HRP Conjugated Avidin

Avidin is a 68KD protein that extracted from egg white. It has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Cited in 10 publication(s).

Product Info Summary

SKU: BA1081
Size: 0.5ml
Application: ELISA, IHC, WB

Product NameHRP Conjugated Avidin
SKU/Catalog NumberBA1081
Pack Size0.5 ml
FormConcentrated, Liquid
Concentration1mg/ml
Product DescriptionAvidin is a 68KD protein that extracted from egg white. It has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction.
Contents0.5mg of HRP conjugated avidin; 0.01 M PBS (pH7.4), 50% glycerol.
Labeling MethodAvidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
(Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)
StorageAt -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
ApplicationsWB, IHC, ELISA
Cite This ProductPeroxidase Conjugated Avidin (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1081)

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blotting: 0.25-0.5μg/ml (ECL detection) Western Blotting: 2.5-5μg/ml (DAB detection) Immunohistochemistry: 2-10μg/ml Direct ELISA: 0.01-0.15μg/ml (TMB detection)

Validation Images & Assay Conditions

Hello CJ!

BA1081 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of salinomycin and 17‑AAG on proliferation of human gastric cancer cells in vitro

Effects of survivin on FVADT chemotherapy for refractory multiple myeloma

Investigation of the protective effect of erythropoietin on spinal cord injury in rats

Oral Resveratrol Prevents Osteoarthritis Progression in C57BL/6J Mice Fed a High-Fat Diet

Amphiphilic core–shell nanoparticles containing dense polyethyleneimine shells for efficient delivery of microRNA to Kupffer cells

Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs

Protective effects of erythropoietin in experimental spinal cord injury by reducing the C/EBP-homologous protein expression

Short-term toxicity of dibutyl phthalate to mice intestinal tissue:

Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute Myocardial Infarction Rat Model

Cloning and characterization of NAD-dependent protein deacetylase (Rv1151c) from Mycobacterium tuberculosis

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1 Customer Q&As for HRP Conjugated Avidin

Question

Can BA1081 be used in Western Blot and detected by chemiluminescence?

Verified customer

Asked: 2021-11-11

Answer

Yes, the HRP Conjugated Avidin (BA1081) can be used in Western Blot and detected by chemiluminescence.

Boster Scientific Support

Answered: 2021-11-12

Size

Total: $95

SKU:BA1081

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