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Product Info Summary
| SKU: | EK0539 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
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Product info
Product Name
Human VEGF ELISA Kit PicoKine®
SKU/Catalog Number
EK0539
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human VEGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human VEGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0539)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A27-R191
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0539 is reactive to VEGFA in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0539 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFA
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0539-CAP | Anti-Human VEGFA Pre-coated 96-well strip microplate | 1 |
| EK0539-ST | Human VEGFA Standard | 2 vials, 10 ng/tube |
| EK0539-DA | Human VEGFA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.020 | 0.055 | 0.113 | 0.189 | 0.345 | 0.589 | 1.106 | 1.913 |
Data Example Images
Click image to see more details
Human VEGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGFA, Dilution ratio of 1:1, concentration in serum is around 80pg/ml.
Some articles we found to cite concentrations of VEGFA in samples: 25374209 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human VEGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 72 | 270 | 946 | 76 | 254 | 955 |
| Standard deviation | 4.53 | 21.33 | 59.59 | 9.62 | 24.38 | 74.49 |
| CV (%) | 9.6% | 7.9% | 9.6% | 7.8% | 9.6% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VEGFA in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 72 | 80 | 82 | 71 | 76 | 4.81 | 6.3% |
| Sample 2 | 270 | 267 | 252 | 245 | 258 | 10.35 | 4% |
| Sample 3 | 946 | 897 | 1026 | 1037 | 976 | 57.79 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For VEGFA (Source: Uniprot.Org, NCBI)
Uniprot ID
P15692
Gene ID
7422
Gene Name
VEGFA
Full Name
Vascular endothelial growth factor A
Weight
27.042kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on VEGFA, check out the VEGFA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for VEGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human VEGF ELISA Kit PicoKine® (EK0539)
EK0539 has been cited in 79 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A simple electrochemical aptasensor for ultrasensitive protein detection using cyclic target-induced primer extension
Species: Human
Inducing a Transient Increase in Blood–Brain Barrier Permeability for Improved Liposomal Drug Therapy of Glioblastoma Multiforme
Species: Human
Modular Peptide Probe for Pre/Intra/Postoperative Therapeutic to Reduce Recurrence in Ovarian Cancer
Species: Mouse
Argueta LB,Niles JA,Sakamoto J,Liu X,Vega SP,Frank L,Paessler M,Cortiella J,Nichols JE.Platforms to test the Temporospatial capabilities of carrier Systems in Delivering Growth Factors to benefit vascular bioengineering.Nanomedicine.2021 Jun 17:102419.doi:10.1016/j.nano.2021.102419.Epub ahead of print.PMID:34147665.
Species: Porcine
EK0539 usage in article: APP:ELISA, SAMPLE:VASCULAR SCAFFOLDS, DILUTION:NA
He F,Xiao H,Cai Y,Zhang N.ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer.Cell Commun Signal.2021 May 12;19(1):53.doi:10.1186/s12964-021-00734-x.PMID:33980247;PMCID:PMC8117505.
Species: Human,Mouse
EK0539 usage in article: APP:ELISA, SAMPLE:HEK293T CELL, DILUTION:NA
Limnios IJ,Chau YQ,Skabo SJ,Surrao DC,O‘Neill HC.Efficient differentiation of human embryonic stem cells to retinal pigment epithelium under defined conditions.Stem Cell Res Ther.2021 Apr 21;12(1):248.doi:10.1186/s13287-021-02316-7.PMID:33883023;PMCID:PMC8058973.
