Click image to see more details
Product Info Summary
| SKU: | EK0416 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
Customers Who Bought This Also Bought
Product info
Product Name
Human IL-10/Interleukin-10 ELISA Kit PicoKine®
SKU/Catalog Number
EK0416
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL10 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-10/Interleukin-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0416)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S19-N178
Sensitivity
<0.5 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0416 is reactive to IL10 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Application Guarantee
EK0416 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-10
Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0416-CAP | Anti-Human IL10 Pre-coated 96-well strip microplate | 1 |
| EK0416-ST | Human IL10 Standard | 2 vials, 10 ng/tube |
| EK0416-DA | Human IL10 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.060 | 0.101 | 0.151 | 0.187 | 0.295 | 0.538 | 0.858 | 1.722 |
Data Example Images
Click image to see more details
Human IL-10 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-10, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of IL-10 in samples: 21878171 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-10/Interleukin-10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 16 | 53 | 113 | 15 | 54 | 122 |
| Standard deviation | 1.02 | 2.12 | 7.34 | 1.24 | 3.02 | 9.88 |
| CV (%) | 6.4% | 4% | 6.5% | 8.3% | 5.6% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-10 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 17 | 15 | 15 | 15 | 0.82 | 5.4% |
| Sample 2 | 53 | 45 | 47 | 48 | 48 | 2.94 | 6.1% |
| Sample 3 | 113 | 123 | 106 | 104 | 111 | 7.43 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For IL10 (Source: Uniprot.Org, NCBI)
Uniprot ID
P22301
Gene ID
3586
Gene Name
IL10
Full Name
Interleukin-10
Weight
20.517kDa
Superfamily
IL-10 family
Alternative Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL10, check out the IL10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human IL-10/Interleukin-10 ELISA Kit PicoKine® (EK0416)
EK0416 has been cited in 160 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155
Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery
Therapeutic effects of ciprofloxacin/bushenhuazhuo combination on chronic prostatitis
Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome
Bladder cancer cell‑secreted exosomal miR‑21 activates the PI3K/AKT pathway in macrophages to promote cancer progression
The effect of intravascular interventional embolization and craniotomy on MMP-2, MMP-9 and caspase3 in serum of intracranial aneurysm patients
Morphological and functional changes in bone marrow mesenchymal stem cells in rats with heart failure
Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome
Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations
FoxP3 - Tr1 Cell in Generalized Myasthenia Gravis and Its Relationship With the Anti-AChR Antibody and Immunomodulatory Cytokines
Tolerogenic Effect Elicited by Protein Fraction Derived From Different Formulas for Dietary Treatment of Cow’s Milk Allergy in Human Cells
What is the impact of SOCS3, IL-35 and IL17 in immune pathogenesis of recurrent pregnancy loss?
Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease
Serum levels of TGFβ, IL-10, IL-17, and IL-23 cytokines in β-thalassemia major patients: the impact of silymarin therapy
Analysis of IL-10 and IL-6 Gene Polymorphisms and Their Serum Levels in Patients with Brucellosis: A Case Control Study
MiR-140a contributes to the pro-atherosclerotic phenotype of macrophages by downregulating interleukin-10.
LncRNA MEG3 inhibits proliferation and promotes apoptosis of osteosarcoma cells through regulating Notch signaling pathway.
High glucose promotes prostate cancer cells apoptosis via Nrf2/ARE signaling pathway.
