIPI2 Antibody

Western blot analysis of WIPI2 in human testis tissue lysate with WIPI2 antibody at 1 μg/mL

Human, Rat

ELISA, WB

$481
Cat # A06492
0.1 mg

WIPI2 Overview

Facts about WD repeat domain phosphoinositide-interacting protein 2.

WIPI2 3D structure. Source: alphafold

WD repeat domain phosphoinositide-interacting protein 2 (WIPI2)

Early component of the autophagy machinery being involved in creation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.

Gene Information

Human
Gene Name:	WIPI2
Uniprot:	Q9Y4P8
Entrez:	26100

Family

Belongs to:

WD repeat SVP1 family

Alternative Names

ATG18B; Atg21; CGI-50; DKFZP434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI-2; WIPI49-like protein 2

Molecular Weight

Mass (kDA):
49.408 kDA

Genomic Context


Human

Location:	7p22.1
Sequence:	7; NC_000007.14 (5190233..5233855)

Tissue Specificity

Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.

Subcellular Localizations

Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side. Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.

Diseases

Malignant Neoplasms
Foot-and-mouth Disease

Interacting Proteins

ARL6IP1
POT1
RABAC1

Pathways

Autophagy
Cell Migration
Catabolic Process

PTMs

Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/WIPI2

References

PMID: 15602573 by Proikas-Cezanne T., et al. WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy.

PMID: 20505359 by Polson H.E., et al. Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.