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Product Info Summary
|Reactive Species:||Human, Rat|
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Boster Bio Anti-WIPI2 Antibody (Catalog # A06492). Tested in ELISA, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
WIPI2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-WIPI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06492)
WIPI2 Antibody is supplied in PBS containing 0.02% sodium azide.
Rabbit polyclonal WIPI2 antibody was raised against a 17 amino acid peptide near the amino terminus of human WIPI2. The immunogen is located within the first 50 amino acids of WIPI2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1.
A06492 is reactive to WIPI2 in Human, Rat
A06492 is guaranteed for ELISA, WB Boster Guarantee
Background of WIPI2
WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μg/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
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Western blot analysis of WIPI2 in human testis tissue lysate with WIPI2 antibody at 1 μg/mL
Gene/Protein Information For WIPI2 (Source: Uniprot.org, NCBI)
WD repeat domain phosphoinositide-interacting protein 2
WD repeat SVP1 family
ATG18B; Atg21; CGI-50; DKFZP434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI-2; WIPI49-like protein 2 WIPI2 ATG18B, Atg21, CGI-50, IDDSSA, WIPI-2 WD repeat domain, phosphoinositide interacting 2 WD repeat domain phosphoinositide-interacting protein 2|WD40 repeat protein interacting with phosphoinositides 2|WIPI49-like protein 2*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on WIPI2, check out the WIPI2 Infographic
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In this infographic, you will see the following information for WIPI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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