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Product Info Summary
|Description:||Transient overexpression lysate of BRCA1 interacting protein C-terminal helicase 1 (BRIP1)|
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BRIP1 (NM_032043) Human Over-expression Lysate
Transient overexpression lysate of BRCA1 interacting protein C-terminal helicase 1 (BRIP1)
Storage & Handling
Cite This Product
BRIP1 (NM_032043) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083990)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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BRIP1 Human Over-expression Lysates NM_032043.
Western blot validation of overexpression lysate (LS083990) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BRIP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for BRIP1 (Source: Uniprot.org, NCBI)
Fanconi anemia group J protein
DEAD box helicase family
ATP-dependent RNA helicase BRIP1; BACH1; BACH1BRCA1/BRCA2-associated helicase 1; BRCA1 interacting protein C-terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRIP1; EC 3.6.1; EC 188.8.131.52; FACJ; FANCJ; FANCJFanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ BRIP1 BACH1, FANCJ, OF BRCA1 interacting helicase 1 Fanconi anemia group J protein|ATP-dependent RNA helicase BRIP1|BRCA1 interacting protein C-terminal helicase 1|BRCA1-associated C-terminal helicase 1|BRCA1-binding helicase-like protein BACH1|BRCA1/BRCA2-associated helicase 1|FANCJ helicase*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on BRIP1, check out the BRIP1 Infographic
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In this infographic, you will see the following information for BRIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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