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Product Info Summary
| SKU: | LS000966 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 1 |
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Product info
Product Name
CD59 (NM_203330) Human Over-expression Lysate
SKU/Catalog Number
LS000966
Size
100 µg
Description
Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
CD59 (NM_203330) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000966)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CD59 Human Over-expression Lysates NM_000611.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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CD59 Human Over-expression Lysates NM_001127223.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
CD59 Human Over-expression Lysates NM_001127225.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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CD59 Human Over-expression Lysates NM_001127226.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
CD59 Human Over-expression Lysates NM_001127227.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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CD59 Human Over-expression Lysates NM_203329.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
CD59 Human Over-expression Lysates NM_203329.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
CD59 Human Over-expression Lysates NM_203330.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
CD59 Human Over-expression Lysates NM_203330.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
CD59 Human Over-expression Lysates NM_203331.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
CD59 Human Over-expression Lysates NM_203331.
Western blot validation of overexpression lysate (LS000966) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CD59 (Source: Uniprot.org, NCBI)
Gene Name
CD59
Full Name
CD59 glycoprotein
Weight
14.177kDa
Alternative Names
16.3A5; 1F5 antigen; 1F5; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16; CD59 antigen; CD59 antigen, complement regulatory protein; CD59 glycoprotein; CD59 molecule, complement regulatory protein; CD59; EJ16; EJ30; EJ30, EL32 and G344); EL32; FLJ38134; FLJ92039; G344; HRF20; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MACIF; MAC-inhibitory protein; MAC-IP; MEM43 antigen; MEM43; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane in CD59 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20 CD59 molecule (CD59 blood group) CD59 glycoprotein|1F5 |20 kDa homologous restriction factor|CD59 p18-20 ( by antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 blood group |CD59 molecule, complement regulatory protein|Ly-6-like protein|MEM43 |T cell-activating protein|human leukocyte MIC11|lymphocytic CD59/MEM43|membrane attack complex (MAC) inhibition factor|membrane attack complex inhibition factor|membrane inhibitor of reactive lysis|protectin|surface anitgen recognized by 16.3A5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CD59, check out the CD59 Infographic
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In this infographic, you will see the following information for CD59: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CD59 (NM_203330) Human Over-expression Lysate (LS000966)
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