|Validated Species:||Bovine, Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Calbindin-D Antibody (Monoclonal, CB-955)|
|Description||Mouse IgG monoclonal antibody for Calbindin-D, calbindin 1, 28kDa (CALB1) detection. Tested with WB, IHC-P in Human;mouse;rat;bovine. No cross reactivity with other proteins.|
|Cite This Product||Anti-Calbindin-D Antibody (Monoclonal, CB-955) CB-955 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1007)|
|Replacement Item||This antibody may replace the following items: sc-135666|sc-28285|sc-365360|sc-7691|sc-7692 from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Bovine kidney calbindin-D|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||29994 MW|
|Protein Function||Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.|
|Sequence Similarities||Belongs to the calbindin family.|
|Alternative Names||Calbindin;Calbindin D28;D-28K;Spot 35 protein;Vitamin D-dependent calcium-binding protein, avian-type;Calb1;|
|Research Areas|||neuroscience|neurotransmission|calcium signaling|calcium binding proteins| neuroscience|cell type marker|neuron marker|soma marker||
Background for Calbindin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Calbindin-D Antibody (Monoclonal, CB-955) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, bovine, mouse, rat, By Heat
Western blot, 0.5-1μg/ml, Human, bovine, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Calbindin-D Antibody (Monoclonal, CB-955) Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
IHC(P): Mouse Kidney Tissue
IHC(P): Rat Kidney Tissue
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Mammary Cancer Tissue