Platelet-rich plasma plays an antibacterial, anti-inflammatory and cell proliferation-promoting role in an in vitro model for diabetic infected wounds
Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor
Activation of Cholinergic Anti-Inflammatory Pathway in Peripheral Immune Cells Involved in Therapeutic Actions of α-Mangostin on Collagen-Induced Arthritis in Rats
In vitro modeling of COPD inflammation and limitation of p38 inhibitor – SB203580
Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma
LPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Behçet’s Disease
Immunomodulatory effects of total intravenous and balanced inhalation anesthesia in patients with bladder cancer undergoing elective radical Cystectomy: preliminary results
Expression of S100 calcium-binding protein A8 in peripheral blood of patients with preeclampsia during pregnancy:
Altered serum interleukin-7 and interleukin-10 are associated with drug-free major depressive disorder:
Identification and Validation of Gene Expression Pattern and Signature in Patients with Immune Thrombocytopenia:
Short-Term Effect of High-Dose Vitamin D on the Level of Interleukin 10 in Patients with Multiple Sclerosis: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
The Imbalance between Foxp3+Tregs and Th1/Th17/Th22 Cells in Patients with Newly Diagnosed Autoimmune Hepatitis
Integration of Biomechanical and Biological Characterization in the Development of Porous Poly(caprolactone)-Based Membranes for Abdominal Wall Hernia Treatment
Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by Mycobacterium avium-Infected Macrophages
Inhibition of EZH2 attenuates coronary heart disease by interacting with microRNA‐22 to regulate the TXNIP/nuclear factor‐κB pathway
Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross‐sectional, single‐center study in the USA
IRF4 silencing inhibits Hodgkin lymphoma cell proliferation, survival and CCL5 secretion
IL‐10 polymorphisms and T‐cell subsets could affect the clinical presentation and outcome of childhood immune thrombocytopenia in Egyptian population
MicroRNA profiling in Chinese children with Henoch‐Schonlein purpura and association between selected microRNAs and inflammatory biomarkers
Therapeutic effect of inhaled budesonide (Pulmicort® Turbuhaler) on the inflammatory response to one‐lung ventilation
Butyrate as bioactive human milk protective component against food allergy
Effects of Different Blood Purification Methods on Serum Cytokine Levels and Prognosis in Patients With Acute Severe Organophosphorus Pesticide Poisoning
Effect of temperature maintenance by forced-air warming blankets of different temperatures on changes in inflammatory factors in children undergoing congenital hip dislocation surgery
DNMT1-mediated Foxp3 gene promoter hypermethylation involved in immune dysfunction caused by arsenic in human lymphocytes
Evaluation of pro-oxidant-antioxidant balance (PAB) and its association with inflammatory cytokines in polycystic ovary syndrome (PCOS)
Role of Regulatory T Cells in Tubercular Uveitis
Th17/Treg-related cytokine imbalance in sulfur mustard exposed and stable chronic obstructive pulmonary (COPD) patients: correlation with disease activity
CXCL4 promoted the production of CD4+CD25+FOXP3+treg cells in mouse sepsis model through regulating STAT5/FOXP3 pathway
The Impact of Pre-Cooling and CoQ10 Supplementation on Mediators of Inflammatory Cytokines in Elite Swimmers
Clinical features and monocyte/macrophage subsets characterization in granulomatous vs non-granulomatous Crohn’s disease
Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction
Randomized controlled trial on the influence of dietary intervention on epigenetic mechanisms in children with cow’s milk allergy: the EPICMA study
Altered gut microbiota after traumatic splenectomy is associated with endotoxemia
Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment
Antibacterial Fusion Protein BPI21/LL-37 Modification Enhances the Therapeutic Efficacy of hUC-MSCs in Sepsis
Serum Caspase-1 levels in women with polycystic ovary syndrome
JSH-23 prevents depressive-like behaviors in mice subjected to chronic mild stress: Effects on inflammation and antioxidant defense in the hippocampus
Necrostatin-1 Attenuates Renal Ischemia and Reperfusion Injury via Meditation of HIF-1α/mir-26a/TRPC6/PARP1 Signaling
