Human VEGF ELISA Kit PicoKine®

VEGFA ELISA kit for Human

Human VEGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. Cited in 179 publication(s).

Product Info Summary

SKU: EK0539
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human VEGF ELISA Kit PicoKine®

View all VEGFA ELISA kits

SKU/Catalog Number

EK0539

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human VEGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human VEGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0539)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-R191

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0539 is reactive to VEGFA in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0539 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of VEGFA

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.

Kit Components

Catalog Number Description Quantity
EK0539-CAP Anti-Human VEGFA Pre-coated 96-well strip microplate 1
EK0539-ST Human VEGFA Standard 2 vials, 10 ng/tube
EK0539-DA Human VEGFA Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0200.0550.1130.1890.3450.5891.1061.913

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect VEGFA, Dilution ratio of 1:1, concentration in serum is around 80pg/ml.

Some articles we found to cite concentrations of VEGFA in samples: 25374209 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human VEGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7227094676254955
Standard deviation4.5321.3359.599.6224.3874.49
CV (%)9.6%7.9%9.6%7.8%9.6%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of VEGFA in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 172808271764.816.3%
Sample 227026725224525810.354%
Sample 39468971026103797657.795.9%
*number of samples for each test n=16.

Gene/Protein Information For VEGFA (Source: Uniprot.Org, NCBI)

Uniprot ID

P15692

Gene ID

7422

Gene Name

VEGFA

Full Name

Vascular endothelial growth factor A

Weight

27.042kDa

Superfamily

PDGF/VEGF growth factor family

Alternative Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VEGFA, check out the VEGFA Infographic

VEGFA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VEGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0539 has been cited in 179 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Long non-coding RNA HOTAIR enhances angiogenesis by induction of VEGFA expression in glioma cells and transmission to endothelial cells via glioma cell derived-extracellular vesicles

Cytochalasin H Inhibits Angiogenesis via the Suppression of HIF-1α Protein Accumulation and VEGF Expression through PI3K/AKT/P70S6K and ERK1/2 Signaling Pathways in Non-Small Cell Lung Cancer Cells

The role of monoamine oxidase A in HPV-16 E7-induced epithelial-mesenchymal transition and HIF-1α protein accumulation in non-small cell lung cancer cells

Serum Vascular Endothelial Growth Factor and Vascular Endothelial Growth Factor Receptor-1 Levels in Patients With Fibromyalgia Syndrome

Relationship between single nucleotide polymorphisms in the 3’-untranslated region of the vascular endothelial growth factor gene and susceptibility to diabetic peripheral neuropathy in China

Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene

Clinical significance of survivin and vascular endothelial growth factor mRNA detection in the peripheral whole blood of breast cancer patients.

Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with proliferative diabetic retinopathy

Fucoidan inhibits angiogenesis induced by multiple myeloma cells

Cyclooxygenase‑2 induces angiogenesis in pancreatic cancer mediated by prostaglandin E2

Antitumor activity of an adenovirus harboring two therapeutic genes, anti-VEGF ribozyme and human IL-24, in colon cancer

Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010

FOXP3 facilitates the invasion and metastasis of non‑small cell lung cancer cells through regulating VEGF, EMT and the Notch1/Hes1 pathway

Inhibitory effect of vascular endothelial growth factors-targeted small interfering RNA on proliferation of gastric cancer cells

ERK Signaling Pathway Is Involved in HPV-16 E6 but not E7 Oncoprotein-Induced HIF-1α Protein Accumulation in NSCLC Cells

Mesenchymal Stem Cells and Islet Cotransplantation in Diabetic Rats: Improved Islet Graft Revascularization and Function by Human Adipose Tissue-Derived Stem Cells Preconditioned with Natural Molecules:

Plasma Lipid Profiling Contributes to Untangle the Complexity of Moyamoya Arteriopathy

A Novel Bispecific Antibody Targeting EGFR and VEGFR2 Is Effective against Triple Negative Breast Cancer via Multiple Mechanisms of Action

The P2X7 Receptor 489C>T Gain of Function Polymorphism Favors HHV-6A Infection and Associates With Female Idiopathic Infertility

Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin

Predictors for the efficacy of Endostar combined with neoadjuvant chemotherapy for stage IIIA (N2) NSCLC

Investigation of the maternal and cord plasma levels of IL-1 beta, TNF-alpha and VEGF in early membrane rupture

Molecular mechanism of inhibition of the abnormal proliferation of human umbilical vein endothelial cells by hydroxysafflor-yellow A

β-Tryptase up-regulates vascular endothelial growth factor expression via proteinase-activated receptor-2 and mitogen-activated protein kinase pathways in bone marrow stromal cells in acute myeloid leukemia

Distinct peritoneal fluid ghrelin and leptin in infertile women with endometriosis and their correlation with interleukin-6 and vascular endothelial growth factor

