FAM108A1 (ABHD17A) (NM_031213) Human Over-expression Lysate

Lysate of FAM108A1 over-expression cell lines, WB control

Transient overexpression lysate of family with sequence similarity 108, member A1 (FAM108A1), transcript variant 1

Product Info Summary

SKU: LS081926
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of family with sequence similarity 108, member A1 (FAM108A1), transcript variant 1

Product Name

FAM108A1 (ABHD17A) (NM_031213) Human Over-expression Lysate

See all FAM108A1 products

SKU/Catalog Number

LS081926

Size

100 µg

Description

Transient overexpression lysate of family with sequence similarity 108, member A1 (FAM108A1), transcript variant 1

Storage & Handling

Cite This Product

FAM108A1 (ABHD17A) (NM_031213) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081926)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for ABHD17A (Source: Uniprot.org, NCBI)

Gene Name

ABHD17A

Full Name

Alpha/beta hydrolase domain-containing protein 17A

Weight

33.99kDa

Superfamily

AB hydrolase superfamily

Alternative Names

abhydrolase domain-containing protein FAM108A1; C19orf27; chromosome 19 open reading frame 27; EC 3.-; family with sequence similarity 108, member A1; MGC5244 ABHD17A C19orf27, FAM108A1 abhydrolase domain containing 17A, depalmitoylase alpha/beta hydrolase domain-containing protein 17A|abhydrolase domain-containing protein 17A|abhydrolase domain-containing protein FAM108A1|family with sequence similarity 108, member A1|protein ABHD17A

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on ABHD17A, check out the ABHD17A Infographic

ABHD17A infographic

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In this infographic, you will see the following information for ABHD17A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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