Chronic obstructive pulmonary disease is characterized by reduced levels and defective suppressive function of regulatory B cells in peripheral blood
Lipopolysaccharide enhances human herpesvirus 1 replication and IL-6 release in epithelial cells
Enhanced immune responses in pigs by DNA vaccine coexpressing GP3 and GP5 of European type porcine reproductive and respiratory syndrome virus
Effect of preoperative inhaled budesonide on pulmonary injury after cardiopulmonary bypass: A randomized pilot study
Penehyclidine hydrochloride preserves the intestinal barrier function in patients undergoing cardiopulmonary bypass
Influence of Rotary Instrumentation with Continuous Irrigation on Pain and Neuropeptide Release Levels: A Randomized Clinical Trial
The role of human herpesvirus-6 and inflammatory markers in the pathogenesis of multiple sclerosis
The protective effect of Helicobacter Pylori infection on the susceptibility of multiple sclerosis
Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression
The influence of interferon-β supplemented human dendritic cells on BCG immunogenicity
Glycemic, inflammatory and oxidative stress responses to different high-intensity training protocols in type 1 diabetes: A randomized clinical trial
Effects of magnesium isoglycyrrhizinate on AST, ALT, and serum levels of Th1 cytokines in patients with allo-HSCT
Atorvastatin calcium in combination with methylprednisolone for the treatment of multiple sclerosis relapse
“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model
Genetic variations of IL-10: Identification of novel variations and evaluation of the impact of the SNPs/haplotype in the promoter region with the progression of Oral Squamous Cell Carcinoma in Indian population
Inflammatory cytokines and oxidative stress biomarkers in irritable bowel syndrome: Association with digestive symptoms and quality of life
Determination of cytokine levels in multiple sclerosis patients and their relevance with patients response to Cinnovex
Bufalin targets the SRC-3/MIF pathway in chemoresistant cells to regulate M2 macrophage polarization in colorectal cancer
Programmable immune activating electrospun fibers for skin regeneration
Elevated serum neopterin levels in children with functional constipation: association with systemic proinflammatory cytokines
The Association of Pre-diagnostic Inflammatory Markers and Adipokines and the Risk of Non-Hodgkin Lymphoma Development in Egypt
Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes
Shenqi Fuzheng Injection Ameliorates Radiation-induced Brain Injury
Interleukin-10 is expressed in HepG2.2.15 cells and regulated by STAT1 pathway
Regulation of ω-3 fish oil emulsion on the SIRS during the initial stage of severe acute pancreatitis
Comparison of inflammatory cytokines levels in the aqueous humor with diabetic retinopathy
Sex-Related Differences in Clinical Symptoms, Quality of Life, and Biochemical Factors in Irritable Bowel Syndrome
Elevated level of peripheral CD8 + CD28 − T lymphocytes are an independent predictor of progression-free survival in patients with metastatic breast cancer during the course of chemotherapy
Evaluation of pharmacokinetics and acute anti‐inflammatory potential of two oral cannabidiol preparations in healthy adults
A recombinant protein rLZ‐8, originally extracted from Ganoderma lucidum, ameliorates OVA‐induced lung inflammation by regulating Th17/Treg balance
A Human 3D In Vitro Model to Assess the Relationship Between Osteoporosis and Dissemination to Bone of Breast Cancer Tumor Cells
Biological and proteomic characterization of a composite mesh for abdominal wall hernia treatment: Reference Study
Singh A,Das D,Kurra S,Arava S,Gupta S,Sharma A.Dendritic cells and their associated pro-inflammatory cytokines augment to the inflammatory milieu in vitiligo skin.Cytokine.2021 Jun 5:155598.doi:10.1016/j.cyto.2021.155598.Epub ahead of print.PMID:34103210.
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:DENDRITIC CELL, DILUTION:NA
Yu D,Zhao Y,Wang H,Kong D,Jin W,Hu Y,Qin Y,Zhang B,Li X,Hao J,Li G,Wang H.IL-1β pre-stimulation enhances the therapeutic effects of endometrial regenerative cells on experimental colitis. Stem Cell Res Ther.2021 Jun 5;12(1):324.doi:10.1186/s13287-021-02392-9.PMID:34090510.
