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Product Info Summary
|Description:||Transient overexpression lysate of family with sequence similarity 186, member B (FAM186B), transcript variant 1|
FAM186B (NM_032130) Human Over-expression Lysate
Transient overexpression lysate of family with sequence similarity 186, member B (FAM186B), transcript variant 1
Storage & Handling
Cite This Product
FAM186B (NM_032130) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084070)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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FAM186B Human Over-expression Lysates NM_032130.
Western blot validation of overexpression lysate (LS084070) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM186B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for FAM186B (Source: Uniprot.org, NCBI)
C12orf25; chromosome 12 open reading frame 25; DKFZp434J0113; DKFZp434N2350; family with sequence similarity 186, member B; hypothetical protein LOC84070; protein FAM186B FAM186B C12orf25 family with sequence similarity 186 member B protein FAM186B*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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