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Product Info Summary
| SKU: | A04319-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PPM1K/Pp2C Kappa Antibody
SKU/Catalog Number
A04319-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PPM1K/Pp2C Kappa Antibody (Catalog# A04319-1). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPM1K/Pp2C Kappa Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04319-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PPM1K. Immunogen sequence location: 205 - 254
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04319-1 is reactive to PPM1K in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
41.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04319-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using PP2Cκ Polyclonal Antibody
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Western blot analysis of the lysates from HeLa cells using PPM1K antibody.
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Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using PPM1K Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PPM1K/Pp2C Kappa Antibody (A04319-1)
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-PPM1K/Pp2C Kappa Antibody
Question
Is this A04319-1 anti-PPM1K/Pp2C Kappa antibody reactive to the isotypes of PPM1K?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
The immunogen of A04319-1 anti-PPM1K/Pp2C Kappa antibody is A peptide derived from human PPM1K. Immunogen sequence location: 205 - 254. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-24
Question
We are currently using anti-PPM1K/Pp2C Kappa antibody A04319-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on primate tissues as well?
B. Moore
Verified customer
Asked: 2017-08-22
Answer
The anti-PPM1K/Pp2C Kappa antibody (A04319-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-22
