Anti-eIF3 Zeta (R135) EIF3D Antibody

Product Name

Anti-eIF3 Zeta (R135) EIF3D Antibody

View all EIF3D Antibodies

SKU/Catalog Number

A06212-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-eIF3 Zeta (R135) EIF3D Antibody catalog # A06212-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eIF3 Zeta (R135) EIF3D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06212-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human EIF3D.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06212-1 is reactive to EIF3D in Human, Mouse, Rat

Applications

A06212-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

63973 MW

Background of EIF3D

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. HAMAP-Rule MF_03003

Asano K., J. Biol. Chem. 272:27042-27052(1997).
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of EIF3D using Anti-eIF3 Zeta (R135) EIF3D Antibody (A06212-1).

Immunohistochemistry (IHC) analyzes of eIF3ζ (R135) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-eIF3 Zeta (R135) EIF3D Antibody A06212-1

Western blot (WB) analysis of eIF3ζ (R135) pAb at 1:1000 dilution
Lane1:The Brain tissue lysate of Mouse(40ug)
Lane2:The Brain tissue lysate of Rat(20ug)
Lane3:HEK293T whole cell lysate(10ug)
Lane4:MCF-7 whole cell lysate(20ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For EIF3D (Source: Uniprot.org, NCBI)

Alternative Names

eIF3d; eIF3-p66; EIF3S7eIF3 p66; eIF-3-zeta; eIF3-zeta; Eukaryotic translation initiation factor 3 subunit 7; eukaryotic translation initiation factor 3 subunit D; eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; eukaryotic translation initiation factor 3, subunit D; MGC126526; MGC17258; translation initiation factor eIF3 p66 subunit EIF3D EIF3S7, eIF3-p66, eIF3-zeta eukaryotic translation initiation factor 3 subunit D eukaryotic translation initiation factor 3 subunit D|eIF3 p66|eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa|translation initiation factor eIF3 p66 subunit

For more info on EIF3D, check out the EIF3D Infographic

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In this infographic, you will see the following information for EIF3D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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