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Product Info Summary
|Description:||Transient overexpression lysate of centrosomal protein 76kDa (CEP76)|
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CEP76 (NM_024899) Human Over-expression Lysate
Transient overexpression lysate of centrosomal protein 76kDa (CEP76)
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Cite This Product
CEP76 (NM_024899) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS079959)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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CEP76 Human Over-expression Lysates NM_024899.
Western blot validation of overexpression lysate (LS079959) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CEP76 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for CEP76 (Source: Uniprot.org, NCBI)
Centrosomal protein of 76 kDa
C18orf9; centrosomal protein 76kDa; centrosomal protein of 76 kDa; Cep76; chromosome 18 open reading frame 9; FLJ12542; HsT1705 CEP76 C18orf9, HsT1705 centrosomal protein 76 centrosomal protein of 76 kDa|centrosomal protein 76kDa*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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