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PicoKine® ELISA Kits
Browse PicoKine® ELISA Kits
High-sensitivity ELISA kits for protein quantification
Popular PicoKine® Targets
Looking for other ELISA formats?
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Go to the ELISA Kits HubBrowse picokine elisa kits
Product
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Species
Applications
Price
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Human
ELISA
$499
Cat # EK2388
96 wells/kit, with removable strips.
Cat # EK2388
96 wells/kit, with removable strips.
1 images
Human
ELISA
$499
Cat # EK2385-1
96 wells/kit, with removable strips.
Cat # EK2385-1
96 wells/kit, with removable strips.
1 images
Mouse
ELISA
$499
Cat # EK2386
96 wells/kit, with removable strips.
Cat # EK2386
96 wells/kit, with removable strips.
1 images
Mouse
ELISA
$499
Cat # EK0585
96 wells/kit, with removable strips.
Cat # EK0585
96 wells/kit, with removable strips.
1 images
Human
ELISA
$750
Cat # EK2222
96 wells/kit, with removable strips.
Cat # EK2222
96 wells/kit, with removable strips.
1 images
Human
ELISA
$750
Cat # EK2219
96 wells/kit, with removable strips.
Cat # EK2219
96 wells/kit, with removable strips.
1 images
Human
ELISA
$750
Cat # EK2220
96 wells/kit, with removable strips.
Cat # EK2220
96 wells/kit, with removable strips.
1 images
Human
ELISA
$499
Cat # EK2221
96 wells/kit, with removable strips.
Cat # EK2221
96 wells/kit, with removable strips.
1 images
Human
ELISA
$499
Cat # EK2223
96 wells/kit, with removable strips.
Cat # EK2223
96 wells/kit, with removable strips.
1 images
Human
ELISA
$750
Cat # EK2218
96 wells/kit, with removable strips.
Cat # EK2218
96 wells/kit, with removable strips.
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PicoKine® ELISA Kits Trusted in Publications Worldwide
Boster Biological Technology has more than 30 years of experience in manufacturing ELISA kits and antibodies. PicoKine® ELISA kits are developed for researchers who need dependable protein quantification with a standardized, ready-to-run workflow and consistent in-house quality control.
Across Boster’s ELISA portfolio, kits are validated for key performance characteristics such as specificity, sensitivity, stability, and lot-to-lot consistency. This helps support reliable data generation in routine research and publication-focused work.
When PicoKine® Is a Good Fit
PicoKine® is a good choice when you want a ready-to-run sandwich ELISA rather than a more customizable or development-heavy format.
- Suitable for common protein biomarker workflows
- Frequently used with serum, plasma, cell culture supernatant, and tissue homogenate
- Useful when reproducibility and ease of setup matter more than assay customization
Best suited for researchers who prioritize reproducibility, convenience, and a more standardized workflow.
Why Researchers Choose PicoKine® ELISA Kits
Key features of Boster’s PicoKine® ELISA kits
High Sensitivity for Low-Abundance Targets
Boster's Picokine® ELISA kits are made with high affinity antibodies that can detect native form proteins with picogram and subpicogram level sensitivity.
QC-Verified Specificity
Boster's QC department validates our ELISA kits against proteins in relevant superfamilies and proteins with similar immunogenicities to ensure specificity to the analytes of interests.
Broad PicoKine® Target Coverage
Our PicoKine® ELISA kits cover a wide range of validated targets and sample matrices, from serum and plasma to cell culture supernatant and tissue homogenate, helping you find the right assay for your workflow.
Technical Support You Can Rely On
Boster has been serving the research community since 1993 and cited by 70,000+ publications. Our team of experts are dedicated to provide you the best customer service.
Typical PicoKine® ELISA Workflow
PicoKine® ELISA kits follow a standard sandwich ELISA workflow designed for sensitive and reproducible protein quantification.
Plate Coating / Capture Setup
The assay plate is prepared with the capture component needed to immobilize the target analyte for detection.Blocking
Unbound sites are blocked to reduce non-specific binding and improve assay background.Target Binding and Detection
The analyte is captured and detected through the ELISA binding system, followed by enzyme-linked signal generation.Signal Development and Readout
Substrate is added and the resulting signal is measured to quantify the target concentration.Why Researchers Trust Boster
Track record, publication support, and scale behind Boster’s ELISA and antibody portfolio
Trusted by Researchers Worldwide
Publication-backed performance across Boster’s ELISA portfolio
Boster’s PicoKine® ELISA kits alone are associated with an estimated 6000+ publications, reflecting broad use in peer-reviewed research. You can explore specific citations on individual product pages.
Testimonials
One example of researcher feedback on Boster ELISA performance.
Sanjay Arora
Graduate Student
Quantification Of BDNF Post-Transfection In Primary Neuronal Cells
The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again.
