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Product Info Summary
|Description:||Transient overexpression lysate of chromosome 22 open reading frame 23 (C22orf23)|
C22orf23 (NM_032561) Human Over-expression Lysate
Transient overexpression lysate of chromosome 22 open reading frame 23 (C22orf23)
Storage & Handling
Cite This Product
C22orf23 (NM_032561) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084645)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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C22orf23 Human Over-expression Lysates NM_032561.
Western blot validation of overexpression lysate (LS084645) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with C22orf23 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for C22orf23 (Source: Uniprot.org, NCBI)
UPF0193 protein EVG1
UPF0193 (EVG1) family
chromosome 22 open reading frame 23; dJ1039K5.6; FLJ32787 C22orf23 EVG1, dJ1039K5.6 chromosome 22 open reading frame 23 UPF0193 protein EVG1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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