CALB1 Calbindin-1 Rat Recombinant Protein

Product Name

CALB1 Calbindin-1 Rat Recombinant Protein

View all Calbindin D-28K recombinant proteins

SKU/Catalog Number

PROTP07171

Size

1ug, 5ug, 100ug

Description

Recombinant Rat Calbindin-1 produced in E. coli. The Rat CALB1 is purified by proprietary chromatographic techniques. Applications: CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption, we suggest the following procedure:
A- Dilute 1 µl of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).
B- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.

Storage & Handling

Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP28K should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

CALB1 Calbindin-1 Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP07171)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated solution (1mg/ml) containing 50mM NaHCO₃.

Purity

Greater than 90% as determined by SDS-PAGE.

Predicted MW

30.025kDa

Reconstitution

It is recommended to reconstitute the lyophilized CABP28K in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

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Reconstitution

It is recommended to reconstitute the lyophilized CABP28K in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For CALB1 (Source: Uniprot.org, NCBI)

Alternative Names

CAB27; CALB; calbindin 1, (28kD); calbindin 1, 28kDa; Calbindin D28; calbindin; D-28K; RTVL-H protein; Vitamin D-dependent calcium-binding protein, avian-type CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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