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Product Info Summary
|Description:||Transient overexpression lysate of pyroglutamylated RFamide peptide receptor (QRFPR)|
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GPR103 (QRFPR) (NM_198179) Human Over-expression Lysate
Transient overexpression lysate of pyroglutamylated RFamide peptide receptor (QRFPR)
Storage & Handling
Cite This Product
GPR103 (QRFPR) (NM_198179) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084109)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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GPR103 (QRFPR) Human Over-expression Lysates NM_198179.
Western blot validation of overexpression lysate (LS084109) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with QRFPR (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for QRFPR (Source: Uniprot.org, NCBI)
Pyroglutamylated RF-amide peptide receptor
G-protein coupled receptor 1 family
AQ27; G protein-coupled receptor 103; GPR103; G-protein coupled receptor 103; MGC149217; Orexigenic neuropeptide QRFP receptor; pyroglutamylated RFamide peptide receptor; QRFP receptor; QRFPR; SP9155 QRFPR AQ27, GPR103, SP9155 pyroglutamylated RFamide peptide receptor pyroglutamylated RF-amide peptide receptor|G-protein coupled receptor 103|QRFP receptor|orexigenic neuropeptide QRFP receptor|peptide P518 receptor*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on QRFPR, check out the QRFPR Infographic
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In this infographic, you will see the following information for QRFPR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS084109
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