ETK (Phospho-Tyr40) Colorimetric Cell-Based ELISA Kit

BMX ELISA kit, phospho-specific, cell-based

The ETK (Phospho-Tyr40) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ETK (Phospho-Tyr40) protein expression profile in cells. The kit can be used for measuring the relative amounts of ETK (Phospho-Tyr40) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ETK (Phospho-Tyr40).

Product Info Summary

SKU: EKC2153
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA
Sample Types: Cell lines

Product Name

ETK (Phospho-Tyr40) Colorimetric Cell-Based ELISA Kit

See all BMX products

SKU/Catalog Number

EKC2153

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The ETK (Phospho-Tyr40) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ETK (Phospho-Tyr40) protein expression profile in cells. The kit can be used for measuring the relative amounts of ETK (Phospho-Tyr40) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ETK (Phospho-Tyr40).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

ETK (Phospho-Tyr40) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2153)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2153 is reactive to BMX in Human, Mouse samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2153 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-ETK Phospho-Tyr40 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-ETK Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For BMX (Source: Uniprot.Org, NCBI)

Gene Name

BMX

Full Name

Cytoplasmic tyrosine-protein kinase BMX

Weight

78011 MW

Superfamily

protein kinase superfamily

Alternative Names

BMX;BMX NON-receptor tyrosine kinase;Bone marrow kinase BMX;Cytoplasmic BMX;Epithelial and endothelial tyrosine kinase;NTK38;Protein tyrosine kinase BMX;kinase Etk BMX ETK, PSCTK2, PSCTK3 BMX non-receptor tyrosine kinase cytoplasmic tyrosine-protein kinase BMX|BTK-like on X chromosome|Etk/Bmx cytosolic tyrosine kinase|NTK38 tyrosine kinase|bone marrow tyrosine kinase gene in chromosome X protein|epithelial and endothelial tyrosine kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BMX, check out the BMX Infographic

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