NDRG2 inhibition facilitates angiogenesis of hepatocellular carcinoma

Radioactive 125I Seed Inhibits the Cell Growth, Migration, and Invasion of Nasopharyngeal Carcinoma by Triggering DNA Damage and Inactivating VEGF-A/ERK Signaling

Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs

Plumbagin Restrains Hepatocellular Carcinoma Angiogenesis by Stromal Cell-Derived Factor (SDF-1)/CXCR4-CXCR7 Axis

VEGF Gene Polymorphisms Affect Serum Protein Levels and Alter Disease Activity and Synovial Lesions in Rheumatoid Arthritis

PREFERENTIAL EXPRESSION OF CHEMOKINE RECEPTOR CXCR4 BY HIGHLY MALIGNANT HUMAN GLIOMAS AND ITS ASSOCIATION WITH POOR PATIENT SURVIVAL

Efficient differentiation of human embryonic stem cells to retinal pigment epithelium under defined conditions

The crude ethanol extract of Periplaneta americana L. stimulates wound healing in vitro & in vivo

Efficacy of wIRA in the treatment of sacroiliitis in male patients with ankylosing spondylitis and its effect on serum VEGF levels

Novel HDGF/HIF-1α/VEGF axis in oral cancer impacts disease prognosis

The Effect of Autophagic Activity on the Function of Apheresis Platelets and on the Efficacy of Clinical Platelet Transfusion

Low Oxygen Consumption is Related to a Hypomethylation and an Increased Secretion of IL-6 in Obese Subjects with Sleep Apnea-Hypopnea Syndrome

Epigallocatechin-3-Gallate Inhibits IGF-I-Stimulated Lung Cancer Angiogenesis through Downregulation of HIF-1α and VEGF Expression

Amarogentin Inhibits Liver Cancer Cell Angiogenesis after Insufficient Radiofrequency Ablation via Affecting Stemness and the p53-Dependent VEGFA/Dll4/Notch1 Pathway

Expression of B2 Receptor on Circulating CD34-Positive Cells and Outcomes of Myocardial Infarction

Long Noncoding RNA LINC00657 Acting as a miR-590-3p Sponge to Facilitate Low Concentration Oxidized Low-Density Lipoprotein–Induced Angiogenesis

Polyethylene Glycol-Modified Pigment Epithelial-Derived Factor: New Prospects for Treatment of Retinal Neovascularization

Assessment of cervicovaginal vascular endothelial growth factor in predicting preterm delivery

Arctiin attenuates high glucose‐induced human retinal capillary endothelial cell proliferation by regulating ROCK1/PTEN/PI3K/Akt/VEGF pathway in vitro

Immunogenicity moderation effect of interleukin-24 on myelogenous leukemia cells

Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at −20 °C without any preservation agent

Cytokine profile of breast cell lines after different radiation doses

Expression of soluble Flt‐1 gene in Pichia pastoris and the effect of the product on multiple‐myeloma cells in vitro

PEGylated HM-3 presents anti-rheumatic bioactivity by inhibiting angiogenesis and inflammation

Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction

LncRNA ZNFTR functions as an inhibitor in pancreatic cancer by modulating ATF3/ZNF24/VEGFA pathway

Inhibition of SIRT2 limits tumour angiogenesis via inactivation of the STAT3/VEGFA signalling pathway

Bevacizumab or fibronectin gene editing inhibits the osteoclastogenic effects of fibroblasts derived from human radicular cysts

Plasma exosomes from endometrial cancer patients contain LGALS3BP to promote endometrial cancer progression

Extracellular signal-regulated kinase 5 increases radioresistance of lung cancer cells by enhancing the DNA damage response

Microvesicles secreted from human multiple myeloma cells promote angiogenesis

Sulfonate Groups and Saccharides as Essential Structural Elements in Heparin-Mimicking Polymers Used as Surface Modifiers: Optimization of Relative Contents for Antithrombogenic Properties

In Situ Prevascularization Strategy with Three-Dimensional Porous Conduits for Neural Tissue Engineering

Cascade Signal Amplification Strategy for Subattomolar Protein Detection by Rolling Circle Amplification and Quantum Dots Tagging

Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment

SIX4 activates Akt and promotes tumor angiogenesis

Allometric Scaling of Therapeutic Monoclonal Antibodies Using Antigen Concentration as a Correction Factor: Application to the Human Clearance Prediction

Quantification of platelets and platelet derived growth factors from platelet-rich-plasma (PRP) prepared at different centrifugal force (g) and time

Arsenic induces the expressions of angiogenesis-related factors through PI3K and MAPK pathways in SV-HUC-1 human uroepithelial cells

Platforms to test the Temporospatial capabilities of carrier Systems in Delivering Growth Factors to benefit vascular bioengineering

The role of circulating soluble fms-like tyrosine kinase-1 in patients with diabetic foot ulcer: A possible mechanism of pathogenesis via a novel link between oxidative stress, inflammation and angiogenesis

Effect of blood purification on serum miR-126 and VEGF levels in the process of atherosclerosis in uremic patients under maintenance hemodialysis

