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Product Info Summary
|Description:||Transient overexpression lysate of small optic lobes homolog (Drosophila) (SOLH)|
Calpain 15 (CAPN15) (NM_005632) Human Over-expression Lysate
Transient overexpression lysate of small optic lobes homolog (Drosophila) (SOLH)
Storage & Handling
Cite This Product
Calpain 15 (CAPN15) (NM_005632) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006650)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Calpain 15 (CAPN15) Human Over-expression Lysates NM_005632.
Western blot validation of overexpression lysate (LS006650) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CAPN15 (Myc-DDK-tagged) Human calpain 15 (CAPN15) using transfection reagent.
Gene/Protein Information for CAPN15 (Source: Uniprot.org, NCBI)
peptidase C2 family
Calpain-15 CAPN15 SOLH calpain 15 calpain-15|small optic lobes homolog*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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