Species: Human
EK0539 usage in article: APP:ELISA, SAMPLE:HESC-RPE CELL, DILUTION:NA
Mohan, N.,Luo, X.,Shen, Y.,Olson, Z.,Agrawal, A.,Endo, Y.,Rotstein,D. S.,Pelosof, L. C.,& Wu, W. J.(2021). A Novel Bispecific Antibody Targeting EGFR and VEGFR2 Is Effective against Triple Negative Breast Cancer via Multiple Mechanisms of Action. Cancers,13(5),1027.https://doi.org/10.3390/cancers13051027
Species: Human,Mouse
EK0539 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION:NA
Song Y,Wang M,Tong H,Tan Y,Hu X,Wang K,Wan X. Plasma exosomes from endometrial cancer patients contain LGALS3BP to promote endometrial cancer progression. Oncogene.2020 Nov 18.doi:10.1038/s41 388-020-01555-x.Epub ahead of print.PMID:33208911.
Species: Human,Mouse
EK0539 usage in article: APP:ELISA, SAMPLE:HUVECS AND SPEC2 CELLS, DILUTION:NA
Dai J,Cheng Y,Wu J,Wang Q,Wang W,Yang J,Zhao Z,Lou X,Xia F,Wang S,Tang BZ. Modular Peptide Probe for Pre/Intra/Postoperative Therapeutic to Reduce Recurrence in Ovarian Cancer. ACS Nano.2020 Nov 11.doi: 10.1021/acsnano.9b09818.Epub ahead of print.PMID:331
Species: Human,Mouse
EK0539 usage in article: APP:ELISA, SAMPLE:SKOV-3 AND PTX CELLS, DILUTION:NA
Expression of aquaporin-4 in human supratentorial meningiomas with peritumoral brain edema and correlation of VEGF with edema formation
IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma
The expressions of NGF and VEGF in the fracture tissues are closely associated with accelerated clavicle fracture healing in patients with traumatic brain injury
ERK Signaling Pathway Is Involved in HPV-16 E6 but not E7 Oncoprotein-Induced HIF-1α Protein Accumulation in NSCLC Cells
Molecular mechanism of inhibition of the abnormal proliferation of human umbilical vein endothelial cells by hydroxysafflor-yellow A
Poly(ε-caprolactone)/keratin/heparin/VEGF biocomposite mats for vascular tissue engineering
Re-Endothelialization Study on Endovascular Stents Seeded by Endothelial Cells through Up- or Downregulation of VEGF
Cytochalasin H Inhibits Angiogenesis via the Suppression of HIF-1α Protein Accumulation and VEGF Expression through PI3K/AKT/P70S6K and ERK1/2 Signaling Pathways in Non-Small Cell Lung Cancer Cells
Effect of small interference RNA targeting HIF-1alpha mediated by rAAV combined L: -ascorbate on pancreatic tumors in athymic mice
IL-6 mediates the signal pathway of JAK-STAT3-VEGF-C promoting growth, invasion and lymphangiogenesis in gastric cancer
Arsenic induces the expressions of angiogenesis-related factors through PI3K and MAPK pathways in SV-HUC-1 human uroepithelial cells
Epigallocatechin-3-gallate inhibits IGF-I-stimulated lung cancer angiogenesis through downregulation of HIF-1α and VEGF expression.
Case Control Study to Compare Serum Vascular Endothelial Growth Factor (VEGF) Level in Women with Recurrent Pregnancy Loss (RPL) Compared to Women with Term Pregnancy
Concentrations of VEGF and PlGF Decrease in Eyes After Intravitreal Conbercept Injection
The anti-cancer compound Nordy inhibits CXCR4-mediated production of IL-8 and VEGF by malignant human glioma cells
Antitumor activity of an adenovirus harboring two therapeutic genes, anti-VEGF ribozyme and human IL-24, in colon cancer.
Gene expression analysis of VEGF and its receptors and assessment of its serum level in unexplained recurrent spontaneous abortion.
Ge YL, Zhang X, Zhang JY, Hou L, Tian RH.The mechanisms on apoptosis by inhibiting VEGF expression in human breast cancer cells.
