Horse equine TGF-Beta 3 ELISA Kit PicoKine®

TGF-beta 3 ELISA kit for Equine

Horse equine TGF-Beta 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Horse TGFB3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1103-EQ
Size: 96 wells/kit, with removable strips.
Reactive Species: Equine
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (EDTA) and urine.

Product Name

Horse equine TGF-Beta 3 ELISA Kit PicoKine®

View all TGF-beta 3 ELISA kits

SKU/Catalog Number

EK1103-EQ

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Horse equine TGF-Beta 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Horse TGFB3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Horse equine TGF-Beta 3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1103-EQ)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A346-S457

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1103-EQ for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1103-EQ is reactive to TGFB3 in Equine samples

Validated Sample Types

cell culture supernatants, serum, plasma (EDTA) and urine.

Application Guarantee

EK1103-EQ is guaranteed for ELISA in Equine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TGF-beta 3

Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.

Kit Components

Catalog Number Description Quantity
EK1103-EQ-CAP Anti-Equine TGFB3 Pre-coated 96-well strip microplate 1
EK1103-EQ-ST Equine TGFB3 Standard 2 vials, 10 ng/tube
EK1103-EQ-DA Equine TGFB3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.2562.512525050010002000
O.D.0.0920.1750.2170.3020.5040.8121.3211.984

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Horse equine TGF-Beta 3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)27163111532946701265
Standard deviation14.4294518.8236.472.85
CV (%)5.34.63.96.45.75.8

Gene/Protein Information For TGFB3 (Source: Uniprot.Org, NCBI)

Gene Name

TGFB3

Full Name

Transforming growth factor beta-3 proprotein

Weight

47.328kDa

Superfamily

TGF-beta family

Alternative Names

ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3 TGFB3 ARVD, ARVD1, LDS5, RNHF, TGF-beta3 transforming growth factor beta 3 transforming growth factor beta-3 proprotein|prepro-transforming growth factor beta-3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TGFB3, check out the TGFB3 Infographic

TGFB3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TGFB3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Horse equine TGF-Beta 3 ELISA Kit PicoKine®

Question

What is the shelf life of the EK1103-EQ kit?

Verified customer

Asked: 2022-04-19

Answer

The Horse Equine TGF-Beta 3 ELISA Kit PicoKine® (EK1103-EQ) could be stored at 4°C for 6 months, at -20°C for 12 months.

Boster Scientific Support

Answered: 2022-04-19

Question

Q: how do I analyze ELISA data? I have obtained TGF-beta 3 level in serum.

H. Kumar

Verified customer

Asked: 2020-07-31

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-07-31

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-09-17

Question

Q: Can TGF-beta 3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

L. Williams

Verified customer

Asked: 2019-07-01

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-07-01

Question

Q: is it okay to use edta plasma as samples in Horse TGF-beta 3 Picokine® ELISA Kit (Catalog # EK1103-EQ)?

Verified Customer

Verified customer

Asked: 2018-12-07

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TGF-beta 3 causing degradation of its protein structure. TGF-beta 3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TGF-beta 3 ELISA, treating samples with different anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-12-07

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-09-20

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-09-20

Question

Q: can you suggest the dilution ratio of serum samples for detection of TGF-beta 3 in Horse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-01-17

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGF-beta 3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-01-17

Question

Q: how can I thaw whole blood sample for TGF-beta 3 ELISA after freezing?

M. Young

Verified customer

Asked: 2016-09-27

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGF-beta 3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-09-27

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