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Human IL-10 ELISA Kit PicoKine™

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-10. 96wells/kit, with removable strips. Cited in 64 publication(s).

Publications (64)
Q&As (2)
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Human IL-10 PicoKine ELISA Kit standard curve

Human IL-10 ELISA Kit PicoKine™ Info At A Glance

SKU:	EK0416
Size:	96wells/kit, with removable strips.
Reactivity:	Human
Sample Type:	cell culture supernates, serum and plasma( heparin, EDTA, citrate).
Sample Volume:	100μl per well
Sensitivity:	<0.5pg/ml
Assay Range:	7.8pg/ml-500pg/ml(cellculturesupernates)3.4pg/ml-250pg/ml(humanserum,plasma)

Product Info

Overview

Product Name	Human IL-10 ELISA Kit PicoKine™ See all IL-10 primary antibodies, ELISA kits and proteins
SKU/Catalog Number	EK0416
Storage & Handling	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Description	Detect Human IL10 with <0.5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL10 tissue specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Cite This Product	Human IL-10 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0416)
ELISA Validation	ELISA Validation Information

Product Specs

Reactivity/Species	Human
Applications	ELISA Our Boster Guarantee covers the use of this product in the above tested applications. *For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
Sample Type	cell culture supernates, serum and plasma( heparin, EDTA, citrate).
Sensitivity	<0.5pg/ml
Assay Range	7.8pg/ml-500pg/ml(cellculturesupernates)3.4pg/ml-250pg/ml(humanserum,plasma)
Immunogen/Standard	Expression system for standard: E.coli; Immunogen sequence: S19-N178
Antibody Clonalities	Capture antibody\|Detection antibody monoclonal antibody from mouse\|monoclonal antibody from mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Pack Size	96wells/kit, with removable strips.

Protein Target Info

Gene/Protein Basic Information For IL10 (Source: Uniprot.org, NCBI)

Uniprot Id	P22301
NCBI Gene Id	3586
Species Of This Entry	Human
Gene Name	IL10
Protein Name	Interleukin-10
Superfamily	IL-10 family
Alternative Names	IL-10\|CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
Molecular Weight	20517
Post Tranlational Modifications	Phosphorylation; Methylation; Acetylation; Cleavage; Oxidation; Demethylation; Glycosylation; Citrullination; Dephosphorylation; Nitration; Nitrosylation; Ubiquitination; Iodination; Reduction; Deacetylation
Subcellular Localization	Secreted.
Gene Location	1q32.1, on Chromasome 1, gene sequence: NC_000001.11 (206767602..206772494, complement)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

Ontology For IL10 (Source: Uniprot.org, NCBI)

Protein Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Tissue Specificity	Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Interacting Proteins	IL10RA; IL10RB
Related Diseases	Inflammation; Malignant Neoplasms; Autoimmune Reaction; Inflammatory Response; Neoplasms; Colitis; Autoimmune Diseases; Arthritis; Infective Disorder; Inflammatory Bowel Diseases; Lymphoma; Rheumatoid Arthritis; Intestinal Diseases; Asthma; Sclerosis; Lupus Erythematosus, Systemic; Hepatitis; Diabetes Mellitus; Hiv Infections; Multiple Sclerosis
Related Pathways	Immune Response; Secretion; Pathogenesis; Cytokine Production; Inflammatory Response; Cytokine Secretion; Cell Proliferation; Cell Activation; Innate Immune Response; Cell Death; T Cell Proliferation; T Cell Activation; Hypersensitivity; Sensitization; Lymphocyte Proliferation; Cell Cycle; Macrophage Activation; Phagocytosis; Angiogenesis; Methylation
Background	Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
Scientific References	PMID: 1847510 by Vieira P., et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI. PMID: 9405662 by Gesser B., et al. Identification of functional domains on human interleukin 10.

Kit Components And Assay QC Data Details Of Human IL-10 ELISA Kit PicoKine™

*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.