Species: Human,Mouse
EK0416 usage in article: APP:ELISA, SAMPLE:ERCS, DILUTION:NA
Zedan MM,Zedan MM,El-Said AM,Badran AM,Wahba Y.Evaluation of serum levels of interleukin-10 and transforming growth factor-β1 among Egyptian children with wheezy asthma phenotype.Egypt J Chest Dis Tuberc 2021;70:208-14
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Chen J,Wang H,Jia L,He J,Li Y,Liu H,Wu R,Qiu Y,Zhan Y,Yuan Z,Cao Y,Li W,Xu K,Yin P.Bufalin targets the SRC-3/MIF pathway in chemoresistant cells to regulate M2 macrophage polarization in colorectal cancer.Cancer Lett.2021 May 14:S0304-3835(21)00223-8.doi:10.1016/j.canlet.2021.05.008.Epub ahead of print.PMID:34000344.
Species: Human,Mouse
EK0416 usage in article: APP:ELISA, SAMPLE:CRC CELL, DILUTION:NA
Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
Species: Human,Mouse
EK0416 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA
Guo L,Jiang B,Li D,Xiao X. Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor.Drug Des Devel Ther.2021 Feb 16;15:589-600.doi:10. 2147/DDDT.S294009.PMID:33623368;PMCID
Species: Rat
EK0416 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
The effectiveness of olmesartan on inflammation at cardiopulmonary bypass. Ibrahim Murat Ozguler,Latif Ustunel
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Casciaro F,Zia S,Forcato M,Zavatti M,Beretti F,Bertucci E,Zattoni A,Reschiglian P,Alviano F,Bonsi L,Follo MY,Demaria M,Roda B,Maraldi T.Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations.Cells.2021 Jan 15;10(1):158.doi:
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:HAFSCS, DILUTION:NA
Liu H,Chen W,Zhao B,Quan W,Zhang Y,Zhou Y,Wan Z,Zhang X,Xue G,Li J,Luo S,Wang J,Liu Y,Zhen M,Zhao Y.Autologous bionic tissue for inguinal hernia repair.J Biomed Mater Res A.2020 Jun; 108(6):1351-1368.doi:10.1002/jbm.a.36907.Epub 2020 Mar 2.PMID:32090432.
Species: New Zealand white Rabbit
EK0416 usage in article: APP:ELISA, SAMPLE:MSCS, DILUTION:NA
Das D, Singh A, Antil PS, Sharma D, Arava S, Khandpur S, Sharma A. Distorted frequency of dendritic cells and their associated stimulatory and inhibitory markers augment the pathogenesis of pemphigus vulgaris. Immunol Res. 2020 Nov 12. doi: 10.1007/s12026
Species: Human
EK0416 usage in article: APP:ELISA, SAMPLE:PBMCS, DILUTION:NA
Duan, Y., Zheng, H., Li, Z., Yao, Y., Ding, J., Wang, X., ... & Zheng, X. (2020). Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis. Biomaterials, 120012.
The Impact of Pre-Cooling and CoQ 10 Supplementation on Mediators of Inflammatory Cytokines in Elite Swimmers
Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis
Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis
Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in vitro stimulation with ?-cell autoantigens
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma
Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome
Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica
Evaluation of Interleukin-10 Levels in Patients Diagnosed with Chronic Hepatitis
Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells
Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155
Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages
Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery
Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients
Immunomodulatory effects of total intravenous and balanced inhalation anesthesia in patients with bladder cancer undergoing elective radical Cystectomy: preliminary results
HER2-specific T lymphocytes kill both trastuzumab-resistant and trastuzumab-sensitive breast cell lines in vitro
Dang, W., Xie, W. & Zhou, J. (2017). Expression of PYCARD Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Primary Gout Patients with Different Chinese Medicine Syndromes. Chinese Journal of Integrative Medicine, 1-8. Advance ...
He, S., Le, N.A., Frediani, J.K., Winterberg, P.D., Jin, R., Liverman, R.,..., & Vos, M.B. (2017). Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. ...
Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.
Luo H,, Wang X,, Wang J,, Chen C,, Wang N,, Xu Z,, Chen S,, Zeng C,. Cardiovasc Diabetol. 2015 Jun 10;14:76. Doi: 10.1186/S12933-015-0239-7. Chronic Nf-??b Blockade Improves Renal Angiotensin Ii Type 1 Receptor Functions And Reduces Blood Pressure...
