Human IL-10/Interleukin-10 ELISA Kit PicoKine™

Human IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL10 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.5pg/ml. Cited in 71 publication(s).

Product Info Summary

SKU: EK0416
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Product Name

Human IL-10/Interleukin-10 ELISA Kit PicoKine™

See all IL-10 products

SKU/Catalog Number

EK0416

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL10 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-10/Interleukin-10 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0416)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-N178

Sensitivity

<0.5 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0416 is reactive to IL10 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Application Guarantee

EK0416 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0416-CAP Anti-Human IL10 Pre-coated 96-well strip microplate 1
EK0416-ST Human IL10 Standard 2 vials, 10 ng/tube
EK0416-DA Human IL10 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.0600.1010.1510.1870.2950.5380.8581.722

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-10, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-10 in samples: 21878171 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-10/Interleukin-10 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)16531131554122
Standard deviation1.022.127.341.243.029.88
CV (%)6.4%4%6.5%8.3%5.6%8.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-10 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 116171515150.825.4%
Sample 253454748482.946.1%
Sample 31131231061041117.436.6%
*number of samples for each test n=16.

Gene/Protein Information For IL10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P22301

Gene ID

3586

Gene Name

IL10

Full Name

Interleukin-10

Weight

20.517kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL10, check out the IL10 Infographic

IL10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0416 has been cited in 71 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Singh A,Das D,Kurra S,Arava S,Gupta S,Sharma A.Dendritic cells and their associated pro-inflammatory cytokines augment to the inflammatory milieu in vitiligo skin.Cytokine.2021 Jun 5:155598.doi:10.1016/j.cyto.2021.155598.Epub ahead of print.PMID:34103210.
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:DENDRITIC CELL, DILUTION:NA

Yu D,Zhao Y,Wang H,Kong D,Jin W,Hu Y,Qin Y,Zhang B,Li X,Hao J,Li G,Wang H.IL-1β pre-stimulation enhances the therapeutic effects of endometrial regenerative cells on experimental colitis. Stem Cell Res Ther.2021 Jun 5;12(1):324.doi:10.1186/s13287-021-02392-9.PMID:34090510.
Species: Human,Mouse
EK0416 usage in article: APP:ELISA, SAMPLE:ERCS, DILUTION:NA

Zedan MM,Zedan MM,El-Said AM,Badran AM,Wahba Y.Evaluation of serum levels of interleukin-10 and transforming growth factor-β1 among Egyptian children with wheezy asthma phenotype.Egypt J Chest Dis Tuberc 2021;70:208-14
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Chen J,Wang H,Jia L,He J,Li Y,Liu H,Wu R,Qiu Y,Zhan Y,Yuan Z,Cao Y,Li W,Xu K,Yin P.Bufalin targets the SRC-3/MIF pathway in chemoresistant cells to regulate M2 macrophage polarization in colorectal cancer.Cancer Lett.2021 May 14:S0304-3835(21)00223-8.doi:10.1016/j.canlet.2021.05.008.Epub ahead of print.PMID:34000344.
Species: Human,Mouse
EK0416 usage in article: APP:ELISA, SAMPLE:CRC CELL, DILUTION:NA

Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
Species: Human,Mouse
EK0416 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA

Guo L,Jiang B,Li D,Xiao X. Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor.Drug Des Devel Ther.2021 Feb 16;15:589-600.doi:10. 2147/DDDT.S294009.PMID:33623368;PMCID
Species: Rat
EK0416 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

The effectiveness of olmesartan on inflammation at cardiopulmonary bypass. Ibrahim Murat Ozguler,Latif Ustunel
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Casciaro F,Zia S,Forcato M,Zavatti M,Beretti F,Bertucci E,Zattoni A,Reschiglian P,Alviano F,Bonsi L,Follo MY,Demaria M,Roda B,Maraldi T.Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations.Cells.2021 Jan 15;10(1):158.doi:
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:HAFSCS, DILUTION:NA