Kit Components

Catalog number	Description	Quantity
EK0416-CAP	96-well plate precoated with anti-Human IL-10 antibody	1
EK0416-ST	lyophilized recombinant Human IL-10 standard	10ng/tube
EK0416-DA	biotinylated anti-Human IL-10 antibody	130ul
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	130ul
AR1106-1	sample diluent buffer	30ml
AR1106-2	antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Materials Required But Not Provided

Microplate reader in standard size.
Automated plate washer.
Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
Clean tubes and Eppendorf tubes.
Washing buffer (neutral PBS or TBS).
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained From Human IL-10 ELISA Kit PicoKine™

This data is generated from a recent batch of EK0416 Human IL-10 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.070	0.151	0.187	0.295	0.538	0.858	1.622	2.281

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-10 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	16	53	113	15	54	122
Standard deviation	1.02	2.12	7.34	1.24	3.02	9.88
CV(%)	6.4%	4%	6.5%	8.3%	5.6%	8.1%

Reproducibility

We measured the performance consistency of Human IL-10 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	16	17	15	15	15	0.82	5.4%
Sample 2	53	45	47	48	48	2.94	6.1%
Sample 3	113	123	106	104	111	7.43	6.6%

*number of samples for each test n=16.

Specific Protocols

Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.

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Publications citing Boster Bio's EK0416 Human IL-10 ELISA Kit PicoKine™

