Epoxide hydrolase (EPHX1) (NM_000120) Human Over-expression Lysate

Lysate of EPHX1 over-expression cell lines, WB control

Transient overexpression lysate of epoxide hydrolase 1, microsomal (xenobiotic) (EPHX1), transcript variant 1

Product Info Summary

SKU: LS002052
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of epoxide hydrolase 1, microsomal (xenobiotic) (EPHX1), transcript variant 1

Product Name

Epoxide hydrolase (EPHX1) (NM_000120) Human Over-expression Lysate

See all EPHX1 products

SKU/Catalog Number

LS002052

Size

100 µg

Description

Transient overexpression lysate of epoxide hydrolase 1, microsomal (xenobiotic) (EPHX1), transcript variant 1

Storage & Handling

Cite This Product

Epoxide hydrolase (EPHX1) (NM_000120) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS002052)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for EPHX1 (Source: Uniprot.org, NCBI)

Uniprot ID

P07099

Gene ID

2052

Gene Name

EPHX1

Full Name

Epoxide hydrolase 1

Weight

52.949kDa

Superfamily

peptidase S33 family

Alternative Names

EC 3.3.2.9; EPHX; EPHX1; EPOX; Epoxide Hydratase; Epoxide Hydrolase 1; epoxide hydrolase 1, microsomal (xenobiotic); HYL1; MEH; MEH8; Microsomal Epoxide Hydrolase EPHX1 EPHX, EPOX, HYL1, MEH epoxide hydrolase 1 epoxide hydrolase 1|epoxide hydratase|epoxide hydrolase 1 microsomal|epoxide hydrolase 1, microsomal (xenobiotic)

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on EPHX1, check out the EPHX1 Infographic

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