Reported Prestroke Physical Activity Is Associated with Vascular Endothelial Growth Factor Expression and Good Outcomes after Stroke

Expression of TGF-beta1 in Smooth Muscle Cells Regulates Endothelial Progenitor Cells Migration and Differentiation1

VEGF165b mutant with a prolonged half-life and enhanced anti-tumor potency in a mouse model

Vascular endothelial growth factor gene polymorphisms in patients with rosacea: A case-control study

The mechanisms on apoptosis by inhibiting VEGF expression in human breast cancer cells

Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation

Biotransfomation of cyperenoic acid by Cunninghamella elegans AS 3.2028 and the potent anti-angiogenic activities of its metabolites

In vitro and in vivo study of hydralazine, a potential anti-angiogenic agent

DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting β-catenin Signaling to Induce Expression of VEGFA

Proliferation, behavior, and differentiation of osteoblasts on surfaces of different microroughness

Effect of different enamel matrix derivative proteins on behavior and differentiation of endothelial cells

Synthesis of a chitosan-based photo-sensitive hydrogel and its biocompatibility and biodegradability

Hypoxia-induced ebv-circLMP2A promotes angiogenesis in EBV-associated gastric carcinoma through the KHSRP/VHL/HIF1α/VEGFA pathway

Endostar, a modified recombinant human endostatin, exhibits synergistic effects with dexamethasone on angiogenesis and hepatoma growth

Periplaneta Americana L. as a novel therapeutics accelerates wound repair and regeneration

Downregulating FPR restrains xenograft tumors by impairing the angiogenic potential and invasive capability of malignant glioma cells

Expression of miR-217 and HIF-1α/VEGF pathway in patients with diabetic foot ulcer and its effect on angiogenesis of diabetic foot ulcer rats

Concentrations of VEGF and PlGF Decrease in Eyes After Intravitreal Conbercept Injection

Association of High Serum Levels of Growth Factors with Good Outcome in Ischemic Stroke: a Multicenter Study

Growth Factor Variation in Two Types of Autologous Platelet Biomaterials: PRP Versus PRF

Down-regulation of HPV18 E6, E7, or VEGF expression attenuates malignant biological behavior of human cervical cancer cells

Pulsed Electromagnetic Fields Modulate miRNAs During Osteogenic Differentiation of Bone Mesenchymal Stem Cells: a Possible Role in the Osteogenic-angiogenic Coupling

Serum vascular endothelial growth factor and angiogenesis are related to the prognosis of patients with gastrointestinal stromal tumors

Vascular Endothelial Growth Factor Enhanced the Angiogenesis Response of Human Umbilical Cord-Derived Mesenchymal Stromal Cells in a Rat Model of Radiation Myelopathy

The anti-cancer compound Nordy inhibits CXCR4-mediated production of IL-8 and VEGF by malignant human glioma cells

Adipose tissue extract shows potential for wound healing: in vitro proliferation and migration of cell types contributing to wound healing in the presence of adipose tissue preparation and platelet rich plasma

Impact of ER520, a candidate of selective estrogen receptor modulators, on in vitro cell growth, migration, invasion, angiogenesis and in vivo tumor xenograft of human breast cancer cells

Fascaplysin, a selective CDK4 inhibitor, exhibit anti-angiogenic activity in vitro and in vivo

Uremic Serum Impairs Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stromal Cells

Exosomes derived from adipose tissue, bone marrow, and umbilical cord blood for cardioprotection after myocardial infarction

Heparin/poly‐l‐lysine nanoplatform with growth factor delivery for surface modification of cardiovascular stents: The influence of vascular endothelial growth factor loading

Poly(ε‐caprolactone)/keratin/heparin/VEGF biocomposite mats for vascular tissue engineering

A simple electrochemical aptasensor for ultrasensitive protein detection using cyclic target-induced primer extension
Species: Human

Inducing a Transient Increase in Blood–Brain Barrier Permeability for Improved Liposomal Drug Therapy of Glioblastoma Multiforme
Species: Human

Modular Peptide Probe for Pre/Intra/Postoperative Therapeutic to Reduce Recurrence in Ovarian Cancer
Species: Mouse

Argueta LB,Niles JA,Sakamoto J,Liu X,Vega SP,Frank L,Paessler M,Cortiella J,Nichols JE.Platforms to test the Temporospatial capabilities of carrier Systems in Delivering Growth Factors to benefit vascular bioengineering.Nanomedicine.2021 Jun 17:102419.doi:10.1016/j.nano.2021.102419.Epub ahead of print.PMID:34147665.
Species: Porcine
EK0539 usage in article: APP:ELISA, SAMPLE:VASCULAR SCAFFOLDS, DILUTION:NA

He F,Xiao H,Cai Y,Zhang N.ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer.Cell Commun Signal.2021 May 12;19(1):53.doi:10.1186/s12964-021-00734-x.PMID:33980247;PMCID:PMC8117505.
Species: Human,Mouse
EK0539 usage in article: APP:ELISA, SAMPLE:HEK293T CELL, DILUTION:NA

