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Product Info Summary
| SKU: | PA1729 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PAK6 Antibody Picoband®
SKU/Catalog Number
PA1729
BA3705 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PAK6 Antibody catalog # PA1729. Tested in Flow Cytometry, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAK6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1729)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1729 is reactive to PAK6 in Human, Mouse, Rat
Observed Molecular Weight
70-75 kDa
Calculated molecular weight
74.9 kDa
Background of PAK6
Serine/threonine-protein kinase PAK 6 (p21 Protein-activated Kinase 6) is an enzyme that in humans is encoded by the PAK6 gene. The PAK6 gene is mapped to chromosome 15q15. based on an alignment of the PAK6 sequence with the genomic sequence. This gene encodes a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1729 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human CACO-2 whole cell, rat brain tissue, mouse brain tissue
ICC/IF: CACO-2 cell
FCM: CACO-2 cell
Validation Images & Assay Conditions
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Western blot analysis of PAK6 using anti-PAK6 antibody (PA1729).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK6 antigen affinity purified polyclonal antibody (PA1729) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAK6 at approximately 70-75 kDa. The expected band size for PAK6 is at 75 kDa.
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IF analysis of PAK6 using anti-PAK6 antibody (PA1729) and anti-Tubulin Alpha antibody (M03989-3).
PAK6 was detected in immunocytochemical section of CACO-2 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAK6 Antibody (PA1729) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of CACO-2 cells using anti-PAK6 antibody (PA1729).
Overlay histogram showing CACO-2 cells stained with PA1729 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAK6 Antibody (PA1729, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-PAK6 Antibody Picoband® (PA1729)
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7 Customer Q&As for Anti-PAK6 Antibody Picoband®
Question
We are currently using anti-PAK6 antibody PA1729 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The anti-PAK6 antibody (PA1729) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-24
Question
Will anti-PAK6 antibody PA1729 work on canine WB with brain?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Our lab technicians have not tested anti-PAK6 antibody PA1729 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PAK6 antibody PA1729 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-11
Question
Is there a BSA free version of anti-PAK6 antibody PA1729 available?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PAK6 antibody PA1729 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-03
Question
Would anti-PAK6 antibody PA1729 work for WB with prefrontal cortex?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
According to the expression profile of prefrontal cortex, PAK6 is highly expressed in prefrontal cortex. So, it is likely that anti-PAK6 antibody PA1729 will work for WB with prefrontal cortex.
Boster Scientific Support
Answered: 2019-12-19
Question
I have a question about product PA1729, anti-PAK6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1729 anti-PAK6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-05
Question
See below the WB image, lot number and protocol we used for prefrontal cortex using anti-PAK6 antibody PA1729. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-23
Question
Is this PA1729 anti-PAK6 antibody reactive to the isotypes of PAK6?
S. Patel
Verified customer
Asked: 2015-11-11
Answer
The immunogen of PA1729 anti-PAK6 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6(16-32aa QNFQHRVHTSFDPKEGK), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-11