Total number of citations: 64

Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...	PubMed ID 23423691
Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...	PubMed ID 22913292
Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...	PubMed ID 22464914
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????	PubMed ID 25428340
Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro.	PubMed ID 20609400
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...	PubMed ID 25744647
Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...	PubMed ID 24368037
Jin Q, Cheng J, Liu Y, Wu J, Wang X, Wei S, Zhou X, Qin Z, Jia J, Zhen X. Brain Behav Immun. 2014 Aug;40:131-42. Doi: 10.1016/J.Bbi.2014.03.003. Epub 2014 Mar 12. Improvement Of Functional Recovery By Chronic Metformin Treatment Is Associated With...	PubMed ID 24632338
Ou J, Shi W, Xu Y, Tao Z. Ann Allergy Asthma Immunol. 2014 Dec;113(6):658-665.E1. Doi: 10.1016/J.Anai.2014.08.015. Epub 2014 Sep 17. Intranasal Immunization With Dna Vaccine Coexpressing Der P 1 And Ubiquitin In An Allergic Rhinitis Mouse Model.	PubMed ID 25240330
Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model.	PubMed ID 24738018
Berni Canani R, Paparo L, Nocerino R, Cosenza L, Pezzella V, Di Costanzo M, Capasso M, Del Monaco V, D'Argenio V, Greco L, Salvatore F. Clin Epigenetics. 2015 Mar 31;7(1):38. Doi: 10.1186/S13148-015-0070-8. Ecollection 2015. Differences In Dna Met...	PubMed ID 25859290
Ozkara S, Keles E, Ilhan N, Gungor H, Kaygusuz I, Alpay Hc. Eur Arch Otorhinolaryngol. 2012 Dec;269(12):2519-24. Doi: 10.1007/S00405-012-1967-X. Epub 2012 Feb 23. The Relationship Between Th1/Th2 Balance And 1??,25-Dihydroxyvitamin D	PubMed ID 22358381
Wang Sb, Deng Yq, Ren J, Xiao Bk, Chen Z, Tao Zz. Scand J Immunol. 2013 Dec;78(6):507-15. Doi: 10.1111/Sji.12118. Lactoferrin Administration Into The Nostril Alleviates Murine Allergic Rhinitis And Its Mechanisms.	PubMed ID 24111738
Zhang H, Xia X, Han F, Jiang Q, Rong Y, Song D, Wang Y. Mol Pharm. 2015 May 4;12(5):1648-61. Doi: 10.1021/Acs.Molpharmaceut.5B00069. Epub 2015 Apr 2. Cathelicidin-Bf, A Novel Antimicrobial Peptide From Bungarus Fasciatus, Attenuates Disease In A D...	PubMed ID 25807257
Shi Jq, Chen J, Wang Br, Zhu Yw, Xu Y, Wang J, Xiao H, Shi Jp, Zhang Yd, Xu J. Peptides. 2011 Aug;32(8):1617-25. Doi: 10.1016/J.Peptides.2011.06.013. Epub 2011 Jun 21. Short Amyloid-Beta Immunogens Show Strong Immunogenicity And Avoid Stimulating ...	PubMed ID 21722679
Zhang Y, Zhang S, Li H, Huang M, Xu W, Chu K, Chen L, Chen X. Mol Med Rep. 2015 Jul;12(1):988-94. Doi: 10.3892/Mmr.2015.3515. Epub 2015 Mar 19. Ameliorative Effects Of Gualou Guizhi Decoction On Inflammation In Focal Cerebral Ischemic-Reperfusion ...	PubMed ID 25815894
Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.	PubMed ID 25626689
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...	PubMed ID 26022197
Chen X, Zhao C, Li X, Wang T, Li Y, Cao C, Ding Y, Dong M, Finci L, Wang Jh, Li X, Liu L. Nat Chem Biol. 2015 Jan;11(1):19-25. Doi: 10.1038/Nchembio.1657. Epub 2014 Nov 10. Terazosin Activates Pgk1 And Hsp90 To Promote Stress Resistance.	PubMed ID 25383758
Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...	PubMed ID 25725585
Yin Y, Qin T, Yu Q, Yang Q. Amino Acids. 2014 Jul;46(7):1763-74. Doi: 10.1007/S00726-014-1735-X. Epub 2014 Apr 8. Bursopentin (Bp5) From Chicken Bursa Of Fabricius Attenuates The Immune Function Of Dendritic Cells.	PubMed ID 24710706
Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...	PubMed ID 23746956
Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.	PubMed ID 24955162
Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...	PubMed ID 23022502
Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...	PubMed ID 22863994
Chen W, Guo Y, Yang W, Zheng P, Zeng J, Tong W. Exp Brain Res. 2015 Oct;233(10):2823-31. Doi: 10.1007/S00221-015-4352-3. Epub 2015 Jun 13. Protective Effect Of Ginsenoside Rb1 On Integrity Of Blood-Brain Barrier Following Cerebral Ischemia.	PubMed ID 26070903
Meng A, Wang B, Zhang X, Qi N, Liu D, Wu J. Inflammation. 2015;38(3):1260-6. Doi: 10.1007/S10753-014-0093-X. Additive Suppression Of Lps-Induced Il-10 And Tnf-?? By Pre-Treatment Of Dexamethasone And Sb203580 In A Murine Alveolar Macrophage Cell L...	PubMed ID 25563207
Ruan Z, Liu S, Zhou Y, Mi S, Liu G, Wu X, Yao K, Assaad H, Deng Z, Hou Y, Wu G, Yin Y. Plos One. 2014 Jun 2;9(6):E97815. Doi: 10.1371/Journal.Pone.0097815. Ecollection 2014. Chlorogenic Acid Decreases Intestinal Permeability And Increases Expressi...	PubMed ID 24887396
Sun Hx, Chen Lq, Zhang J, Chen Fy. J Ethnopharmacol. 2014 May 14;153(3):831-9. Doi: 10.1016/J.Jep.2014.03.052. Epub 2014 Mar 29. Anti-Tumor And Immunomodulatory Activity Of Peptide Fraction From The Larvae Of Musca Domestica.	PubMed ID 24690776
Zhou X, Cao Y, Ao G, Hu L, Liu H, Wu J, Wang X, Jin M, Zheng S, Zhen X, Alkayed Nj, Jia J, Cheng J. Antioxid Redox Signal. 2014 Oct 20;21(12):1741-58. Doi: 10.1089/Ars.2013.5587. Epub 2014 Mar 13. Camkk??-Dependent Activation Of Amp-Activated Prot...	PubMed ID 24624937
Hualin C, Wenli X, Dapeng L, Xijing L, Xiuhua P, Qingfeng P. Inflammation. 2012 Jun;35(3):1087-93. Doi: 10.1007/S10753-011-9415-4. The Anti-Inflammatory Mechanism Of Heme Oxygenase-1 Induced By Hemin In Primary Rat Alveolar Macrophages.	PubMed ID 22160840
