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Product Info Summary
| SKU: | LS084259 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (DCUN1D5) |
Product info
Product Name
DCUN1D5 (NM_032299) Human Over-expression Lysate
SKU/Catalog Number
LS084259
Size
100 µg
Description
Transient overexpression lysate of DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (DCUN1D5)
Storage & Handling
Cite This Product
DCUN1D5 (NM_032299) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084259)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DCUN1D5 Human Over-expression Lysates NM_032299.
Western blot validation of overexpression lysate (LS084259) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DCUN1D5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Dcun1d5 (Source: Uniprot.org, NCBI)
Gene Name
Dcun1d5
Full Name
DCN1-like protein 5
Weight
27.579kDa
Alternative Names
DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae); DCN1-like protein 5; DCUN1 domain-containing protein 5; Defective in cullin neddylation protein 1-like protein 5; FLJ32431; FLJ37425; MGC2714
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Dcun1d5, check out the Dcun1d5 Infographic
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In this infographic, you will see the following information for Dcun1d5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DCUN1D5 (NM_032299) Human Over-expression Lysate (LS084259)
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No publications found for LS084259
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