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Product Info Summary
|Description:||Transient overexpression lysate of family with sequence similarity 167, member A (FAM167A)|
FAM167A (NM_053279) Human Over-expression Lysate
Transient overexpression lysate of family with sequence similarity 167, member A (FAM167A)
Storage & Handling
Cite This Product
FAM167A (NM_053279) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083648)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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FAM167A Human Over-expression Lysates NM_053279.
Western blot validation of overexpression lysate (LS083648) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM167A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for FAM167A (Source: Uniprot.org, NCBI)
FAM167 (SEC) family
Protein FAM167A FAM167A C8orf13, D8S265, DIORA-1 family with sequence similarity 167 member A protein FAM167A|disordered autoimmunity 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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