Sereshki N, Gharagozloo M, Ostadi V, Ghahiri A, Roghaei Ma, Mehrabian F, Andalib Aa, Hassanzadeh A, Hosseini H, Rezaei A. Int J Fertil Steril. 2014 Apr;8(1):59-66. Epub 2014 Mar 9. Variations In T-Helper 17 And Regulatory T Cells During The Menstr...	PubMed ID 24695956
Jin Y, Wan Y, Chen G, Chen L, Zhang Mq, Deng L, Zhang Jc, Xiong Xz, Xin Jb. Plos One. 2014 Oct 16;9(10):E111044. Doi: 10.1371/Journal.Pone.0111044. Ecollection 2014. Treg/Il-17 Ratio And Treg Differentiation In Patients With Copd.	PubMed ID 25329073
Ye Xh, Wu Y, Guo Pp, Wang J, Yuan Sy, Shang Y, Yao Sl. Brain Res. 2010 Apr 6;1323:174-83. Doi: 10.1016/J.Brainres.2010.01.079. Epub 2010 Feb 4. Lipoxin A4 Analogue Protects Brain And Reduces Inflammation In A Rat Model Of Focal Cerebral Ischemia R...	PubMed ID 20138164
Chen W, Qi J, Feng F, Wang Md, Bao G, Wang T, Xiang M, Xie Wf. Neurochem Int. 2014 Mar;68:28-37. Doi: 10.1016/J.Neuint.2014.01.015. Epub 2014 Feb 12. Neuroprotective Effect Of Allicin Against Traumatic Brain Injury Via Akt/Endothelial Nitric Oxide...	PubMed ID 24530793
Tang Y, Liao Y, Kawaguchi-Sakita N, Raut V, Fakhrejahani E, Qian N, Toi M. J Hepatobiliary Pancreat Sci. 2011 Jul;18(4):551-8. Doi: 10.1007/S00534-010-0368-Z. Sinisan, A Traditional Chinese Medicine, Attenuates Experimental Chronic Pancreatitis In...	PubMed ID 21234610
Xia J, Zhou Xl, Zhao Y, Zhu Yq, Jiang S, Ni Sz. Inflammation. 2013 Dec;36(6):1431-9. Doi: 10.1007/S10753-013-9683-2. Roles Of Lipoxin A4 In Preventing Paracetamol-Induced Acute Hepatic Injury In A Rabbit Model.	PubMed ID 23851615
Li Xj, Liu Dp, Chen Hl, Pan Xh, Kong Qy, Pang Qf. Int Immunopharmacol. 2012 Feb;12(2):460-4. Doi: 10.1016/J.Intimp.2012.01.001. Epub 2012 Jan 15. Lactoferrin Protects Against Lipopolysaccharide-Induced Acute Lung Injury In Mice.	PubMed ID 22251871
Chen T, Liu W, Chao X, Zhang L, Qu Y, Huo J, Fei Z. Brain Res Bull. 2011 Feb 1;84(2):163-8. Doi: 10.1016/J.Brainresbull.2010.11.015. Epub 2010 Dec 4. Salvianolic Acid B Attenuates Brain Damage And Inflammation After Traumatic Brain Injury In Mice.	PubMed ID 21134421
Zhang X, Liu F, Liu H, Cheng H, Wang W, Wen Q, Wang Y. Inflamm Res. 2011 Jun;60(6):569-75. Doi: 10.1007/S00011-010-0305-2. Epub 2011 Jan 19. Urinary Trypsin Inhibitor Attenuates Lipopolysaccharide-Induced Acute Lung Injury By Blocking The Activati...	PubMed ID 21246393
Wang T, Zhou Yt, Chen Xn, Zhu Ax, Wu Bh. World J Gastroenterol. 2014 Jul 28;20(28):9519-27. Doi: 10.3748/Wjg.V20.I28.9519. Remote Ischemic Postconditioning Protects Against Gastric Mucosal Lesions In Rats.	PubMed ID 25071347
Luo H,, Wang X,, Wang J,, Chen C,, Wang N,, Xu Z,, Chen S,, Zeng C,. Cardiovasc Diabetol. 2015 Jun 10;14:76. Doi: 10.1186/S12933-015-0239-7. Chronic Nf-??b Blockade Improves Renal Angiotensin Ii Type 1 Receptor Functions And Reduces Blood Pressure...	PubMed ID 26055622
Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.	PubMed ID 21246001
He, S., Le, N.A., Frediani, J.K., Winterberg, P.D., Jin, R., Liverman, R.,..., & Vos, M.B. (2017). Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. ...	PubMed ID 28718192
Dang, W., Xie, W. & Zhou, J. (2017). Expression of PYCARD Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Primary Gout Patients with Different Chinese Medicine Syndromes. Chinese Journal of Integrative Medicine, 1-8. Advance ...	PubMed ID 29086221
HER2-specific T lymphocytes kill both trastuzumab-resistant and trastuzumab-sensitive breast cell lines in vitro	PubMed ID 23357961
Immunomodulatory effects of total intravenous and balanced inhalation anesthesia in patients with bladder cancer undergoing elective radical Cystectomy: preliminary results	PubMed ID 23374147
Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients	PubMed ID 24250536
Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery	PubMed ID 25663985
Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages	PubMed ID 25861639
Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155	PubMed ID 26191144
Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells	PubMed ID 26295043
Evaluation of Interleukin-10 Levels in Patients Diagnosed with Chronic Hepatitis	PubMed ID 26360676
Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica	PubMed ID 26630909
Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome	PubMed ID 27168826
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma	PubMed ID 27494859
Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in vitro stimulation with ?-cell autoantigens	PubMed ID 29067979
Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis	PubMed ID 29805485
Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis	PubMed ID 26673286
The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility	PubMed ID 29914245
Increased Age-Related Cardiac Dysfunction in Bradykinin B2 Receptor%u2013Deficient Mice	PubMed ID 25452401
Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome	PubMed ID 29062341
Alterations of serum cytokine levels and their relation with inflammatory markers in candidemia	PubMed ID 25627661
The Impact of Pre-Cooling and CoQ 10 Supplementation on Mediators of Inflammatory Cytokines in Elite Swimmers	PubMed ID 31094229