Liu H,Chen W,Zhao B,Quan W,Zhang Y,Zhou Y,Wan Z,Zhang X,Xue G,Li J,Luo S,Wang J,Liu Y,Zhen M,Zhao Y.Autologous bionic tissue for inguinal hernia repair.J Biomed Mater Res A.2020 Jun; 108(6):1351-1368.doi:10.1002/jbm.a.36907.Epub 2020 Mar 2.PMID:32090432.
Species: New Zealand white Rabbit
EK0416 usage in article: APP:ELISA, SAMPLE:MSCS, DILUTION:NA

Das D, Singh A, Antil PS, Sharma D, Arava S, Khandpur S, Sharma A. Distorted frequency of dendritic cells and their associated stimulatory and inhibitory markers augment the pathogenesis of pemphigus vulgaris. Immunol Res. 2020 Nov 12. doi: 10.1007/s12026
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:PBMCS, DILUTION:NA

Duan, Y., Zheng, H., Li, Z., Yao, Y., Ding, J., Wang, X., ... & Zheng, X. (2020). Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis. Biomaterials, 120012.

The Impact of Pre-Cooling and CoQ 10 Supplementation on Mediators of Inflammatory Cytokines in Elite Swimmers

Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis

Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis

Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in vitro stimulation with ?-cell autoantigens

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma

Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome

Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica

Evaluation of Interleukin-10 Levels in Patients Diagnosed with Chronic Hepatitis

Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells

Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155

Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages

Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery

Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients

Immunomodulatory effects of total intravenous and balanced inhalation anesthesia in patients with bladder cancer undergoing elective radical Cystectomy: preliminary results

HER2-specific T lymphocytes kill both trastuzumab-resistant and trastuzumab-sensitive breast cell lines in vitro

Dang, W., Xie, W. & Zhou, J. (2017). Expression of PYCARD Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Primary Gout Patients with Different Chinese Medicine Syndromes. Chinese Journal of Integrative Medicine, 1-8. Advance ...

He, S., Le, N.A., Frediani, J.K., Winterberg, P.D., Jin, R., Liverman, R.,..., & Vos, M.B. (2017). Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. ...

Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.

Luo H,, Wang X,, Wang J,, Chen C,, Wang N,, Xu Z,, Chen S,, Zeng C,. Cardiovasc Diabetol. 2015 Jun 10;14:76. Doi: 10.1186/S12933-015-0239-7. Chronic Nf-??b Blockade Improves Renal Angiotensin Ii Type 1 Receptor Functions And Reduces Blood Pressure...

Wang T, Zhou Yt, Chen Xn, Zhu Ax, Wu Bh. World J Gastroenterol. 2014 Jul 28;20(28):9519-27. Doi: 10.3748/Wjg.V20.I28.9519. Remote Ischemic Postconditioning Protects Against Gastric Mucosal Lesions In Rats.

Zhang X, Liu F, Liu H, Cheng H, Wang W, Wen Q, Wang Y. Inflamm Res. 2011 Jun;60(6):569-75. Doi: 10.1007/S00011-010-0305-2. Epub 2011 Jan 19. Urinary Trypsin Inhibitor Attenuates Lipopolysaccharide-Induced Acute Lung Injury By Blocking The Activati...

Chen T, Liu W, Chao X, Zhang L, Qu Y, Huo J, Fei Z. Brain Res Bull. 2011 Feb 1;84(2):163-8. Doi: 10.1016/J.Brainresbull.2010.11.015. Epub 2010 Dec 4. Salvianolic Acid B Attenuates Brain Damage And Inflammation After Traumatic Brain Injury In Mice.

Li Xj, Liu Dp, Chen Hl, Pan Xh, Kong Qy, Pang Qf. Int Immunopharmacol. 2012 Feb;12(2):460-4. Doi: 10.1016/J.Intimp.2012.01.001. Epub 2012 Jan 15. Lactoferrin Protects Against Lipopolysaccharide-Induced Acute Lung Injury In Mice.

Xia J, Zhou Xl, Zhao Y, Zhu Yq, Jiang S, Ni Sz. Inflammation. 2013 Dec;36(6):1431-9. Doi: 10.1007/S10753-013-9683-2. Roles Of Lipoxin A4 In Preventing Paracetamol-Induced Acute Hepatic Injury In A Rabbit Model.

