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Product Info Summary
| SKU: | LS001847 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of dual specificity phosphatase 5 (DUSP5) |
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Product info
Product Name
DUSP5 (NM_004419) Human Over-expression Lysate
SKU/Catalog Number
LS001847
Size
100 µg
Description
Transient overexpression lysate of dual specificity phosphatase 5 (DUSP5)
Storage & Handling
Cite This Product
DUSP5 (NM_004419) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001847)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DUSP5 Human Over-expression Lysates NM_004419.
Western blot validation of overexpression lysate (LS001847) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DUSP5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for DUSP5 (Source: Uniprot.org, NCBI)
Gene Name
DUSP5
Full Name
Dual specificity protein phosphatase 5
Weight
42.047kDa
Superfamily
protein-tyrosine phosphatase family
Alternative Names
dual specificity phosphatase 5; dual specificity protein phosphatase 5; Dual specificity protein phosphatase hVH3; DUSP; EC 3.1.3.16; EC 3.1.3.48; HVH3VH1-like phosphatase 3; serine/threonine specific protein phosphatase; VH3 DUSP5 DUSP, HVH3 dual specificity phosphatase 5 dual specificity protein phosphatase 5|VH1-like phosphatase 3|dual specificity protein phosphatase hVH3|serine/threonine specific protein phosphatase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DUSP5, check out the DUSP5 Infographic
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In this infographic, you will see the following information for DUSP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DUSP5 (NM_004419) Human Over-expression Lysate (LS001847)
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