Histone demethylase KDM4D promotes gastrointestinal stromal tumor progression through HIF1?/VEGFA signalling
Cyclooxygenase-2 induces angiogenesis in pancreatic cancer mediated by prostaglandin E2
Growth Factor Variation in Two Types of Autologous Platelet Biomaterials: PRP Versus PRF
DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting ?-catenin Signaling to Induce Expression of VEGFA
High fibrosis indices in cerebrospinal fluid of patients with shunt-dependent post-traumatic chronic hydrocephalus
Elevated expression levels of serum insulin-like growth factor-1, tumor necrosis factor-? and vascular endothelial growth factor 165 might exacerbate type 2 diabetic nephropathy
Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene
Wogonin inhibits multiple myeloma-stimulated angiogenesis via c-Myc/VHL/HIF-1? signaling axis
Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with proliferative diabetic retinopathy
Radioactive 125I Seed Inhibits the Cell Growth, Migration, and Invasion of Nasopharyngeal Carcinoma by Triggering DNA Damage and Inactivating VEGF-A/ERK Signaling
Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs
Zou J, Wang N, Liu M, Bai Y, Wang H, Liu K, Zhang H, Xiao X, Wang K. J Cell Mol Med. 2018 May;22(5):2692-2705. doi: 10.1111/jcmm.13552. Epub 2018 Mar 7. Nucleolin mediated pro
A Y, Li Y, Zhao S. Oncol Lett. 2018 Feb;15(2):2534-2540. doi: 10.3892/ol.2017.7580. Epub 2017 Dec 11. The expression and underlying angiogenesis effect of DPC4 and VEGF on the progression of cervical carcinoma
Ma X, Li Z, Li T, Zhu L, Li Z, Tian N. Am J Transl Res. 2017 Nov 15;9(11):5012-5021. eCollection 2017. Long non-coding RNA HOTAIR enhances angiogenesis by induction of VEGFA expression in glioma cells and transmission to endothelial cells via glio...
Wang X, Cao P, Liu J, Du P, Wang Z, Chen W, Liu C, Wu Y. Med Sci Monit. 2017 Jan 4;23:46-56. 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts
Wu H, Deng J, Yu S, Wang X, Chen Y. J Huazhong Univ Sci Technolog Med Sci. 2010 Feb;30(1):108-12. Doi: 10.1007/S11596-010-0120-6. Epub 2010 Feb 14. The Inhibitory Effects Of Rh-Endostatin (Yh-16) In Combination With Radiotherapy On Lung Adenocarci...
Chuangui C, Peng T, Zhentao Y. Pathol Oncol Res. 2012 Oct;18(4):1021-7. Epub 2012 Apr 29. The Expression Of High Mobility Group Box 1 Is Associated With Lymph Node Metastasis And Poor Prognosis In Esophageal Squamous Cell Carcinoma.
Xu Yf, Ge Fj, Han B, Yang Xq, Su H, Zhao Ac, Zhao Mh, Yang Yb, Yang J. World J Gastroenterol. 2015 Mar 21;21(11):3256-65. Doi: 10.3748/Wjg.V21.I11.3256. High-Mobility Group Box 1 Expression And Lymph Node Metastasis In Intrahepatic Cholangiocarcin...
Li Jt, Wang Lf, Zhao Yl, Yang T, Li W, Zhao J, Yu F, Wang L, Meng Yl, Liu Nn, Zhu Xs, Gao Cf, Jia Lt, Yang Ag. Breast Cancer Res. 2014 Oct 14;16(5):454. Doi: 10.1186/S13058-014-0454-2. Nuclear Factor Of Activated T Cells 5 Maintained By Hotair Sup...
Bakir M, Bakir S, Sari I, Celik Vk, Gozel Mg, Engin A. J Med Virol. 2013 Oct;85(10):1794-801. Doi: 10.1002/Jmv.23666. Epub 2013 Jul 19. Evaluation Of The Relationship Between Serum Levels Of Vegf And Svegfr1 With Mortality And Prognosis In Patient...
