DARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA Kit

DARPP-32 ELISA kit, phospho-specific, cell-based

The DARPP-32 (Phospho-Thr75) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DARPP-32 (Phospho-Thr75) protein expression profile in cells. The kit can be used for measuring the relative amounts of DARPP-32 (Phospho-Thr75) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DARPP-32 (Phospho-Thr75).

Product Info Summary

SKU: EKC1938
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

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Product Name

DARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA Kit

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SKU/Catalog Number

EKC1938

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The DARPP-32 (Phospho-Thr75) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DARPP-32 (Phospho-Thr75) protein expression profile in cells. The kit can be used for measuring the relative amounts of DARPP-32 (Phospho-Thr75) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DARPP-32 (Phospho-Thr75).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

DARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1938)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1938 is reactive to Ppp1r1b in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1938 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Ppp1r1b (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-Ppp1r1b Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For Ppp1r1b (Source: Uniprot.Org, NCBI)

Gene Name

Ppp1r1b

Full Name

Protein phosphatase 1 regulatory subunit 1B

Weight

21.781kDa

Superfamily

protein phosphatase inhibitor 1 family

Alternative Names

DARPP32; DARPP-32; PPP1R1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32) Ppp1r1b|AU040756, DARP, DARPP-32, Dar, Darpp32|protein phosphatase 1, regulatory inhibitor subunit 1B|protein phosphatase 1 regulatory subunit 1B|dopamine- and cAMP-regulated neuronal phosphoprotein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ppp1r1b, check out the Ppp1r1b Infographic

Ppp1r1b infographic

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In this infographic you will see the following information for Ppp1r1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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