Limnios IJ,Chau YQ,Skabo SJ,Surrao DC,O‘Neill HC.Efficient differentiation of human embryonic stem cells to retinal pigment epithelium under defined conditions.Stem Cell Res Ther.2021 Apr 21;12(1):248.doi:10.1186/s13287-021-02316-7.PMID:33883023;PMCID:PMC8058973.
Species: Human
EK0539 usage in article: APP:ELISA, SAMPLE:HESC-RPE CELL, DILUTION:NA

Mohan, N.,Luo, X.,Shen, Y.,Olson, Z.,Agrawal, A.,Endo, Y.,Rotstein,D. S.,Pelosof, L. C.,& Wu, W. J.(2021). A Novel Bispecific Antibody Targeting EGFR and VEGFR2 Is Effective against Triple Negative Breast Cancer via Multiple Mechanisms of Action. Cancers,13(5),1027.https://doi.org/10.3390/cancers13051027
Species: Human,Mouse
EK0539 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION:NA

Song Y,Wang M,Tong H,Tan Y,Hu X,Wang K,Wan X. Plasma exosomes from endometrial cancer patients contain LGALS3BP to promote endometrial cancer progression. Oncogene.2020 Nov 18.doi:10.1038/s41 388-020-01555-x.Epub ahead of print.PMID:33208911.
Species: Human,Mouse
EK0539 usage in article: APP:ELISA, SAMPLE:HUVECS AND SPEC2 CELLS, DILUTION:NA

Dai J,Cheng Y,Wu J,Wang Q,Wang W,Yang J,Zhao Z,Lou X,Xia F,Wang S,Tang BZ. Modular Peptide Probe for Pre/Intra/Postoperative Therapeutic to Reduce Recurrence in Ovarian Cancer. ACS Nano.2020 Nov 11.doi: 10.1021/acsnano.9b09818.Epub ahead of print.PMID:331
Species: Human,Mouse
EK0539 usage in article: APP:ELISA, SAMPLE:SKOV-3 AND PTX CELLS, DILUTION:NA

Expression of aquaporin-4 in human supratentorial meningiomas with peritumoral brain edema and correlation of VEGF with edema formation

IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma

The expressions of NGF and VEGF in the fracture tissues are closely associated with accelerated clavicle fracture healing in patients with traumatic brain injury

ERK Signaling Pathway Is Involved in HPV-16 E6 but not E7 Oncoprotein-Induced HIF-1α Protein Accumulation in NSCLC Cells

Molecular mechanism of inhibition of the abnormal proliferation of human umbilical vein endothelial cells by hydroxysafflor-yellow A

Poly(ε-caprolactone)/keratin/heparin/VEGF biocomposite mats for vascular tissue engineering

Re-Endothelialization Study on Endovascular Stents Seeded by Endothelial Cells through Up- or Downregulation of VEGF

Cytochalasin H Inhibits Angiogenesis via the Suppression of HIF-1α Protein Accumulation and VEGF Expression through PI3K/AKT/P70S6K and ERK1/2 Signaling Pathways in Non-Small Cell Lung Cancer Cells

Effect of small interference RNA targeting HIF-1alpha mediated by rAAV combined L: -ascorbate on pancreatic tumors in athymic mice

IL-6 mediates the signal pathway of JAK-STAT3-VEGF-C promoting growth, invasion and lymphangiogenesis in gastric cancer

Arsenic induces the expressions of angiogenesis-related factors through PI3K and MAPK pathways in SV-HUC-1 human uroepithelial cells

Epigallocatechin-3-gallate inhibits IGF-I-stimulated lung cancer angiogenesis through downregulation of HIF-1α and VEGF expression.

Case Control Study to Compare Serum Vascular Endothelial Growth Factor (VEGF) Level in Women with Recurrent Pregnancy Loss (RPL) Compared to Women with Term Pregnancy

Concentrations of VEGF and PlGF Decrease in Eyes After Intravitreal Conbercept Injection

The anti-cancer compound Nordy inhibits CXCR4-mediated production of IL-8 and VEGF by malignant human glioma cells

Antitumor activity of an adenovirus harboring two therapeutic genes, anti-VEGF ribozyme and human IL-24, in colon cancer.

Gene expression analysis of VEGF and its receptors and assessment of its serum level in unexplained recurrent spontaneous abortion.

Ge YL, Zhang X, Zhang JY, Hou L, Tian RH.The mechanisms on apoptosis by inhibiting VEGF expression in human breast cancer cells.

