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Product Info Summary
| SKU: | EKC1914 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
Cytochrome P450 2J2 Colorimetric Cell-Based ELISA
See all Cytochrome p450 2J2 products
SKU/Catalog Number
EKC1914
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The Cytochrome P450 2J2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cytochrome P450 2J2 protein expression profile in cells. The kit can be used for measuring the relative amounts of Cytochrome P450 2J2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cytochrome P450 2J2.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Cytochrome P450 2J2 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1914)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1914 is reactive to CYP2J2 in Human samples
Application Guarantee
EKC1914 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-CYP2J2 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from A549/COS7 cells
Protein Target Info & Infographic
Gene/Protein Information For CYP2J2 (Source: Uniprot.Org, NCBI)
Gene Name
CYP2J2
Full Name
Cytochrome P450 2J2
Weight
57.611kDa
Superfamily
cytochrome P450 family
Alternative Names
Arachidonic acid epoxygenase; CPJ2; CYPIIJ2; cytochrome P450 2J2; cytochrome P450, family 2, subfamily J, polypeptide 2; cytochrome P450, subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2; EC 1.14.14.1; flavoprotein-linked monooxygenase; microsomal monooxygenase CYP2J2 CPJ2, CYPIIJ2 cytochrome P450 family 2 subfamily J member 2 cytochrome P450 2J2|albendazole monooxygenase (hydroxylating)|albendazole monooxygenase (sulfoxide-forming)|arachidonic acid epoxygenase|cytochrome P450, family 2, subfamily J, polypeptide 2|cytochrome P450, subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2|flavoprotein-linked monooxygenase|hydroperoxy icosatetraenoate isomerase|microsomal monooxygenase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CYP2J2, check out the CYP2J2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CYP2J2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Cytochrome P450 2J2 Colorimetric Cell-Based ELISA (EKC1914)
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