radio
SKU MA1007
Size 100μg/vial
Reactivity Bovine, Human, Mouse, Rat
Clonality Monoclonal
Clone Number CB-955
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Calbindin-D Antibody (Monoclonal, CB-955)
SKU/Catalog Number MA1007
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Calbindin-D, calbindin 1, 28kDa (CALB1) detection. Tested with WB, IHC-P in Human;mouse;rat;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Calbindin-D Antibody (Monoclonal, CB-955) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1007)
Host Mouse
Immunogen Bovine kidney calbindin-D
Reactivity Bovine, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, bovine, mouse, rat, By Heat
Western blot, 0.5-1μg/ml, Human, bovine, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1007 1 WB anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate

/antibody/ma1007 2 IHC anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Mouse Kidney Tissue

/antibody/ma1007 3 IHC anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Rat Kidney Tissue

/antibody/ma1007 4 IHC anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/antibody/ma1007 5 IHC anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P07171
Gene Name Calb1
Protein Name Calbindin
Alternative Names Calbindin;Calbindin D28;D-28K;Spot 35 protein;Vitamin D-dependent calcium-binding protein, avian-type;Calb1;
Molecular Weight 29994 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Background The human calbindin D28k(CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al.(1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.

Order Product (MA1007)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.