Wang T, Zhou Yt, Chen Xn, Zhu Ax, Wu Bh. World J Gastroenterol. 2014 Jul 28;20(28):9519-27. Doi: 10.3748/Wjg.V20.I28.9519. Remote Ischemic Postconditioning Protects Against Gastric Mucosal Lesions In Rats.
Zhang X, Liu F, Liu H, Cheng H, Wang W, Wen Q, Wang Y. Inflamm Res. 2011 Jun;60(6):569-75. Doi: 10.1007/S00011-010-0305-2. Epub 2011 Jan 19. Urinary Trypsin Inhibitor Attenuates Lipopolysaccharide-Induced Acute Lung Injury By Blocking The Activati...
Chen T, Liu W, Chao X, Zhang L, Qu Y, Huo J, Fei Z. Brain Res Bull. 2011 Feb 1;84(2):163-8. Doi: 10.1016/J.Brainresbull.2010.11.015. Epub 2010 Dec 4. Salvianolic Acid B Attenuates Brain Damage And Inflammation After Traumatic Brain Injury In Mice.
Li Xj, Liu Dp, Chen Hl, Pan Xh, Kong Qy, Pang Qf. Int Immunopharmacol. 2012 Feb;12(2):460-4. Doi: 10.1016/J.Intimp.2012.01.001. Epub 2012 Jan 15. Lactoferrin Protects Against Lipopolysaccharide-Induced Acute Lung Injury In Mice.
Xia J, Zhou Xl, Zhao Y, Zhu Yq, Jiang S, Ni Sz. Inflammation. 2013 Dec;36(6):1431-9. Doi: 10.1007/S10753-013-9683-2. Roles Of Lipoxin A4 In Preventing Paracetamol-Induced Acute Hepatic Injury In A Rabbit Model.
Tang Y, Liao Y, Kawaguchi-Sakita N, Raut V, Fakhrejahani E, Qian N, Toi M. J Hepatobiliary Pancreat Sci. 2011 Jul;18(4):551-8. Doi: 10.1007/S00534-010-0368-Z. Sinisan, A Traditional Chinese Medicine, Attenuates Experimental Chronic Pancreatitis In...
Chen W, Qi J, Feng F, Wang Md, Bao G, Wang T, Xiang M, Xie Wf. Neurochem Int. 2014 Mar;68:28-37. Doi: 10.1016/J.Neuint.2014.01.015. Epub 2014 Feb 12. Neuroprotective Effect Of Allicin Against Traumatic Brain Injury Via Akt/Endothelial Nitric Oxide...
Ye Xh, Wu Y, Guo Pp, Wang J, Yuan Sy, Shang Y, Yao Sl. Brain Res. 2010 Apr 6;1323:174-83. Doi: 10.1016/J.Brainres.2010.01.079. Epub 2010 Feb 4. Lipoxin A4 Analogue Protects Brain And Reduces Inflammation In A Rat Model Of Focal Cerebral Ischemia R...
Jin Y, Wan Y, Chen G, Chen L, Zhang Mq, Deng L, Zhang Jc, Xiong Xz, Xin Jb. Plos One. 2014 Oct 16;9(10):E111044. Doi: 10.1371/Journal.Pone.0111044. Ecollection 2014. Treg/Il-17 Ratio And Treg Differentiation In Patients With Copd.
Sereshki N, Gharagozloo M, Ostadi V, Ghahiri A, Roghaei Ma, Mehrabian F, Andalib Aa, Hassanzadeh A, Hosseini H, Rezaei A. Int J Fertil Steril. 2014 Apr;8(1):59-66. Epub 2014 Mar 9. Variations In T-Helper 17 And Regulatory T Cells During The Menstr...
Hualin C, Wenli X, Dapeng L, Xijing L, Xiuhua P, Qingfeng P. Inflammation. 2012 Jun;35(3):1087-93. Doi: 10.1007/S10753-011-9415-4. The Anti-Inflammatory Mechanism Of Heme Oxygenase-1 Induced By Hemin In Primary Rat Alveolar Macrophages.
