EAAT4 (SLC1A6) (NM_005071) Human Over-expression Lysate

Lysate of EAAT4 over-expression cell lines, WB control

Transient overexpression lysate of solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6)

Product Info Summary

SKU: LS006511
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6)

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Product Name

EAAT4 (SLC1A6) (NM_005071) Human Over-expression Lysate

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SKU/Catalog Number

LS006511

Size

100 µg

Description

Transient overexpression lysate of solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6)

Storage & Handling

Cite This Product

EAAT4 (SLC1A6) (NM_005071) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006511)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SLC1A6 (Source: Uniprot.org, NCBI)

Gene Name

SLC1A6

Full Name

Excitatory amino acid transporter 4

Weight

61.565kDa

Superfamily

dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family

Alternative Names

EAAT4MGC43671; excitatory amino acid transporter 4; MGC33092; Sodium-dependent glutamate/aspartate transporter; solute carrier family 1 (high affinity aspartate/glutamate transporter), member6; Solute carrier family 1 member 6 SLC1A6 EAAT4 solute carrier family 1 member 6 excitatory amino acid transporter 4|sodium-dependent glutamate/aspartate transporter|solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SLC1A6, check out the SLC1A6 Infographic

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In this infographic, you will see the following information for SLC1A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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