Histone demethylase KDM4D promotes gastrointestinal stromal tumor progression through HIF1?/VEGFA signalling

Cyclooxygenase-2 induces angiogenesis in pancreatic cancer mediated by prostaglandin E2

Growth Factor Variation in Two Types of Autologous Platelet Biomaterials: PRP Versus PRF

DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting ?-catenin Signaling to Induce Expression of VEGFA

High fibrosis indices in cerebrospinal fluid of patients with shunt-dependent post-traumatic chronic hydrocephalus

Elevated expression levels of serum insulin-like growth factor-1, tumor necrosis factor-? and vascular endothelial growth factor 165 might exacerbate type 2 diabetic nephropathy

Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene

Wogonin inhibits multiple myeloma-stimulated angiogenesis via c-Myc/VHL/HIF-1? signaling axis

Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with proliferative diabetic retinopathy

Radioactive 125I Seed Inhibits the Cell Growth, Migration, and Invasion of Nasopharyngeal Carcinoma by Triggering DNA Damage and Inactivating VEGF-A/ERK Signaling

Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs

Zou J, Wang N, Liu M, Bai Y, Wang H, Liu K, Zhang H, Xiao X, Wang K. J Cell Mol Med. 2018 May;22(5):2692-2705. doi: 10.1111/jcmm.13552. Epub 2018 Mar 7. Nucleolin mediated pro

A Y, Li Y, Zhao S. Oncol Lett. 2018 Feb;15(2):2534-2540. doi: 10.3892/ol.2017.7580. Epub 2017 Dec 11. The expression and underlying angiogenesis effect of DPC4 and VEGF on the progression of cervical carcinoma

Ma X, Li Z, Li T, Zhu L, Li Z, Tian N. Am J Transl Res. 2017 Nov 15;9(11):5012-5021. eCollection 2017. Long non-coding RNA HOTAIR enhances angiogenesis by induction of VEGFA expression in glioma cells and transmission to endothelial cells via glio...

Wang X, Cao P, Liu J, Du P, Wang Z, Chen W, Liu C, Wu Y. Med Sci Monit. 2017 Jan 4;23:46-56. 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts

Wu H, Deng J, Yu S, Wang X, Chen Y. J Huazhong Univ Sci Technolog Med Sci. 2010 Feb;30(1):108-12. Doi: 10.1007/S11596-010-0120-6. Epub 2010 Feb 14. The Inhibitory Effects Of Rh-Endostatin (Yh-16) In Combination With Radiotherapy On Lung Adenocarci...

Chuangui C, Peng T, Zhentao Y. Pathol Oncol Res. 2012 Oct;18(4):1021-7. Epub 2012 Apr 29. The Expression Of High Mobility Group Box 1 Is Associated With Lymph Node Metastasis And Poor Prognosis In Esophageal Squamous Cell Carcinoma.

Xu Yf, Ge Fj, Han B, Yang Xq, Su H, Zhao Ac, Zhao Mh, Yang Yb, Yang J. World J Gastroenterol. 2015 Mar 21;21(11):3256-65. Doi: 10.3748/Wjg.V21.I11.3256. High-Mobility Group Box 1 Expression And Lymph Node Metastasis In Intrahepatic Cholangiocarcin...

Li Jt, Wang Lf, Zhao Yl, Yang T, Li W, Zhao J, Yu F, Wang L, Meng Yl, Liu Nn, Zhu Xs, Gao Cf, Jia Lt, Yang Ag. Breast Cancer Res. 2014 Oct 14;16(5):454. Doi: 10.1186/S13058-014-0454-2. Nuclear Factor Of Activated T Cells 5 Maintained By Hotair Sup...

Bakir M, Bakir S, Sari I, Celik Vk, Gozel Mg, Engin A. J Med Virol. 2013 Oct;85(10):1794-801. Doi: 10.1002/Jmv.23666. Epub 2013 Jul 19. Evaluation Of The Relationship Between Serum Levels Of Vegf And Svegfr1 With Mortality And Prognosis In Patient...

Yang W, Zhang Y, Yu J, Li S. Eur J Surg Oncol. 2010 May;36(5):501-6. Doi: 10.1016/J.Ejso.2010.01.007. Epub 2010 Feb 23. The Low Expression Of Cd80 Correlated With The Vascular Endothelial Growth Factor In Esophageal Cancer Tissue.

Zhou L, Zhang K, Li J, Cui X, Wang A, Huang S, Zheng S, Lu Y, Chen W. Reprod Toxicol. 2013 Jun;37:62-9. Doi: 10.1016/J.Reprotox.2013.02.001. Epub 2013 Feb 11. Inhibition Of Vascular Endothelial Growth Factor-Mediated Angiogenesis Involved In Repro...

Chen J, Wei D, Zhuang H, Qiao Y, Tang B, Zhang X, Wei J, Fang S, Chen G, Du P, Huang X, Jiang W, Hu Q, Hua Zc. Mol Cell Proteomics. 2011 Jun;10(6):M111.009399. Doi: 10.1074/Mcp.M111.009399. Epub 2011 Apr 7. Proteomic Screening Of Anaerobically Reg...

