Human BDNF ELISA Kit PicoKine™

Human BDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Human BDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml. Cited in 31 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK0307
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).

Product Name

Human BDNF ELISA Kit PicoKine™

See all Bdnf products

SKU/Catalog Number

EK0307

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human BDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Human BDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human BDNF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0307)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: H129-R247

Sensitivity

<15 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0307 is reactive to BDNF in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0307 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0307-CAP Anti-Human BDNF Pre-coated 96-well strip microplate 1
EK0307-ST Human BDNF Standard 2 vials, 10 ng/tube
EK0307-DA Human BDNF Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0250.0740.1010.1600.2870.5631.2511.916

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human BDNF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)47224102244244926
Standard deviation3.579.6344.963.7812.4450
CV (%)7.6%4.3%4.4%8.6%5.1%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Bdnf in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 147565152513.26.2%
Sample 222418822120420914.446.9%
Sample 310221010870105098869.657%
*number of samples for each test n=16.

Gene/Protein Information For BDNF (Source: Uniprot.Org, NCBI)

Uniprot ID

P23560

Gene ID

627

Gene Name

BDNF

Full Name

Brain-derived neurotrophic factor

Weight

27.818kDa

Superfamily

NGF-beta family

Alternative Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BDNF, check out the BDNF Infographic

BDNF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0307 has been cited in 31 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hogervorst JGF,Saenen ND,Nawrot TS.Gestational acrylamide exposure and biomarkers of fetal growth: Probing the mechanism underlying the association between acrylamide and reduced fetal growth.Environ Int.2021 Jun 11;155:106668.doi:10.1016/j.envint.2021.106668.Epub ahead of print.PMID:34120003.
Species: Human
EK0307 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Ruiz-González D,Hernández-Martínez A,Valenzuela PL,Morales JS,Soriano-Maldonado A.Effects of physical exercise on plasma brain-derived neurotrophic factor in neurodegenerative disorders: A systematic review and meta-analysis of randomized controlled trials.Neurosci Biobehav Rev.2021 Jun 1:S0149-7634(21)00235-9.doi:10.1016/j.neubiorev.2021. 05. 025.Epub ahead of print.PMID:34087277.
Species: Human
EK0307 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Miniksar ÖH,Çiçekçioğlu F,Kılıç M,Honca M,Miniksar DY,Gocmen AY,Kaçmaz O,Öz H.Decreased brain-derived neurotrophic factor levels may predict early perioperative neurocognitive disorder in patients undergoing coronary artery bypass surgery: A prospective observational pilot study.J Clin Anesth.2021 Mar 25;71:110235.doi:10.1016/j. jclinane.2021.11023 5.Epub ahead of print.PMID:33774438.
Species: Human
EK0307 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Ahmed MN,Shi D,Dailey MT,Rothermund K,Drewry MD,Calabrese TC,Cui XT,Syed-Picard FN. Dental pulp cell sheets enhance facial nerve regeneration via local neurotrophic factor delivery. Tissue Eng Part A.2020 Nov 9. doi:10.1089/ten.TEA.2020.0265.Epub ahead of
Species: Human
EK0307 usage in article: APP:ELISA, SAMPLE:DPC SHEETS, DILUTION:NA

Gürgen, Seren Gülşen, et al. "Metoclopramide Use to Induce Lactation Can Alter BDNF and DrD2 in The Prefrontal Cortex of Offspring." Journal of Chemical Neuroanatomy (2020): 101844.

Does the Cerebrospinal Fluid Reflect Altered Redox State But Not Neurotrophic Support Loss in Parkinson's Disease?

BDNF-overexpressing human mesenchymal stem cells mediate increased neuronal protection in vitro

The roles of valerenic acid on BDNF expression in the SH-SY5Y cell.

Application of bFGF and BDNF to improve angiogenesis and cardiac function.

Cytokine, chemokine and BDNF levels in medication-free pediatric patients with obsessive-compulsive disorder.

Burnout and cognitive impairment: Associated with serum BDNF in a Chinese Han population

Altered levels of brain-derived neurotrophic factor, proBDNF and tissue plasminogen activator in children with posttraumatic stress disorder.

Neurotherapeutic Effect of Cord Blood Derived CD45 Hematopoietic Cells in Mice after Traumatic Brain Injury

Research on changes in cognitive function, %u03B2-amyloid peptide and neurotrophic factor in stroke patients.

