Anti-MAPK6 Antibody Picoband™

ERK3/MAPK6 antibody

Boster Bio Anti-MAPK6 Antibody Picoband™ catalog # PB9666. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9666
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-MAPK6 Antibody Picoband™

View all ERK3/MAPK6 Antibodies

SKU/Catalog Number

PB9666

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MAPK6 Antibody Picoband™ catalog # PB9666. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAPK6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9666)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MAPK6 recombinant protein (Position: E520-N721). Human MAPK6 shares 86.2% and 82.8% amino acid (aa) sequence identity with mouse and rat MAPK6, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9666 is reactive to MAPK6 in Human, Mouse, Rat

Applications

PB9666 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

82.681kDa

Background of ERK3/MAPK6

Mitogen-activated protein kinase 6 is an enzyme that in humans is encoded by the MAPK6 gene. It is mapped to 15q21. The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblastsupon treatment with serum or phorbol esters.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For MAPK6 (Source: Uniprot.org, NCBI)

Gene Name

MAPK6

Full Name

Mitogen-activated protein kinase 6

Weight

82.681kDa

Superfamily

protein kinase superfamily

Alternative Names

DKFZp686F03189; EC 2.7.11; EC 2.7.11.24; ERK3; ERK-3; ERK3p97-MAPK; Extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; mitogen-activated protein kinase 6; p97MAPK; PRKM6; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6 MAPK6 ERK3, HsT17250, PRKM6, p97MAPK mitogen-activated protein kinase 6 mitogen-activated protein kinase 6|ERK-3|MAP kinase 6|MAPK 6|extracellular signal-regulated kinase 3|extracellular signal-regulated kinase, p97|p97-MAPK|protein kinase, mitogen-activated 5|protein kinase, mitogen-activated 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAPK6, check out the MAPK6 Infographic

MAPK6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAPK6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9666 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chumyam A. and Saengnil,K. 2021.Transient H2O2 induction by ClO2 fumigation alters Prx-Trx system and causes MAPK accumulation attenuating browning in harvested longan fruit.CMUJ. Nat. Sci. 20(1): e2021013.
Species: Longan fruit cv. Daw
PB9666 usage in article: APP:WB, SAMPLE:PERICARP, DILUTION:1:1000

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3 Customer Q&As for Anti-MAPK6 Antibody Picoband™

Question

My question regarding product PB9666, anti-MAPK6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Jha

Verified customer

Asked: 2019-10-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9666 anti-MAPK6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-23

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal skeletal muscle using anti-MAPK6 antibody PB9666. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-22

Question

We are currently using anti-MAPK6 antibody PB9666 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

The anti-MAPK6 antibody (PB9666) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-02

Order DetailsPrice
PB9666

100μg

$370
PB9666-10ug

10μg sample (liquid)

$99
PB9666-Biotin

100 μg Biotin conjugated

$570
PB9666-Cy3

100 μg Cy3 conjugated

$570
PB9666-Dylight488

100 μg Dylight488 conjugated

$570
PB9666-Dylight550

100 μg Dylight550 conjugated

$570
PB9666-Dylight594

100 μg Dylight594 conjugated

$570
PB9666-FITC

100 μg FITC conjugated

$570
PB9666-HRP

100 μg HRP conjugated

$570
PB9666-APC

100 μg APC conjugated

$670
PB9666-PE

100 μg PE conjugated

$670
PB9666-carrier-free

Carrier Free

$370

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PB9666
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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