Fu Z, Chen D, Cheng H, Wang F. Med Sci Monit. 2015 Jan 27;21:318-25. Doi: 10.12659/Msm.893265. Hypoxia-Inducible Factor-1?? Protects Cervical Carcinoma Cells From Apoptosis Induced By Radiation Via Modulation Of Vascular Endothelial Growth Factor ...

Liu Y, Lv L. J Huazhong Univ Sci Technolog Med Sci. 2004;24(5):470-2. Mechanism Of Elevated Vascular Endothelial Growth Factor Levels In Peritoneal Fluids From Patients With Endometriosis.

Li Xy, Lin Yc, Huang Wl, Hong Cq, Chen Jy, You Yj, Li Wb. Med Oncol. 2012 Jun;29(2):714-20. Doi: 10.1007/S12032-011-9904-1. Epub 2011 Mar 24. Zoledronic Acid Inhibits Proliferation And Impairs Migration And Invasion Through Downregulating Vegf And...

Ye X, Ren H, Zhang M, Sun Z, Jiang Ac, Xu G. Invest Ophthalmol Vis Sci. 2012 Jun 8;53(7):3481-9. Doi: 10.1167/Iovs.11-9076. Erk1/2 Signaling Pathway In The Release Of Vegf From M??ller Cells In Diabetes.

Shen J, Shen S, Das Un, Xu G. Lipids Health Dis. 2012 Jul 10;11:90. Doi: 10.1186/1476-511X-11-90. Effect Of Essential Fatty Acids On Glucose-Induced Cytotoxicity To Retinal Vascular Endothelial Cells.

Liu D, Cao G, Cen Y, Liu T, Peng W, Sun J, Li X, Zhou H. Int Immunopharmacol. 2013 Oct;17(2):237-44. Doi: 10.1016/J.Intimp.2013.06.002. Epub 2013 Jun 19. The Radiosensitizing Effect Of Cpg Odn107 On Human Glioma Cells Is Tightly Related To Its Ant...

Chai Zt, Kong J, Zhu Xd, Zhang Yy, Lu L, Zhou Jm, Wang Lr, Zhang Kz, Zhang Qb, Ao Jy, Wang M, Wu Wz, Wang L, Tang Zy, Sun Hc. Plos One. 2013 Oct 23;8(10):E77957. Doi: 10.1371/Journal.Pone.0077957. Ecollection 2013. Microrna-26A Inhibits Angiogenes...

Li Xq, Ouyang Zg, Zhang Sh, Liu H, Shang Y, Li Y, Zhen Ys. Cancer Biol Ther. 2014 Apr;15(4):398-408. Doi: 10.4161/Cbt.27626. Epub 2014 Jan 14. Synergistic Inhibition Of Angiogenesis And Glioma Cell-Induced Angiogenesis By The Combination Of Temozo...

Wang L, Zeng Y, Wang T, Liu H, Xiao H, Xiang H. Plos One. 2014 Jan 27;9(1):E86509. Doi: 10.1371/Journal.Pone.0086509. Ecollection 2014. Evaluating The Potential Bioactivity Of A Novel Compound Er1626.

Zhang H, Chen Y, Fan B, Wang W, Zhu W. Tumour Biol. 2015 May;36(5):3871-80. Doi: 10.1007/S13277-014-3029-Z. Epub 2015 Jan 11. Overexpression Of Vegf183 Promotes Murine Breast Cancer Cell Proliferation In Vitro And Induces Dilated Intratumoral Micr...

Das Kk, Jargar Jg, Saha S, Yendigeri Sm, Singh Sb. Indian J Pharmacol. 2015 May-Jun;47(3):285-91. Doi: 10.4103/0253-7613.157126. ??-Tocopherol Supplementation Prevents Lead Acetate And Hypoxia-Induced Hepatic Dysfunction.

Fan N, Zhang M, Xu K, Ke X, Ding Y, Wang D, Liu Y, Ning Y, Deng X, He H. Drug Alcohol Depend. 2015 Jul 1;152:57-61. Doi: 10.1016/J.Drugalcdep.2015.04.022. Epub 2015 May 8. Serum Level Of Vascular Endothelial Growth Factor Decreased In Chronic Keta...

Xu Wh, Ge Yl, Li Q, Zhang X, Duan Jh. World J Gastroenterol. 2007 Apr 14;13(14):2044-7. Inhibitory Effect Of Vascular Endothelial Growth Factors-Targeted Small Interfering Rna On Proliferation Of Gastric Cancer Cells.

En-Lin S, Sheng-Guo C, Hua-Qiao W. Gynecol Oncol. 2010 Jun;117(3):417-22. Doi: 10.1016/J.Ygyno.2009.12.016. Epub 2010 Apr 7. The Expression Of Efemp1 In Cervical Carcinoma And Its Relationship With Prognosis.

He W, Guo Z, Wen Y, Wang Q, Xie B, Zhu S, Wang Q. J Biomater Sci Polym Ed. 2012;23(1-4):315-31. Doi: 10.1163/092050610X550359. Epub 2011 Jan 18. Alginate-Graft-Pei As A Gene Delivery Vector With High Efficiency And Low Cytotoxicity.

