Bovine IL-6 ELISA Kit PicoKine®

Product Name

Bovine IL-6 ELISA Kit PicoKine®

SKU/Catalog Number

EK2391

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Bovine IL-6 ELISA Kit PicoKine® (96 Tests). Quantitate Bovine IL-6 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA or citrate). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Bovine IL-6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2391)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: Pichia pastori; Immunogen sequence: G30-K208

Capture/Detection Antibodies

The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

See datasheet of EK2391 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2391 is reactive to IL6 in Bovine samples

Validated Sample Types

cell culture supernatants, cell lysates, serum, plasma(heparin, EDTA or citrate).

Application Guarantee

EK2391 is guaranteed for ELISA in Bovine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL6

The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) (interleukin-6), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

Kit Components

Catalog Number	Description	Quantity
EK2391-CAP	Anti-Bovine IL6 Pre-coated 96-well strip microplate	1
EK2391-ST	Bovine IL6 Standard	2 vials, 10 ng/tube
EK2391-DA	Bovine IL6 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.008	0.078	0.151	0.294	0.536	1.015	1.555	2.055

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Bovine IL-6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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