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Product Info Summary
|Description:||Transient overexpression lysate of chromosome 1 open reading frame 124 (C1orf124), transcript variant 1|
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C1orf124 (SPRTN) (NM_032018) Human Over-expression Lysate
Transient overexpression lysate of chromosome 1 open reading frame 124 (C1orf124), transcript variant 1
Storage & Handling
Cite This Product
C1orf124 (SPRTN) (NM_032018) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083932)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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C1orf124 (SPRTN) Human Over-expression Lysates NM_032018.
Western blot validation of overexpression lysate (LS083932) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SPRTN (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SPRTN (Source: Uniprot.org, NCBI)
SprT-like domain-containing protein Spartan
C1orf124; chromosome 1 open reading frame 124; DDDL1880; dJ876B10.3; DKFZP547N043; PRO4323; RP5-876B10.3; Spartan; SprT-like N-terminal domain; zinc finger RAD18 domain-containing protein C1orf124 SPRTN C1orf124, DVC1, PRO4323, spartan SprT-like N-terminal domain DNA-dependent metalloprotease SPRTN|DNA damage protein targeting VCP|DNA damage-targeting VCP (p97) adaptor|zinc finger RAD18 domain-containing protein*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS083932
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