ELISA , Cell lysate and culture medium
Need ELISA learning resources?
Explore ELISA principles, protocols, troubleshooting, optimization tips, and data analysis resources related to PicoKine® workflows.
This section highlights practical learning content for assay setup, optimization, and result interpretation.
PicoKine® ELISA FAQs
For PicoKine®-specific questions not covered here, contact support@bosterbio.com.
Q.1 How do I troubleshoot low signal in PicoKine® ELISA results?
A number of problems commonly arise during ELISA that can result in low signal and poor color development. Use our troubleshooting tips to help you improve your experiment and get better data. For more information, visit
ELISA Troubleshooting Low Signal.
Q.2 What is matrix effect and how does it affect PicoKine® ELISA results?
Matrix effect occurs when the target antigen interacts with matrix components in plasma or serum samples. These matrix components can be endogenous biological components such as phospholipids, carbohydrates, and metabolites. Matrix components can reduce the binding of the antibody to the target protein, or non-specifically bind the antibody, generating weak or noisy results.
Read some tips to reduce matrix effect.
Q.3 How many samples can I run on a 96-well PicoKine® ELISA plate?
On each 96 well plate, we recommend running an 8-point standard curve with duplicate wells. With the remaining 80 wells, 40 samples can be tested in duplicate.
Learn more about the number of samples tested per microtiter plate.
Q.4 Is the plate separable? Can I use only part of the PicoKine® kit and save the rest for later?
Yes, the ELISA kit plate is separable if the kit description says that there are removable strips. There are 12 strips of 8 wells each, all removable from the plate.
Q.5 The kit does not include wash solution. What should I do?
Our ELISA kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder, SKU: AR0030).
Q.6 Will this PicoKine® ELISA kit work on tissue samples?
For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is endogenous biotin in the tissue lysate samples using the ELISA kit. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, then you can work on the tissue sample by using the kit.
Our kits should still be stable after being in ambient temperatures for around 5 days. It would not affect the performance of the kit. We suggest you run the test and contact us if it does not work as expected.
Bubbles in the wells are uncommon, but they typically do not affect the results of your experiment. Prior to the assay, we suggest washing the plate to remove the bubbles as follows: wash the plate 3 times with 1X wash buffer, discard the liquid, invert the plate onto a paper towel, and gently tap to blot remaining liquid. It is recommended that the wells should not be completely dry at any time. Add 300 µl of 1X wash buffer to each assay well. For cleaner background, incubate for 60 seconds between each wash. Repeat these steps 2 additional times.
Continue Learning
Explore ELISA technical content related to PicoKine® workflows, assay setup, troubleshooting, and data analysis.
ELISA Fundamental Principle, How ELISA Works
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface...
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ELISA Sample Preparation Guide
The procedure below provides a general guidance for the preparation of commonly tested samples for use in ELISA assays. Please check with the literature for experiments similar to yours for your new assay development. In this article...
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An ELISA Guide That You Cannot Miss
This guide will teach you everything you need to become an ELISA expert, including critical review of principle, all-in-one FAQs, and more...
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Boster's Free Troubleshooting Guides
You are at the right place and at the right time for our latest series of troubleshooting handbooks. They are available at your finger tips with just a few clicks...
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Guidelines for Preparing ELISA Standards
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay used to detect the concentration of a specific protein in a liquid sample. Three different types of data output can be obtained...
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Four Types of ELISA Assay
Are you familiar with the multiple methods you could use to perform an ELISA? Among the standard assay formats illustrated below, where differences in...
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ELISA Troubleshooting: Weak Signal
Weak or no color development in an ELISA assay can indicate that the target protein is present in minute quantities in the sample, if at all. It can also mean that there is something wrong with the assay or the reagents that prevents efficient detection....
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ELISA Blocking Optimization
The process of coating an ELISA plate relies on the passive binding activity of the solid phase, which immobilizes biomolecules on the well surface. Without appropriate blocking, the plate would bind the detection antibody alongside the antigen or detection...
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ELISA Data Analysis
After performing an ELISA with a ready-to-use ELISA kit or an antibody pair kit, the data generated must be analyzed to quantitate the target protein concentrations. We discuss the different aspects to consider for more consistent and accurate ELISA data. Furthermore, we provide a step-by-step guide to create the standard curve for analysis.....
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Pitfalls to Avoid for ELISA!
ELISA (enzyme-linked immunosorbent assay) is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies, and hormones in samples, rendering it as one of the most widely used immunoassays. Despite the many advantages, there are some mistakes that could turn your ELISA experiment sour...
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ELISA Blocking Buffer Optimization
There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between....
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How to Perfect Your ELISA Standards: Protocol and Troubleshooting
At Boster, one common question we get from researchers is, “How do I prepare the ELISA standard?” We’re glad you asked because proper construction of the standard curve is the very first step....
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