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Product Info Summary
| SKU: | EK2331 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Canine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA) |
Product info
Product Name
Canine CCL2/MCP-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2331
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Canine CCL2/MCP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Canine CCL2/MCP-1 in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Canine CCL2/MCP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2331)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Gln24-Pro101
Sensitivity
<2 pg/mL
Assay Range
15.6-1,000 pg/mL
Standard Dilution Instructions
See datasheet of EK2331 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2331 is reactive to CCL2 in Canine samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA)
Application Guarantee
EK2331 is guaranteed for ELISA in Canine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL2
Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2331-CAP | Anti-Canine CCL2 Pre-coated 96-well strip microplate | 1 |
| EK2331-ST | Canine CCL2 Standard | 2 vials, 10 ng/tube |
| EK2331-DA | Canine CCL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.006 | 0.097 | 0.199 | 0.372 | 0.721 | 1.278 | 1.863 | 2.361 |
Data Example Images
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Canine CCL2/MCP-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Canine CCL2/MCP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 28 | 123 | 319 | 31 | 119 | 314 |
| Standard deviation | 2.00 | 9.27 | 13.40 | 1.90 | 4.94 | 11.74 |
| CV (%) | 7.2% | 7.5% | 4.2% | 6.1% | 4.2% | 3.7% |
Specific Publications For Canine CCL2/MCP-1 ELISA Kit PicoKine® (EK2331)
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