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Product Info Summary
| SKU: | EK2344 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Canine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA) |
Product info
Product Name
Canine CCL5/RANTES ELISA Kit PicoKine®
SKU/Catalog Number
EK2344
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Canine CCL5/RANTES ELISA Kit PicoKine® (96 Tests). Quantitate Canine CCL5/RANTES in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Canine CCL5/RANTES ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2344)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Ser24-Ser91
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<5 pg/mL
Assay Range
23.4-1,500 pg/mL
Standard Dilution Instructions
See datasheet of EK2344 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2344 is reactive to CCL5 in Canine samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA)
Application Guarantee
EK2344 is guaranteed for ELISA in Canine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL5
Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene. It is also known as RANTES. This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2344-CAP | Anti-Canine CCL5 Pre-coated 96-well strip microplate | 1 |
| EK2344-ST | Canine CCL5 Standard | 2 vials, 10 ng/tube |
| EK2344-DA | Canine CCL5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 23.4 | 46.8 | 93.7 | 187.5 | 375 | 750 | 1500 |
| O.D. | 0.03 | 0.14 | 0.242 | 0.424 | 0.77 | 1.165 | 1.678 | 2.15 |
Data Example Images
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Canine CCL5/RANTES PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Canine CCL5/RANTES ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 36 | 174 | 674 | 31 | 125 | 716 |
| Standard deviation | 1.97 | 7.85 | 34.31 | 1.93 | 9.13 | 14.68 |
| CV (%) | 5.5% | 4.5% | 5.1% | 6.2% | 7.3% | 2.1% |
Specific Publications For Canine CCL5/RANTES ELISA Kit PicoKine® (EK2344)
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