The Neuroprotective effects of muscle-derived stem cells via brain-derived neurotrophic factor in spinal cord injury model

Lithium protects against paraquat neurotoxicity by NRF2 activation and miR-34a inhibition in SH-SY5Y cells

Brain-derived neurotrophic factor promotes human granulosa-like tumor cell steroidogenesis and proliferation by activating the FSH receptor-mediated signaling pathway

Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear

Neurotherapeutic Effect of Cord Blood Derived CD45+?Hematopoietic Cells in Mice after Traumatic Brain Injury

Maiti R, Mishra BR, Jowhar J, Mohapatra D, Parida S, Bisoi D. Clin Psychopharmacol Neurosci. 2017 May 31;15(2):170-176. doi: 10.9758/cpn.2017.15.2.170. Effect of Oxcarbazepine on Serum Brain Derived Neurotrophic Factor in Bipolar Mania: An Explora...

Erpolat S, Celik HT, Bozkurt B. Postepy Dermatol Alergol. 2017 Aug;34(4):346-349. doi: 10.5114/ada.2017.69315. Epub 2017 Aug 2. Brain-derived neurotrophic factor is increased in serum levels of patients with symptomatic dermographism

Bakirhan A, Yalcin Sahiner S, Sahiner IV, Safak Y, Goka E. PLoS One. 2017 Dec 29;12(12):e0189373. doi: 10.1371/journal.pone.0189373. eCollection 2017. Association of serum brain derived neurotropic factor with duration of drug-naive period and pos...

Schmidt N, Schulze J, Warwas DP, Ehlert N, Lenarz T, Warnecke A, Behrens P. PLoS One. 2018 Mar 27;13(3):e0194778. doi: 10.1371/journal.pone.0194778. eCollection 2018. Long-term delivery of brain-derived neurotrophic factor (BDNF) from nanoporous s...

Köse Çinar, R. (2017). Telomere length and hTERT in mania and subsequent remission. Revista Brasileira de Psiquiatria. Advance online publication. doi: 10.1590/1516-4446-2017-2216

Schulze, J., Kaiser, O., Paasche, G., Lamm, H., Pich, A., Hoffmann, A.,..., & Warnecke, A. (2017). Effect of hyperbaric oxygen on BDNF-release and neuroprotection: Investigations with human mesenchymal stem cells and genetically modified NIH3T3 fi...

Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.

Forsyth Pa, Krishna N, Lawn S, Valadez Jg, Qu X, Fenstermacher Da, Fournier M, Potthast L, Chinnaiyan P, Gibney Gt, Zeinieh M, Barker Pa, Carter Bd, Cooper Mk, Kenchappa Rs. J Biol Chem. 2014 Mar 21;289(12):8067-85. Doi: 10.1074/Jbc.M113.513762. E...

Tajerian M, Leu D, Zou Y, Sahbaie P, Li W, Khan H, Hsu V, Kingery W, Huang Tt, Becerra L, Clark Jd. Anesthesiology. 2014 Oct;121(4):852-65. Doi: 10.1097/Aln.0000000000000403. Brain Neuroplastic Changes Accompany Anxiety And Memory Deficits In A Mo...

Shi Y, You J, Yuan Y, Zhang X, Li H, Hou G. Psychiatry Clin Neurosci. 2010 Jun;64(3):249-54. Doi: 10.1111/J.1440-1819.2010.02074.X. Plasma Bdnf And Tpa Are Associated With Late-Onset Geriatric Depression.

Teche Sp, Nuernberg Gl, Sordi Ao, De Souza Lh, Remy L, Ceres??r Km, Rocha Ns. Psychiatr Q. 2013 Dec;84(4):485-97. Doi: 10.1007/S11126-013-9261-7. Measurement Methods Of Bdnf Levels In Major Depression: A Qualitative Systematic Review Of Clinical T...

Zakharyan R, Boyajyan A. Clin Biochem. 2014 Aug;47(12):1052-5. Doi: 10.1016/J.Clinbiochem.2014.03.021. Epub 2014 Apr 5. Brain-Derived Neurotrophic Factor Blood Levels Are Decreased In Schizophrenia Patients And Associate With Rs6265 Genotypes.

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1 Reviews For Human BDNF ELISA Kit PicoKine™

0

"Quantification of BDNF Post-Transfection in Primary Neuronal Cells"

Excellent

Source: Biocompare.com

"Quantification of BDNF Post-Transfection in Primary Neuronal Cells"

--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student

Application ELISA
Sample Cell lysate and culture medium
Detection --

"The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.")