Tang Y, Liao Y, Kawaguchi-Sakita N, Raut V, Fakhrejahani E, Qian N, Toi M. J Hepatobiliary Pancreat Sci. 2011 Jul;18(4):551-8. Doi: 10.1007/S00534-010-0368-Z. Sinisan, A Traditional Chinese Medicine, Attenuates Experimental Chronic Pancreatitis In...

Chen W, Qi J, Feng F, Wang Md, Bao G, Wang T, Xiang M, Xie Wf. Neurochem Int. 2014 Mar;68:28-37. Doi: 10.1016/J.Neuint.2014.01.015. Epub 2014 Feb 12. Neuroprotective Effect Of Allicin Against Traumatic Brain Injury Via Akt/Endothelial Nitric Oxide...

Ye Xh, Wu Y, Guo Pp, Wang J, Yuan Sy, Shang Y, Yao Sl. Brain Res. 2010 Apr 6;1323:174-83. Doi: 10.1016/J.Brainres.2010.01.079. Epub 2010 Feb 4. Lipoxin A4 Analogue Protects Brain And Reduces Inflammation In A Rat Model Of Focal Cerebral Ischemia R...

Jin Y, Wan Y, Chen G, Chen L, Zhang Mq, Deng L, Zhang Jc, Xiong Xz, Xin Jb. Plos One. 2014 Oct 16;9(10):E111044. Doi: 10.1371/Journal.Pone.0111044. Ecollection 2014. Treg/Il-17 Ratio And Treg Differentiation In Patients With Copd.

Sereshki N, Gharagozloo M, Ostadi V, Ghahiri A, Roghaei Ma, Mehrabian F, Andalib Aa, Hassanzadeh A, Hosseini H, Rezaei A. Int J Fertil Steril. 2014 Apr;8(1):59-66. Epub 2014 Mar 9. Variations In T-Helper 17 And Regulatory T Cells During The Menstr...

Hualin C, Wenli X, Dapeng L, Xijing L, Xiuhua P, Qingfeng P. Inflammation. 2012 Jun;35(3):1087-93. Doi: 10.1007/S10753-011-9415-4. The Anti-Inflammatory Mechanism Of Heme Oxygenase-1 Induced By Hemin In Primary Rat Alveolar Macrophages.

Zhou X, Cao Y, Ao G, Hu L, Liu H, Wu J, Wang X, Jin M, Zheng S, Zhen X, Alkayed Nj, Jia J, Cheng J. Antioxid Redox Signal. 2014 Oct 20;21(12):1741-58. Doi: 10.1089/Ars.2013.5587. Epub 2014 Mar 13. Camkk??-Dependent Activation Of Amp-Activated Prot...

Sun Hx, Chen Lq, Zhang J, Chen Fy. J Ethnopharmacol. 2014 May 14;153(3):831-9. Doi: 10.1016/J.Jep.2014.03.052. Epub 2014 Mar 29. Anti-Tumor And Immunomodulatory Activity Of Peptide Fraction From The Larvae Of Musca Domestica.

Ruan Z, Liu S, Zhou Y, Mi S, Liu G, Wu X, Yao K, Assaad H, Deng Z, Hou Y, Wu G, Yin Y. Plos One. 2014 Jun 2;9(6):E97815. Doi: 10.1371/Journal.Pone.0097815. Ecollection 2014. Chlorogenic Acid Decreases Intestinal Permeability And Increases Expressi...

Meng A, Wang B, Zhang X, Qi N, Liu D, Wu J. Inflammation. 2015;38(3):1260-6. Doi: 10.1007/S10753-014-0093-X. Additive Suppression Of Lps-Induced Il-10 And Tnf-?? By Pre-Treatment Of Dexamethasone And Sb203580 In A Murine Alveolar Macrophage Cell L...

Chen W, Guo Y, Yang W, Zheng P, Zeng J, Tong W. Exp Brain Res. 2015 Oct;233(10):2823-31. Doi: 10.1007/S00221-015-4352-3. Epub 2015 Jun 13. Protective Effect Of Ginsenoside Rb1 On Integrity Of Blood-Brain Barrier Following Cerebral Ischemia.

Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...

Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...

Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.

Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...

Yin Y, Qin T, Yu Q, Yang Q. Amino Acids. 2014 Jul;46(7):1763-74. Doi: 10.1007/S00726-014-1735-X. Epub 2014 Apr 8. Bursopentin (Bp5) From Chicken Bursa Of Fabricius Attenuates The Immune Function Of Dendritic Cells.

Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...

Chen X, Zhao C, Li X, Wang T, Li Y, Cao C, Ding Y, Dong M, Finci L, Wang Jh, Li X, Liu L. Nat Chem Biol. 2015 Jan;11(1):19-25. Doi: 10.1038/Nchembio.1657. Epub 2014 Nov 10. Terazosin Activates Pgk1 And Hsp90 To Promote Stress Resistance.

Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...

Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.

Zhang Y, Zhang S, Li H, Huang M, Xu W, Chu K, Chen L, Chen X. Mol Med Rep. 2015 Jul;12(1):988-94. Doi: 10.3892/Mmr.2015.3515. Epub 2015 Mar 19. Ameliorative Effects Of Gualou Guizhi Decoction On Inflammation In Focal Cerebral Ischemic-Reperfusion ...

Shi Jq, Chen J, Wang Br, Zhu Yw, Xu Y, Wang J, Xiao H, Shi Jp, Zhang Yd, Xu J. Peptides. 2011 Aug;32(8):1617-25. Doi: 10.1016/J.Peptides.2011.06.013. Epub 2011 Jun 21. Short Amyloid-Beta Immunogens Show Strong Immunogenicity And Avoid Stimulating ...

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Wang Sb, Deng Yq, Ren J, Xiao Bk, Chen Z, Tao Zz. Scand J Immunol. 2013 Dec;78(6):507-15. Doi: 10.1111/Sji.12118. Lactoferrin Administration Into The Nostril Alleviates Murine Allergic Rhinitis And Its Mechanisms.

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Jin Q, Cheng J, Liu Y, Wu J, Wang X, Wei S, Zhou X, Qin Z, Jia J, Zhen X. Brain Behav Immun. 2014 Aug;40:131-42. Doi: 10.1016/J.Bbi.2014.03.003. Epub 2014 Mar 12. Improvement Of Functional Recovery By Chronic Metformin Treatment Is Associated With...

Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...

Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...

Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro.

Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????

Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...

Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...

Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...

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12 Customer Q&As for Human IL-10/Interleukin-10 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated IL-10 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-10 antigen?

Verified Customer

Verified customer

Asked: 2020-12-08

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-12-08

Question

Q: can you recommend the dilution ratio of serum samples for detection of IL-10 in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Y. Li

Verified customer

Asked: 2020-03-09

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-03-09

Question

Q: Can IL-10 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

K. Puri

Verified customer

Asked: 2019-07-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-07-23

Question

Have you tested EK0416 in tissue homogenates and Cell culture supernates?

Verified customer

Asked: 2019-03-15

Answer

The Human IL-10 EK0416 Elisa Kit has not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2019-03-18

Question

Q: is it okay to use EDTA plasma as samples in Human IL-10 Picokine® ELISA Kit (Catalog # EK0416)?

H. Smith

Verified customer

Asked: 2018-10-01

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-10 causing degradation of its protein structure. IL-10 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-10 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-10-01

Question

My sample type is cell supernates. I would like to know what ratio should I use to dilute my samples during using your product EK0416 and EK0525. I couldn't find any reference from the product page.

Verified Customer

Verified customer

Asked: 2018-08-08

Answer

For untreated cell supernatants, the suggested dilution ratio is 1:1. For treated cell supernatants, concentrations of the samples can vary greatly. We suggest the customer run pilot tests for different group samples to figure out the optimal dilution ratio.

Boster Scientific Support

Answered: 2018-08-08

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-08-05

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-08-05

Question

I am looking for IL 10 that works both with human and mouse is the EK0416 antibody kit will cross react with human and mouse?

Verified Customer

Verified customer

Asked: 2018-06-21

Answer

The EK0416 antibody kit did not show any cross-reactivity with mouse samples, therefore, this kit is not expected to cross react.

Boster Scientific Support

Answered: 2018-06-21

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-06-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-16

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-05-08

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

M. Walker

Verified customer

Asked: 2017-11-25

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-11-25

Question

Q: how can I thaw whole blood sample for IL-10 ELISA after freezing?

N. Moore

Verified customer

Asked: 2017-10-22

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-10 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-10-22

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