Yang W, Zhang Y, Yu J, Li S. Eur J Surg Oncol. 2010 May;36(5):501-6. Doi: 10.1016/J.Ejso.2010.01.007. Epub 2010 Feb 23. The Low Expression Of Cd80 Correlated With The Vascular Endothelial Growth Factor In Esophageal Cancer Tissue.
Zhou L, Zhang K, Li J, Cui X, Wang A, Huang S, Zheng S, Lu Y, Chen W. Reprod Toxicol. 2013 Jun;37:62-9. Doi: 10.1016/J.Reprotox.2013.02.001. Epub 2013 Feb 11. Inhibition Of Vascular Endothelial Growth Factor-Mediated Angiogenesis Involved In Repro...
Chen J, Wei D, Zhuang H, Qiao Y, Tang B, Zhang X, Wei J, Fang S, Chen G, Du P, Huang X, Jiang W, Hu Q, Hua Zc. Mol Cell Proteomics. 2011 Jun;10(6):M111.009399. Doi: 10.1074/Mcp.M111.009399. Epub 2011 Apr 7. Proteomic Screening Of Anaerobically Reg...
Fu Z, Chen D, Cheng H, Wang F. Med Sci Monit. 2015 Jan 27;21:318-25. Doi: 10.12659/Msm.893265. Hypoxia-Inducible Factor-1?? Protects Cervical Carcinoma Cells From Apoptosis Induced By Radiation Via Modulation Of Vascular Endothelial Growth Factor ...
Liu Y, Lv L. J Huazhong Univ Sci Technolog Med Sci. 2004;24(5):470-2. Mechanism Of Elevated Vascular Endothelial Growth Factor Levels In Peritoneal Fluids From Patients With Endometriosis.
Li Xy, Lin Yc, Huang Wl, Hong Cq, Chen Jy, You Yj, Li Wb. Med Oncol. 2012 Jun;29(2):714-20. Doi: 10.1007/S12032-011-9904-1. Epub 2011 Mar 24. Zoledronic Acid Inhibits Proliferation And Impairs Migration And Invasion Through Downregulating Vegf And...
Ye X, Ren H, Zhang M, Sun Z, Jiang Ac, Xu G. Invest Ophthalmol Vis Sci. 2012 Jun 8;53(7):3481-9. Doi: 10.1167/Iovs.11-9076. Erk1/2 Signaling Pathway In The Release Of Vegf From M??ller Cells In Diabetes.
Shen J, Shen S, Das Un, Xu G. Lipids Health Dis. 2012 Jul 10;11:90. Doi: 10.1186/1476-511X-11-90. Effect Of Essential Fatty Acids On Glucose-Induced Cytotoxicity To Retinal Vascular Endothelial Cells.
Liu D, Cao G, Cen Y, Liu T, Peng W, Sun J, Li X, Zhou H. Int Immunopharmacol. 2013 Oct;17(2):237-44. Doi: 10.1016/J.Intimp.2013.06.002. Epub 2013 Jun 19. The Radiosensitizing Effect Of Cpg Odn107 On Human Glioma Cells Is Tightly Related To Its Ant...
Chai Zt, Kong J, Zhu Xd, Zhang Yy, Lu L, Zhou Jm, Wang Lr, Zhang Kz, Zhang Qb, Ao Jy, Wang M, Wu Wz, Wang L, Tang Zy, Sun Hc. Plos One. 2013 Oct 23;8(10):E77957. Doi: 10.1371/Journal.Pone.0077957. Ecollection 2013. Microrna-26A Inhibits Angiogenes...
Li Xq, Ouyang Zg, Zhang Sh, Liu H, Shang Y, Li Y, Zhen Ys. Cancer Biol Ther. 2014 Apr;15(4):398-408. Doi: 10.4161/Cbt.27626. Epub 2014 Jan 14. Synergistic Inhibition Of Angiogenesis And Glioma Cell-Induced Angiogenesis By The Combination Of Temozo...
