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Product Info Summary
| SKU: | LS002099 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of estrogen receptor 1 (ESR1), transcript variant 1 |
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Product info
Product Name
Estrogen Receptor 1 (ESR1) (NM_000125) Human Over-expression Lysate
See all ER alpha/NR3A1 products
SKU/Catalog Number
LS002099
Size
100 µg
Description
Transient overexpression lysate of estrogen receptor 1 (ESR1), transcript variant 1
Storage & Handling
Cite This Product
Estrogen Receptor 1 (ESR1) (NM_000125) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS002099)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Estrogen Receptor 1 (ESR1) Human Over-expression Lysates NM_000125.
Western blot validation of overexpression lysate (LS002099) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ESR1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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Estrogen Receptor 1 (ESR1) Human Over-expression Lysates NM_001122740.
Western blot validation of overexpression lysate (LS002099) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ESR1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ESR1 (Source: Uniprot.org, NCBI)
Gene Name
ESR1
Full Name
Estrogen receptor
Weight
66.216kDa
Superfamily
nuclear hormone receptor family
Alternative Names
ER alpha; ER; Era; ER-alpha; ESR1; ESRESRA; Estradiol receptor; estrogen receptor 1; estrogen receptor alpha delta 3*4,56,7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*5,6,7*/654 isoform; estrogen receptor alpha; estrogen receptor; NR3A1; NR3A1DKFZp686N23123; Nuclear receptor subfamily 3 group A member 1 ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ESR1, check out the ESR1 Infographic
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In this infographic, you will see the following information for ESR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Estrogen Receptor 1 (ESR1) (NM_000125) Human Over-expression Lysate (LS002099)
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No publications found for LS002099
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