Anti-Calbindin Antibody

Boster Bio Anti-Calbindin Antibody catalog # M03047-2. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat. Independently reviewed in 1 review(s).

Product Info Summary

SKU: M03047-2
Size: 100ul
Reactive Species: Bovine, Human, Mouse, Rat
Host: Chicken
Application: IF, IHC, WB

Product Name

Anti-Calbindin Antibody

See all Calbindin D-28K products

SKU/Catalog Number

M03047-2

Size

100ul

Description

Boster Bio Anti-Calbindin Antibody catalog # M03047-2. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calbindin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03047-2)

Host

Chicken

Contents

Supplied as an aliquot of IgY preparation plus 5mM NaN3

Clonality

Polyclonal

Immunogen

Full-length recombinant human protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03047-2 is reactive to CALB1 in Bovine, Human, Mouse, Rat

Applications

M03047-2 is guaranteed for IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:5,000. IF/ICC or IHC: 1:1,000-1:5,000.

Validation Images & Assay Conditions

Gene/Protein Information For CALB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05937

Gene ID

793

Gene Name

CALB1

Full Name

Calbindin

Weight

30.025kDa

Superfamily

calbindin family

Alternative Names

CAB27; CALB; calbindin 1, (28kD); calbindin 1, 28kDa; Calbindin D28; calbindin; D-28K; RTVL-H protein; Vitamin D-dependent calcium-binding protein, avian-type CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CALB1, check out the CALB1 Infographic

CALB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CALB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M03047-2

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Calbindin Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

1 Reviews For Anti-Calbindin Antibody

0

"Best Calbindin Antibody for IHC in Mouse Cerebellum"

Excellent

Source: Biocompare.com

"Best Calbindin Antibody for IHC in Mouse Cerebellum"

--Dinesh Joshi, University of Wisconsin Madison, Research Scientist

Application Immunohistochemistry
Sample Mice Cerebellum
Detection Fluorescence

"I tried this antibody for Purkinje cell labeling in mice cerebellum using IHC-P. Antigen retrieval - 20min boiling in EDTA based buffer. Permeabilization - 0.3% triton for 15min. Blocking - 10% goat serum for 1hr. Primary antibody - 1:250 overnight at 4 Degree C. Secondary antibody - Fluorescence conjugated secondary antibody at 1:250 for 2hr at RT. I was amazed to see the beautiful dendrites, soma and axons. In my hand, this is the best labeling of Purkinje cells I have ever seen using IHC-P. Beautiful labeling of Purkinje cell soma, dendrites and axons. Best antibody I have used till now. Highly recommended for IHC-P."

Verified customer

Submitted

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-Calbindin Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney lung using anti-Calbindin antibody M03047-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-29

Question

We want to test anti-Calbindin antibody M03047-2 on bovine kidney lung for research purposes, then I may be interested in using anti-Calbindin antibody M03047-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Jackson

Verified customer

Asked: 2019-11-28

Answer

The products we sell, including anti-Calbindin antibody M03047-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-28

Question

Will M03047-2 anti-Calbindin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

As indicated on the product datasheet, M03047-2 anti-Calbindin antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-14

Question

See below the WB image, lot number and protocol we used for kidney lung using anti-Calbindin antibody M03047-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-08

Question

We have seen staining in human kidney lung. What should we do? Is anti-Calbindin antibody supposed to stain kidney lung positively?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

Based on literature kidney lung does express CALB1. Based on Uniprot.org, CALB1 is expressed in caudate nucleus, brain, amygdala, cerebellum substantia nigra, kidney lung, among other tissues. Regarding which tissues have CALB1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cerebellum, and Substantia nigra, Pubmed ID: 14702039
Brain, Pubmed ID: 2565876, 3691519
Kidney, and Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-09-27

Question

I was wanting to use your anti-Calbindin antibody for IF for bovine kidney lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for bovine kidney lung identification?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

It shows on the product datasheet, M03047-2 anti-Calbindin antibody has been validated for IF, IHC, WB on bovine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in bovine kidney lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-23

Question

Is there a BSA free version of anti-Calbindin antibody M03047-2 available?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Calbindin antibody M03047-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-06

Question

you antibody using your anti-Calbindin antibody for retina layer formation studies. Has this antibody been tested with western blotting on neuronal cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

We appreciate your inquiry. This M03047-2 anti-Calbindin antibody is validated on neuronal cells. It is guaranteed to work for IF, IHC, WB in bovine, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-03

Question

I see that the anti-Calbindin antibody M03047-2 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-01-21

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-01-21

Question

Is this M03047-2 anti-Calbindin antibody reactive to the isotypes of CALB1?

Verified Customer

Verified customer

Asked: 2018-11-30

Answer

The immunogen of M03047-2 anti-Calbindin antibody is Full-length recombinant human protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-11-30

Question

I have a question about product M03047-2, anti-Calbindin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03047-2 anti-Calbindin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-12

Question

Is a blocking peptide available for product anti-Calbindin antibody (M03047-2)?

Verified Customer

Verified customer

Asked: 2017-10-20

Answer

We do provide the blocking peptide for product anti-Calbindin antibody (M03047-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-20

Question

We are currently using anti-Calbindin antibody M03047-2 for bovine tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says bovine, human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-28

Answer

The anti-Calbindin antibody (M03047-2) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-28

Question

Does anti-Calbindin antibody M03047-2 work for IF with kidney lung?

S. Rodriguez

Verified customer

Asked: 2016-02-18

Answer

According to the expression profile of kidney lung, CALB1 is highly expressed in kidney lung. So, it is likely that anti-Calbindin antibody M03047-2 will work for IF with kidney lung.

Boster Scientific Support

Answered: 2016-02-18

Question

We ordered your anti-Calbindin antibody for IF on brain a few years ago. I am using bovine, and We intend to use the antibody for WB next. I am interested in examining brain as well as amygdala in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

J. Jones

Verified customer

Asked: 2013-12-23

Answer

I looked at the website and datasheets of our anti-Calbindin antibody and I see that M03047-2 has been validated on bovine in both IF and WB. Thus M03047-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-12-23

$299

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M03047-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.