Wang L, Zeng Y, Wang T, Liu H, Xiao H, Xiang H. Plos One. 2014 Jan 27;9(1):E86509. Doi: 10.1371/Journal.Pone.0086509. Ecollection 2014. Evaluating The Potential Bioactivity Of A Novel Compound Er1626.
Zhang H, Chen Y, Fan B, Wang W, Zhu W. Tumour Biol. 2015 May;36(5):3871-80. Doi: 10.1007/S13277-014-3029-Z. Epub 2015 Jan 11. Overexpression Of Vegf183 Promotes Murine Breast Cancer Cell Proliferation In Vitro And Induces Dilated Intratumoral Micr...
Das Kk, Jargar Jg, Saha S, Yendigeri Sm, Singh Sb. Indian J Pharmacol. 2015 May-Jun;47(3):285-91. Doi: 10.4103/0253-7613.157126. ??-Tocopherol Supplementation Prevents Lead Acetate And Hypoxia-Induced Hepatic Dysfunction.
Fan N, Zhang M, Xu K, Ke X, Ding Y, Wang D, Liu Y, Ning Y, Deng X, He H. Drug Alcohol Depend. 2015 Jul 1;152:57-61. Doi: 10.1016/J.Drugalcdep.2015.04.022. Epub 2015 May 8. Serum Level Of Vascular Endothelial Growth Factor Decreased In Chronic Keta...
Xu Wh, Ge Yl, Li Q, Zhang X, Duan Jh. World J Gastroenterol. 2007 Apr 14;13(14):2044-7. Inhibitory Effect Of Vascular Endothelial Growth Factors-Targeted Small Interfering Rna On Proliferation Of Gastric Cancer Cells.
En-Lin S, Sheng-Guo C, Hua-Qiao W. Gynecol Oncol. 2010 Jun;117(3):417-22. Doi: 10.1016/J.Ygyno.2009.12.016. Epub 2010 Apr 7. The Expression Of Efemp1 In Cervical Carcinoma And Its Relationship With Prognosis.
He W, Guo Z, Wen Y, Wang Q, Xie B, Zhu S, Wang Q. J Biomater Sci Polym Ed. 2012;23(1-4):315-31. Doi: 10.1163/092050610X550359. Epub 2011 Jan 18. Alginate-Graft-Pei As A Gene Delivery Vector With High Efficiency And Low Cytotoxicity.
Song El, Hou Yp, Yu Sp, Chen Sg, Huang Jt, Luo T, Kong Lp, Xu J, Wang Hq. Gynecol Oncol. 2011 Apr;121(1):174-80. Doi: 10.1016/J.Ygyno.2010.11.004. Epub 2010 Dec 15. Efemp1 Expression Promotes Angiogenesis And Accelerates The Growth Of Cervical Can...
Lu Zx, Liu Lt, Qi Xr. Int J Nanomedicine. 2011;6:1661-73. Doi: 10.2147/Ijn.S22293. Epub 2011 Aug 11. Development Of Small Interfering Rna Delivery System Using Pei-Peg-Aprpg Polymer For Antiangiogenic Vascular Endothelial Growth Factor Tumor-Targe...
Wang F, Liu S, Sun Q, Zhang L, Song Y, Sheng W, Xi S, Sun G. Occup Environ Med. 2014 Oct;71(10):675-80. Doi: 10.1136/Oemed-2014-102173. Epub 2014 Jul 4. Urinary Vegf And Pge2 Levels And The Association With Arsenical Metabolites In Copper-Smelting...
Zhang Hy, Fan Bl, Wu Xs, Mu Lm, Wang Wf, Zhu Wl. Mol Med Rep. 2015 May;11(5):4010. Doi: 10.3892/Mmr.2015.3178. Epub 2015 Jan 9. [Corrigendum] Overexpression Of The Chimeric Plasmin-Resistant Vegf165/Vegf183 (132-158) Protein In Murine Breast Cance...
