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Product Info Summary
|Description:||Transient overexpression lysate of sulfotransferase family 1E, estrogen-preferring, member 1 (SULT1E1)|
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Estrogen Sulfotransferase (SULT1E1) (NM_005420) Human Over-expression Lysate
Transient overexpression lysate of sulfotransferase family 1E, estrogen-preferring, member 1 (SULT1E1)
Storage & Handling
Cite This Product
Estrogen Sulfotransferase (SULT1E1) (NM_005420) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006783)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Estrogen Sulfotransferase (SULT1E1) Human Over-expression Lysates NM_005420.
Western blot validation of overexpression lysate (LS006783) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SULT1E1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SULT1E1 (Source: Uniprot.org, NCBI)
sulfotransferase 1 family
Cytosolic Sulfotransferase 1E1; EC 2.8.2; EST-1; ESTMGC34459; estrone sulfotransferase; ST1E1; STE; STEestrogen sulfotransferase; Sulfotransferase 1E1; sulfotransferase family 1E, estrogen-preferring, member 1; Sulfotransferase, estrogen-preferringEC 22.214.171.124; SULT1E1 SULT1E1 EST, EST-1, ST1E1, STE sulfotransferase family 1E member 1 sulfotransferase 1E1|estrogen sulfotransferase|estrone sulfotransferase|sulfotransferase family 1E, estrogen-preferring, member 1|sulfotransferase, estrogen-preferring*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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Specific Publications For Estrogen Sulfotransferase (SULT1E1) (NM_005420) Human Over-expression Lysate (LS006783)
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