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Product Info Summary
| SKU: | EK0964-CN |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Canine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Dog Canine IL-29 ELISA Kit PicoKine®
SKU/Catalog Number
EK0964-CN
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Dog Canine IL-29 ELISA Kit PicoKine® (96 Tests). Quantitate Dog IFNL1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Dog Canine IL-29 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0964-CN)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G20-T200
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0964-CN for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0964-CN is reactive to IFNL1 in Canine samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0964-CN is guaranteed for ELISA in Canine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IFNL1
Interferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in Dog Canineas is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0964-CN-CAP | Anti-Canine IFNL1 Pre-coated 96-well strip microplate | 1 |
| EK0964-CN-ST | Canine IFNL1 Standard | 2 vials, 10 ng/tube |
| EK0964-CN-DA | Canine IFNL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.034 | 0.07 | 0.119 | 0.208 | 0.4 | 0.736 | 1.252 | 2.03 |
Data Example Images
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Dog IL-29 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Dog Canine IL-29 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 90 | 415 | 1566 | 103 | 448 | 1895 |
| Standard deviation | 3.78 | 22.00 | 72.04 | 4.94 | 28.67 | 138.34 |
| CV (%) | 4.2% | 5.3% | 4.6% | 4.8% | 6.4% | 7.3% |
Specific Publications For Dog Canine IL-29 ELISA Kit PicoKine® (EK0964-CN)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Dog Canine IL-29 ELISA Kit PicoKine®
Question
Q: how to analyze ELISA data? I have performed IL-29 level in plasma.
R. Evans
Verified customer
Asked: 2020-01-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-01-09
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Martinez
Verified customer
Asked: 2019-07-29
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-07-29
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-03-11
Question
Q: can I use citrate plasma as samples in Dog IL-29 Picokine® ELISA Kit (Catalog # EK0964-CN)?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-29 causing degradation of its protein structure. IL-29 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-29 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-10-31
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-29 using the EK0964-CN Dog IL-29 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-02-16
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2017-02-16
Question
Q: if the enzyme conjugated IL-29 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-29 antigen?
Verified Customer
Verified customer
Asked: 2015-06-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2015-06-14
