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Product Info Summary
| SKU: | LS008664 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of eukaryotic translation initiation factor 3, subunit D (EIF3D) |
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Product info
Product Name
EIF3D (NM_003753) Human Over-expression Lysate
SKU/Catalog Number
LS008664
Size
100 µg
Description
Transient overexpression lysate of eukaryotic translation initiation factor 3, subunit D (EIF3D)
Storage & Handling
Cite This Product
EIF3D (NM_003753) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008664)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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EIF3D Human Over-expression Lysates NM_003753.
Western blot validation of overexpression lysate (LS008664) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with EIF3D (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for EIF3D (Source: Uniprot.org, NCBI)
Gene Name
EIF3D
Full Name
Eukaryotic translation initiation factor 3 subunit D
Weight
63.973kDa
Superfamily
eIF-3 subunit D family
Alternative Names
eIF3d; eIF3-p66; EIF3S7eIF3 p66; eIF-3-zeta; eIF3-zeta; Eukaryotic translation initiation factor 3 subunit 7; eukaryotic translation initiation factor 3 subunit D; eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; eukaryotic translation initiation factor 3, subunit D; MGC126526; MGC17258; translation initiation factor eIF3 p66 subunit EIF3D EIF3S7, eIF3-p66, eIF3-zeta eukaryotic translation initiation factor 3 subunit D eukaryotic translation initiation factor 3 subunit D|eIF3 p66|eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa|translation initiation factor eIF3 p66 subunit
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on EIF3D, check out the EIF3D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF3D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For EIF3D (NM_003753) Human Over-expression Lysate (LS008664)
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No publications found for LS008664
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