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Customer Q&As

Questions and answers from customers related to EK0416 Human IL-10 ELISA Kit PicoKine™

2 Related Questions

Question

My sample type is cell supernates. I would like to know what ratio should I use to dilute my samples during using your product EK0416 and EK0525. I couldn't find any reference from the product page.

Verified Customer

Asked: 2018-08-08

Answer

For untreated cell supernatants, the suggested dilution ratio is 1:1. For treated cell supernatants, concentrations of the samples can vary greatly. We suggest the customer run pilot tests for different group samples to figure out the optimal dilution ratio.

Boster Scientific Support

Answered: 2018-08-08

Question

I am looking for IL 10 that works both with human and mouse is the EK0416 antibody kit will cross react with human and mouse?

Verified Customer

Asked: 2018-06-21

Answer

The EK0416 antibody kit did not show any cross-reactivity with mouse samples, therefore, this kit is not expected to cross react.

Boster Scientific Support

Answered: 2018-06-21

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EK0416

$369.00

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1 kit $369.00

2 kits pack $701.00

6 kits pack $1,992.00

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Human IL-10 ELISA Kit PicoKine™

Human IL-10 ELISA Kit PicoKine™ Info At A Glance

Overview

Product Specs

Gene/Protein Basic Information For IL10 (Source: Uniprot.org, NCBI)

Ontology For IL10 (Source: Uniprot.org, NCBI)

Kit Components And Assay QC Data Details Of Human IL-10 ELISA Kit PicoKine™

Reproducibility

Specific Protocols

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