Anti-PAK6 Antibody

PAK6 antibody

Boster Bio Anti-PAK6 Antibody catalog # PA1729. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1729
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-PAK6 Antibody

View all PAK6 Antibodies

SKU/Catalog Number

PA1729

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PAK6 Antibody catalog # PA1729. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PAK6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1729)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1729 is reactive to PAK6 in Human, Mouse, Rat

Applications

PA1729 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

74.869kDa

Background of PAK6

Serine/threonine-protein kinase PAK 6 (p21 Protein-activated Kinase 6) is an enzyme that in humans is encoded by the PAK6 gene. The PAK6 gene is mapped to chromosome 15q15. based on an alignment of the PAK6 sequence with the genomic sequence. This gene encodes a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For PAK6 (Source: Uniprot.org, NCBI)

Gene Name

PAK6

Full Name

Serine/threonine-protein kinase PAK 6

Weight

74.869kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A)-activated kinase 6; p21-activated kinase 6; p21-activated protein kinase 6; PAK-5; PAK5EC 2.7.11.1; PAK6; PAK-6; serine/threonine-protein kinase PAK 6 PAK6 PAK5 p21 (RAC1) activated kinase 6 serine/threonine-protein kinase PAK 6|p21 protein (Cdc42/Rac)-activated kinase 6|p21(CDKN1A)-activated kinase 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PAK6, check out the PAK6 Infographic

PAK6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PAK6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-PAK6 Antibody

Question

We are currently using anti-PAK6 antibody PA1729 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

The anti-PAK6 antibody (PA1729) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-24

Question

Will anti-PAK6 antibody PA1729 work on canine WB with brain?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

Our lab technicians have not tested anti-PAK6 antibody PA1729 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PAK6 antibody PA1729 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-11

Question

Is there a BSA free version of anti-PAK6 antibody PA1729 available?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PAK6 antibody PA1729 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-03

Question

Would anti-PAK6 antibody PA1729 work for WB with prefrontal cortex?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

According to the expression profile of prefrontal cortex, PAK6 is highly expressed in prefrontal cortex. So, it is likely that anti-PAK6 antibody PA1729 will work for WB with prefrontal cortex.

Boster Scientific Support

Answered: 2019-12-19

Question

I have a question about product PA1729, anti-PAK6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-05

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1729 anti-PAK6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-05

Question

See below the WB image, lot number and protocol we used for prefrontal cortex using anti-PAK6 antibody PA1729. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-08-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-23

Question

Is this PA1729 anti-PAK6 antibody reactive to the isotypes of PAK6?

S. Patel

Verified customer

Asked: 2015-11-11

Answer

The immunogen of PA1729 anti-PAK6 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6(16-32aa QNFQHRVHTSFDPKEGK), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-11-11

Order DetailsPrice
PA1729

100μg

$370
PA1729-10ug

10μg sample (liquid)

$99
PA1729-Biotin

100 μg Biotin conjugated

$570
PA1729-Cy3

100 μg Cy3 conjugated

$570
PA1729-Dylight488

100 μg Dylight488 conjugated

$570
PA1729-Dylight550

100 μg Dylight550 conjugated

$570
PA1729-Dylight594

100 μg Dylight594 conjugated

$570
PA1729-FITC

100 μg FITC conjugated

$570
PA1729-HRP

100 μg HRP conjugated

$570
PA1729-APC

100 μg APC conjugated

$670
PA1729-PE

100 μg PE conjugated

$670
PA1729-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1729
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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