Zhou X, Cao Y, Ao G, Hu L, Liu H, Wu J, Wang X, Jin M, Zheng S, Zhen X, Alkayed Nj, Jia J, Cheng J. Antioxid Redox Signal. 2014 Oct 20;21(12):1741-58. Doi: 10.1089/Ars.2013.5587. Epub 2014 Mar 13. Camkk??-Dependent Activation Of Amp-Activated Prot...
Sun Hx, Chen Lq, Zhang J, Chen Fy. J Ethnopharmacol. 2014 May 14;153(3):831-9. Doi: 10.1016/J.Jep.2014.03.052. Epub 2014 Mar 29. Anti-Tumor And Immunomodulatory Activity Of Peptide Fraction From The Larvae Of Musca Domestica.
Ruan Z, Liu S, Zhou Y, Mi S, Liu G, Wu X, Yao K, Assaad H, Deng Z, Hou Y, Wu G, Yin Y. Plos One. 2014 Jun 2;9(6):E97815. Doi: 10.1371/Journal.Pone.0097815. Ecollection 2014. Chlorogenic Acid Decreases Intestinal Permeability And Increases Expressi...
Meng A, Wang B, Zhang X, Qi N, Liu D, Wu J. Inflammation. 2015;38(3):1260-6. Doi: 10.1007/S10753-014-0093-X. Additive Suppression Of Lps-Induced Il-10 And Tnf-?? By Pre-Treatment Of Dexamethasone And Sb203580 In A Murine Alveolar Macrophage Cell L...
Chen W, Guo Y, Yang W, Zheng P, Zeng J, Tong W. Exp Brain Res. 2015 Oct;233(10):2823-31. Doi: 10.1007/S00221-015-4352-3. Epub 2015 Jun 13. Protective Effect Of Ginsenoside Rb1 On Integrity Of Blood-Brain Barrier Following Cerebral Ischemia.
Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...
Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...
Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.
Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...
Yin Y, Qin T, Yu Q, Yang Q. Amino Acids. 2014 Jul;46(7):1763-74. Doi: 10.1007/S00726-014-1735-X. Epub 2014 Apr 8. Bursopentin (Bp5) From Chicken Bursa Of Fabricius Attenuates The Immune Function Of Dendritic Cells.
Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...
Chen X, Zhao C, Li X, Wang T, Li Y, Cao C, Ding Y, Dong M, Finci L, Wang Jh, Li X, Liu L. Nat Chem Biol. 2015 Jan;11(1):19-25. Doi: 10.1038/Nchembio.1657. Epub 2014 Nov 10. Terazosin Activates Pgk1 And Hsp90 To Promote Stress Resistance.
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...
Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.
Zhang Y, Zhang S, Li H, Huang M, Xu W, Chu K, Chen L, Chen X. Mol Med Rep. 2015 Jul;12(1):988-94. Doi: 10.3892/Mmr.2015.3515. Epub 2015 Mar 19. Ameliorative Effects Of Gualou Guizhi Decoction On Inflammation In Focal Cerebral Ischemic-Reperfusion ...
Shi Jq, Chen J, Wang Br, Zhu Yw, Xu Y, Wang J, Xiao H, Shi Jp, Zhang Yd, Xu J. Peptides. 2011 Aug;32(8):1617-25. Doi: 10.1016/J.Peptides.2011.06.013. Epub 2011 Jun 21. Short Amyloid-Beta Immunogens Show Strong Immunogenicity And Avoid Stimulating ...
Zhang H, Xia X, Han F, Jiang Q, Rong Y, Song D, Wang Y. Mol Pharm. 2015 May 4;12(5):1648-61. Doi: 10.1021/Acs.Molpharmaceut.5B00069. Epub 2015 Apr 2. Cathelicidin-Bf, A Novel Antimicrobial Peptide From Bungarus Fasciatus, Attenuates Disease In A D...