Song El, Hou Yp, Yu Sp, Chen Sg, Huang Jt, Luo T, Kong Lp, Xu J, Wang Hq. Gynecol Oncol. 2011 Apr;121(1):174-80. Doi: 10.1016/J.Ygyno.2010.11.004. Epub 2010 Dec 15. Efemp1 Expression Promotes Angiogenesis And Accelerates The Growth Of Cervical Can...

Lu Zx, Liu Lt, Qi Xr. Int J Nanomedicine. 2011;6:1661-73. Doi: 10.2147/Ijn.S22293. Epub 2011 Aug 11. Development Of Small Interfering Rna Delivery System Using Pei-Peg-Aprpg Polymer For Antiangiogenic Vascular Endothelial Growth Factor Tumor-Targe...

Wang F, Liu S, Sun Q, Zhang L, Song Y, Sheng W, Xi S, Sun G. Occup Environ Med. 2014 Oct;71(10):675-80. Doi: 10.1136/Oemed-2014-102173. Epub 2014 Jul 4. Urinary Vegf And Pge2 Levels And The Association With Arsenical Metabolites In Copper-Smelting...

Zhang Hy, Fan Bl, Wu Xs, Mu Lm, Wang Wf, Zhu Wl. Mol Med Rep. 2015 May;11(5):4010. Doi: 10.3892/Mmr.2015.3178. Epub 2015 Jan 9. [Corrigendum] Overexpression Of The Chimeric Plasmin-Resistant Vegf165/Vegf183 (132-158) Protein In Murine Breast Cance...

Shi J, Liu F, Zhang W, Liu X, Lin B, Tang X. Oncol Rep. 2015 Jun;33(6):2972-80. Doi: 10.3892/Or.2015.3889. Epub 2015 Mar 31. Epigallocatechin-3-Gallate Inhibits Nicotine-Induced Migration And Invasion By The Suppression Of Angiogenesis And Epithel...

Sancakdar E, Guven As, Uysal Eb, Deveci K, G??lt??rk E. Pediatr Infect Dis J. 2015 Aug;34(8):E200-5. Doi: 10.1097/Inf.0000000000000706. Important Of Angiopoietic System In Evaluation Of Endothelial Damage In Children With Crimean-Congo Hemorrhagic...

Lin L, Han Mm, Wang F, Xu Ll, Yu Hx, Yang Py. Cell Death Dis. 2014 Oct 23;5:E1488. Doi: 10.1038/Cddis.2014.392. Cxcr7 Stimulates Mapk Signaling To Regulate Hepatocellular Carcinoma Progression.

Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.

Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ...

He L, Zhang E, Shi J, Li X, Zhou K, Zhang Q, Le Ad, Tang X. Cancer Chemother Pharmacol. 2013 Mar;71(3):713-25. Doi: 10.1007/S00280-012-2063-Z. Epub 2013 Jan 6. (-)-Epigallocatechin-3-Gallate Inhibits Human Papillomavirus (Hpv)-16 Oncoprotein-Induc...

Zhang E, Feng X, Liu F, Zhang P, Liang J, Tang X. Plos One. 2014 Jul 24;9(7):E103440. Doi: 10.1371/Journal.Pone.0103440. Ecollection 2014. Roles Of Pi3K/Akt And C-Jun Signaling Pathways In Human Papillomavirus Type 16 Oncoprotein-Induced Hif-1??, ...

Chen L, Xiao Z, Meng Y, Zhao Y, Han J, Su G, Chen B, Dai J. Biomaterials. 2012 Feb;33(5):1437-44. Doi: 10.1016/J.Biomaterials.2011.10.056. Epub 2011 Nov 10. The Enhancement Of Cancer Stem Cell Properties Of Mcf-7 Cells In 3D Collagen Scaffolds For...

Liu Lj, Zhang J, Xiao Zf, Dai B, Sun My, Chen L, Chen B. J Biomed Mater Res B Appl Biomater. 2014 May;102(4):772-80. Doi: 10.1002/Jbm.B.33058. Epub 2013 Oct 21. Three-Dimensional Collagen Scaffold Enhances The Human Adenoid Cystic Carcinoma Cancer...

Chen J, Xu J, Li J, Du L, Chen T, Liu P, Peng S, Wang M, Song H. Int Immunopharmacol. 2015 May;26(1):147-52. Doi: 10.1016/J.Intimp.2015.03.025. Epub 2015 Mar 31. Epigallocatechin-3-Gallate Attenuates Lipopolysaccharide-Induced Mastitis In Rats Via...

Yang Py, Rui Yc, Lu L, Li Tj, Liu Sq, Yan Hx, Wang Hy. Life Sci. 2005 Sep 30;77(20):2529-39. Time Courses Of Vascular Endothelial Growth Factor And Intercellular Adhesion Molecule-1 Expressions In Aortas Of Atherosclerotic Rats.

