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Product Info Summary
| SKU: | LS000699 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
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Product info
Product Name
BUB1 (NM_004336) Human Over-expression Lysate
SKU/Catalog Number
LS000699
Size
100 µg
Description
Transient overexpression lysate of budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1)
Storage & Handling
Cite This Product
BUB1 (NM_004336) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000699)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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BUB1 Human Over-expression Lysates NM_004336.
Western blot validation of overexpression lysate (LS000699) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BUB1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for BUB1 (Source: Uniprot.org, NCBI)
Gene Name
BUB1
Full Name
Mitotic checkpoint serine/threonine-protein kinase BUB1
Weight
122.375kDa
Superfamily
protein kinase superfamily
Alternative Names
BUB1 budding uninhibited by benzimidazoles 1 homolog; Bub1; BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1; BUB1L; budding uninhibited by benzimidazoles 1 (yeast homolog); budding uninhibited by benzimidazoles 1 homolog (yeast); EC 2.7.11.1; hBUB1mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase BUB1 BUB1AL, hBUB1, BUB1 BUB1 mitotic checkpoint serine/threonine kinase mitotic checkpoint serine/threonine-protein kinase BUB1|BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1 homolog|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on BUB1, check out the BUB1 Infographic
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In this infographic, you will see the following information for BUB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For BUB1 (NM_004336) Human Over-expression Lysate (LS000699)
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