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Product Info Summary
|Description:||Transient overexpression lysate of integrin alpha FG-GAP repeat containing 3 (ITFG3)|
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C16orf9 (FAM234A) (NM_032039) Human Over-expression Lysate
Transient overexpression lysate of integrin alpha FG-GAP repeat containing 3 (ITFG3)
Storage & Handling
Cite This Product
C16orf9 (FAM234A) (NM_032039) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083986)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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C16orf9 (FAM234A) Human Over-expression Lysates NM_032039.
Western blot validation of overexpression lysate (LS083986) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM234A (Myc-DDK-tagged) Human family with sequence similarity 234 member A (FAM234A)
Gene/Protein Information for FAM234A (Source: Uniprot.org, NCBI)
Protein FAM234A FAM234A C16orf9, ITFG3, gs19 family with sequence similarity 234 member A protein FAM234A|integrin alpha FG-GAP repeat containing 3|protein ITFG3*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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