Have you used Human VEGF ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human VEGF ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

21 Customer Q&As for Human VEGF ELISA Kit PicoKine®

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of VEGFA in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-10-16

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the VEGFA ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-10-16

Question

Q: Can VEGFA ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-08-25

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-08-25

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Z. Jackson

Verified customer

Asked: 2020-07-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-07-22

Question

I would like to know the epitopes and cross reactivity of this Human VEGF ELISA Kit PicoKine antibody as well as the animal of origin.

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

The Human VEGF ELISA Kit PicoKine, Capture antibody; monoclonal mouse and the immunogen sequence is Ala27

Boster Scientific Support

Answered: 2020-03-23

Question

Q: how to analyze ELISA data? I measured VEGFA level in plasma.

Verified Customer

Verified customer

Asked: 2020-02-09

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-02-09

Question

I want to measure VEGF in urine using ELISA. I saw your product Human VEGF ELISA Kit PicoKine ,is it suitable for urine samples?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

The Human VEGF ELISA Kit PicoKine is suitable in detecting VEGF in biological fluids such as cell culture supernatant, serum and plasma.

Boster Scientific Support

Answered: 2020-01-16

Question

Has your lab ever tested EK0539 in urine samples? Or, have you had a customer that has had success/failure in testing this kit with urine samples?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

Our lab technicians haven't

Boster Scientific Support

Answered: 2019-11-25

Question

What is being used in your method when transfering genes to sf21 cells for EK0539?

Verified customer

Asked: 2019-11-14

Answer

For the Human VEGF ELISA Kit PicoKine™ (EK0539), we use baculovirus to transfer genes to sf21 cells.

Boster Scientific Support

Answered: 2019-11-14

Question

How come I saw no signal for running your kit with my VEGF plasma sample?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

First of all, the amount of VEGF antigen in plasma is much lower than that in serum, and it is possible that the antigen is undetectable by the assay. You may use serum instead of plasma if applicable.

Boster Scientific Support

Answered: 2019-11-13

Question

What is a possible cause for obtaining low data results with EK0539?

Verified customer

Asked: 2019-11-11

Answer

For the Human VEGF ELISA Kit PicoKine® (EK0539), the lower data might be caused by some factors which include but not limited to unstable temperature during transportation and variation of operation conditions.

Boster Scientific Support

Answered: 2019-11-18

Question

Have other isoforms been tested for cross-reactivity with EK0541 and EK0539?

Verified customer

Asked: 2019-09-25

Answer

The The Human VEGF ELISA Kit PicoKine EK0539 and Mouse VEGF ELISA Kit PicoKine EK0541 are cross-reactive with their specific targets only.

Boster Scientific Support

Answered: 2019-09-26

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

P. Dayal

Verified customer

Asked: 2019-08-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-08-10

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification VEGFA using the EK0539 Human VEGFA Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-09-30

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-09-30

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-09-29

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-09-29

Question

Q: how to thaw whole blood sample for VEGFA ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-09-12

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFA for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-09-12

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-06-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-26

Question

Q: if the enzyme conjugated VEGFA antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the VEGFA antigen?

Verified Customer

Verified customer

Asked: 2018-03-23

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-03-23

Question

Does this kit detect all forms of VEGF or just VEGFA

Verified Customer

Verified customer

Asked: 2017-08-02

Answer

This assay detects VEGFA

Boster Scientific Support

Answered: 2017-08-02

Question

I would like to purchase Human VEGF ELISA Kit PicoKine and use it on protein extracts in lysis buffer containing 8M urea. Do you have any information if the ELISA work with Urea 1M after dilution of the sample in diluent buffer?

Verified Customer

Verified customer

Asked: 2017-05-12

Answer

Unfortunately, we have not tested the effects of Urea containing lysis buffer on samples with this antibody Human VEGF ELISA Kit PicoKine, so we do not have any idea how well this would work. But it is important to note that Urea is a Chaotropic agent at 6 - 8 M that means it can denature proteins, thus this buffer formula may unfavorably affect the VEGF protein causing it not to be recognized by the ELISA.

Boster Scientific Support

Answered: 2017-05-12

Question

Can you tell me how to troubleshoot?

Verified Customer

Verified customer

Asked: 2016-09-15

Answer

To troubleshoot, you should try to repeat your experiment without diluting your plasma samples. If this approach still does not work, try to increase your sample incubation time to 2.5 hours (at room temperature). To minimize any potential sample losses, we recommend you pilot your experiment using standards and a small number of samples.

Boster Scientific Support

Answered: 2016-09-15

Question

For the kit EK0539 a customer ask if the kit detects all forms of VEGF or only VEGFA?

Verified Customer

Verified customer

Asked: 2014-02-10

Answer

EK0539 detects just VEGFA.

Boster Scientific Support

Answered: 2014-02-10

$369

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

ELISA Kits

ELISA Kits

More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

Image

Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit

Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product
EK0539
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.