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Product Info Summary
| SKU: | A06492 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-WIPI2 Antibody
SKU/Catalog Number
A06492
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WIPI2 Antibody (Catalog # A06492). Tested in ELISA, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
WIPI2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-WIPI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06492)
Host
Rabbit
Contents
WIPI2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal WIPI2 antibody was raised against a 17 amino acid peptide near the amino terminus of human WIPI2. The immunogen is located within the first 50 amino acids of WIPI2.
Cross-reactivity
WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1.
Reactive Species
A06492 is reactive to WIPI2 in Human, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
49.4 kDa
Background of WIPI2
WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06492 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WIPI2 in human testis tissue lysate with WIPI2 antibody at 1 μg/mL
Specific Publications For Anti-WIPI2 Antibody (A06492)
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