Shi J, Liu F, Zhang W, Liu X, Lin B, Tang X. Oncol Rep. 2015 Jun;33(6):2972-80. Doi: 10.3892/Or.2015.3889. Epub 2015 Mar 31. Epigallocatechin-3-Gallate Inhibits Nicotine-Induced Migration And Invasion By The Suppression Of Angiogenesis And Epithel...
Sancakdar E, Guven As, Uysal Eb, Deveci K, G??lt??rk E. Pediatr Infect Dis J. 2015 Aug;34(8):E200-5. Doi: 10.1097/Inf.0000000000000706. Important Of Angiopoietic System In Evaluation Of Endothelial Damage In Children With Crimean-Congo Hemorrhagic...
Lin L, Han Mm, Wang F, Xu Ll, Yu Hx, Yang Py. Cell Death Dis. 2014 Oct 23;5:E1488. Doi: 10.1038/Cddis.2014.392. Cxcr7 Stimulates Mapk Signaling To Regulate Hepatocellular Carcinoma Progression.
Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.
Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ...
Zhang E, Feng X, Liu F, Zhang P, Liang J, Tang X. Plos One. 2014 Jul 24;9(7):E103440. Doi: 10.1371/Journal.Pone.0103440. Ecollection 2014. Roles Of Pi3K/Akt And C-Jun Signaling Pathways In Human Papillomavirus Type 16 Oncoprotein-Induced Hif-1??, ...
Chen L, Xiao Z, Meng Y, Zhao Y, Han J, Su G, Chen B, Dai J. Biomaterials. 2012 Feb;33(5):1437-44. Doi: 10.1016/J.Biomaterials.2011.10.056. Epub 2011 Nov 10. The Enhancement Of Cancer Stem Cell Properties Of Mcf-7 Cells In 3D Collagen Scaffolds For...
Liu Lj, Zhang J, Xiao Zf, Dai B, Sun My, Chen L, Chen B. J Biomed Mater Res B Appl Biomater. 2014 May;102(4):772-80. Doi: 10.1002/Jbm.B.33058. Epub 2013 Oct 21. Three-Dimensional Collagen Scaffold Enhances The Human Adenoid Cystic Carcinoma Cancer...
Chen J, Xu J, Li J, Du L, Chen T, Liu P, Peng S, Wang M, Song H. Int Immunopharmacol. 2015 May;26(1):147-52. Doi: 10.1016/J.Intimp.2015.03.025. Epub 2015 Mar 31. Epigallocatechin-3-Gallate Attenuates Lipopolysaccharide-Induced Mastitis In Rats Via...
Yang Py, Rui Yc, Lu L, Li Tj, Liu Sq, Yan Hx, Wang Hy. Life Sci. 2005 Sep 30;77(20):2529-39. Time Courses Of Vascular Endothelial Growth Factor And Intercellular Adhesion Molecule-1 Expressions In Aortas Of Atherosclerotic Rats.
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Customer Q&As
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22 Customer Q&As for Human VEGF ELISA Kit PicoKine®
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of VEGFA in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-10-16
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the VEGFA ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-10-16
Question
Q: Can VEGFA ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-08-25
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-08-25
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Z. Jackson
Verified customer
Asked: 2020-07-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-22
Question
I would like to know the epitopes and cross reactivity of this Human VEGF ELISA Kit PicoKine antibody as well as the animal of origin.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
The Human VEGF ELISA Kit PicoKine, Capture antibody; monoclonal mouse and the immunogen sequence is Ala27
Boster Scientific Support
Answered: 2020-03-23
Question
Q: how to analyze ELISA data? I measured VEGFA level in plasma.
Verified Customer
Verified customer
Asked: 2020-02-09
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-09
Question
I want to measure VEGF in urine using ELISA. I saw your product Human VEGF ELISA Kit PicoKine ,is it suitable for urine samples?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
The Human VEGF ELISA Kit PicoKine is suitable in detecting VEGF in biological fluids such as cell culture supernatant, serum and plasma.