Verified customer

Submitted

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13 Customer Q&As for Human BDNF ELISA Kit PicoKine™

Question

What isoform does EK0307 detect?

Verified customer

Asked: 2020-11-16

Answer

The Human BDNF ELISA Kit PicoKine™ (EK0307) detects total BDNF.

Boster Scientific Support

Answered: 2020-11-16

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Bdnf using the EK0307 Human Bdnf Picokine® ELISA Kit?

B. Gandhi

Verified customer

Asked: 2020-04-03

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-04-03

Question

Will this ELISA kit work on saliva or nasal washes?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

We have not validated this kit on saliva or nasal washes.It can be detected if the concentration of BDNF in saliva or nasal wash samples are in the detection range of the kit.

Boster Scientific Support

Answered: 2020-03-06

Question

My samples have highly concentrated levels of BDNF and require more than normal dilution. Can you provide any extra (~ 90 mL) sample diluent? Do you sell the diluent separately?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

Yes, we sell the diluent separately (Boster Catalog# AR1106-1). In fact, the sample diluent buffer in the kit is generally enough. For instance, if the dilution ratio is 1:20000, it is not necessary to dilute the samples at 1:20000 in just one step. Instead, we suggest you sequentially dilute the samples: 1:100, 1.200, etc. That way, you can use less diluent buffer.

Boster Scientific Support

Answered: 2020-02-12

Question

Which isoform (mature or precursor) does this ELISA assay detect? I am also wondering if the dimer form of mature BDNF can be detection by ELISA.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

The assay recognizes both of mature and precursor isoforms of BDNF and can detect the dimer form. So, this detects total BDNF.

Boster Scientific Support

Answered: 2019-12-23

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-09-17

Question

I am interested in performing Western Blot (WB) using the antibodies from your human and mouse BDNF ELISA kits. Is it possible to order the primary antibody (the capture antibody) that is pre-coated in the wells for the sandwich ELISA? (In this case that would be the monoclonal antibody from rat for the mouse kit, and the monoclonal antibody from mouse for the human kit). Is it also possible to order your secondary antibody which is the biotinylated detection polyclonal antibody from goat specific for BDNF?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

The antibodies used in those ELISA kits are not fully validated on WB. Also, since these antibodies are made from recombinant proteins, they are often less reactive to retrieved antigens in WB or IHC due to the lack of tertiary structure in the retrieved antigens. If you want to test human and mouse BDNF with WB, please use our anti-BDNF antibody (PB9075). Further, you can separately buy our secondary antibodies.

Boster Scientific Support

Answered: 2019-08-28

Question

Is the BDNF measured by this Human BDNF ELISA Kit PicoKine a mature BDNF or pro-BDNF? What is the conversion factor to calculate the concentration in moles?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

The capture and detection used in this Human BDNF ELISA Kit PicoKine antibodies are both recognizes the region within Arg128

Boster Scientific Support

Answered: 2019-06-21

Question

Hello Support: I would like to ask if this antibody Human BDNF ELISA Kit PicoKine would quantify and detect BDNF in cerebrospinal fluid, not only in plasma or cell culture.

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

The product Human BDNF ELISA Kit PicoKine has not been tested in cerebrospinal fluid, it is only validated in with plasma, serum and cell culture, however, this kit is very accommodating to many different samples types, so cerebrospinal fluid will most likely work

Boster Scientific Support

Answered: 2019-06-14

Question

Q: Can Bdnf ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Puri

Verified customer

Asked: 2019-01-24

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-01-24

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

M. Jones

Verified customer

Asked: 2016-03-20

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-03-20

Question

What is the expression system for the standards? Do the standards contain carrier protein? Keywords: product immunogen information, antigen sequence, expression standard

Verified Customer

Verified customer

Asked: 2014-04-28

Answer

The expression system of the standards can be found in the product page under "

Boster Scientific Support

Answered: 2014-04-28

Question

Does EK0307 have any cross-reactivity with other neurotrophins, especially proBDNF?

Verified Customer

Verified customer

Asked: 2014-03-12

Answer

EK0307 is able to detect total BDNF. It has no significant cross-reactivity or interference with the following factors: BDNF (pro-domain, aa 19-128), NGF-beta, NGFR, NT-3, NT-4, TrkA, and TrkB.

Boster Scientific Support

Answered: 2014-03-12

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