Wang Sb, Deng Yq, Ren J, Xiao Bk, Chen Z, Tao Zz. Scand J Immunol. 2013 Dec;78(6):507-15. Doi: 10.1111/Sji.12118. Lactoferrin Administration Into The Nostril Alleviates Murine Allergic Rhinitis And Its Mechanisms.
Ozkara S, Keles E, Ilhan N, Gungor H, Kaygusuz I, Alpay Hc. Eur Arch Otorhinolaryngol. 2012 Dec;269(12):2519-24. Doi: 10.1007/S00405-012-1967-X. Epub 2012 Feb 23. The Relationship Between Th1/Th2 Balance And 1??,25-Dihydroxyvitamin D
Berni Canani R, Paparo L, Nocerino R, Cosenza L, Pezzella V, Di Costanzo M, Capasso M, Del Monaco V, D'Argenio V, Greco L, Salvatore F. Clin Epigenetics. 2015 Mar 31;7(1):38. Doi: 10.1186/S13148-015-0070-8. Ecollection 2015. Differences In Dna Met...
Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model.
Ou J, Shi W, Xu Y, Tao Z. Ann Allergy Asthma Immunol. 2014 Dec;113(6):658-665.E1. Doi: 10.1016/J.Anai.2014.08.015. Epub 2014 Sep 17. Intranasal Immunization With Dna Vaccine Coexpressing Der P 1 And Ubiquitin In An Allergic Rhinitis Mouse Model.
Jin Q, Cheng J, Liu Y, Wu J, Wang X, Wei S, Zhou X, Qin Z, Jia J, Zhen X. Brain Behav Immun. 2014 Aug;40:131-42. Doi: 10.1016/J.Bbi.2014.03.003. Epub 2014 Mar 12. Improvement Of Functional Recovery By Chronic Metformin Treatment Is Associated With...
Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...
Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro.
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????
Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...
Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...
Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human IL-10/Interleukin-10 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Human IL-10/Interleukin-10 ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
12 Customer Q&As for Human IL-10/Interleukin-10 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated IL-10 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-10 antigen?
Verified Customer
Verified customer
Asked: 2020-12-08
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-12-08
Question
Q: can you recommend the dilution ratio of serum samples for detection of IL-10 in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Y. Li
Verified customer
Asked: 2020-03-09
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-09
Question
Q: Can IL-10 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Puri
Verified customer
Asked: 2019-07-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-07-23
Question
Have you tested EK0410 and EK0416 in tissue homogenates and Cell culture supernates?
Verified customer
Asked: 2019-03-15
Answer
The Human IL-6 EK0410 Elisa Kit and Human IL-10 EK0416 Elisa Kit have not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-03-18
Question
Q: is it okay to use EDTA plasma as samples in Human IL-10 Picokine® ELISA Kit (Catalog # EK0416)?
H. Smith
Verified customer
Asked: 2018-10-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-10 causing degradation of its protein structure. IL-10 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-10 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-01
Question
My sample type is cell supernates. I would like to know what ratio should I use to dilute my samples during using your product EK0416 and EK0525. I couldn't find any reference from the product page.
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
For untreated cell supernatants, the suggested dilution ratio is 1:1. For treated cell supernatants, concentrations of the samples can vary greatly. We suggest the customer run pilot tests for different group samples to figure out the optimal dilution ratio.
Boster Scientific Support
Answered: 2018-08-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-08-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-05
Question
I am looking for IL 10 that works both with human and mouse is the EK0416 antibody kit will cross react with human and mouse?
Verified Customer
Verified customer
Asked: 2018-06-21
Answer
The EK0416 antibody kit did not show any cross-reactivity with mouse samples, therefore, this kit is not expected to cross react.
Boster Scientific Support
Answered: 2018-06-21
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-06-16
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-08
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
M. Walker
Verified customer
Asked: 2017-11-25
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-11-25
Question
Q: how can I thaw whole blood sample for IL-10 ELISA after freezing?
N. Moore
Verified customer
Asked: 2017-10-22
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-10 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-10-22