Boster Scientific Support
Answered: 2020-01-16
Question
Has your lab ever tested EK0539 in urine samples? Or, have you had a customer that has had success/failure in testing this kit with urine samples?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Our lab technicians haven't
Boster Scientific Support
Answered: 2019-11-25
Question
What is being used in your method when transfering genes to sf21 cells for EK0539?
Verified customer
Asked: 2019-11-14
Answer
For the Human VEGF ELISA Kit PicoKine™ (EK0539), we use baculovirus to transfer genes to sf21 cells.
Boster Scientific Support
Answered: 2019-11-14
Question
How come I saw no signal for running your kit with my VEGF plasma sample?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
First of all, the amount of VEGF antigen in plasma is much lower than that in serum, and it is possible that the antigen is undetectable by the assay. You may use serum instead of plasma if applicable.
Boster Scientific Support
Answered: 2019-11-13
Question
What is a possible cause for obtaining low data results with Human VEGF ELISA Kit PicoKine?
Verified customer
Asked: 2019-11-11
Answer
The lower data might be caused by some factors which include but not limited to unstable temperature during transportation and variation of operation conditions.
Boster Scientific Support
Answered: 2019-11-18
Question
Have other isoforms been tested for cross-reactivity with EK0539?
Verified customer
Asked: 2019-09-25
Answer
The Human VEGF ELISA Kit PicoKine EK0539 is cross-reactive with their specific targets only.
Boster Scientific Support
Answered: 2019-09-26
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Dayal
Verified customer
Asked: 2019-08-10
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-10
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification VEGFA using the EK0539 Human VEGFA Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-30
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-09-30
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-09-29
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-09-29
Question
Q: how to thaw whole blood sample for VEGFA ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFA for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-09-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-06-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-26
Question
Q: if the enzyme conjugated VEGFA antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the VEGFA antigen?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-03-23
Question
Does this kit detect all forms of VEGF or just VEGFA
Verified Customer
Verified customer
Asked: 2017-08-02
Answer
This assay detects VEGFA
Boster Scientific Support
Answered: 2017-08-02
Question
Are the mouse, rat, and human VEGF kits truly specific for each species or do they cross react? Which isoforms of VEGF do the human, mouse and rat kit detect? Keyword: specificity
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
Our VEGF Elisa kits are truly specific for each species, and they detect 165 amino acid form.
Boster Scientific Support
Answered: 2017-06-08
Question
I would like to purchase Human VEGF ELISA Kit PicoKine and use it on protein extracts in lysis buffer containing 8M urea. Do you have any information if the ELISA work with Urea 1M after dilution of the sample in diluent buffer?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
Unfortunately, we have not tested the effects of Urea containing lysis buffer on samples with this antibody Human VEGF ELISA Kit PicoKine, so we do not have any idea how well this would work. But it is important to note that Urea is a Chaotropic agent at 6 - 8 M that means it can denature proteins, thus this buffer formula may unfavorably affect the VEGF protein causing it not to be recognized by the ELISA.
Boster Scientific Support
Answered: 2017-05-12
Question
Can you tell me how to troubleshoot?
Verified Customer
Verified customer
Asked: 2016-09-15
Answer
To troubleshoot, you should try to repeat your experiment without diluting your plasma samples. If this approach still does not work, try to increase your sample incubation time to 2.5 hours (at room temperature). To minimize any potential sample losses, we recommend you pilot your experiment using standards and a small number of samples.
Boster Scientific Support
Answered: 2016-09-15
Question
For the kit EK0539 a customer ask if the kit detects all forms of VEGF or only VEGFA?
Verified Customer
Verified customer
Asked: 2014-02-10
Answer
EK0539 detects just VEGFA.
Boster Scientific Support
Answered: 2014-02-10
