HRP Conjugated AffiniPure Goat Anti-Human IgM

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for human IgM. Cited in 6 publication(s).

Product Info Summary

SKU: BA1077
Size: 0.25ml
Reactive Species: Human
Host: Goat
Application: ELISA, WB

Product Overview

Product Name HRP Conjugated AffiniPure Goat Anti-Human IgM
Synonyms HRP-conjugated goat anti-human IgM
Description HRP Conjugated Goat Anti-human IgM (u-chain specific) secondary antibody This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.
Reagent Type Secondary antibody, reporter enzyme labeled
Label HRP (Horseradish Peroxidase)
Host Goat
Target Species Human
Antibody Class IgM
Clonality Polyclonal
Immunogen Human IgM (whole molecular)
Purification The antibody was purified from antisera by immunoaffinity chromatography.
Specificity This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.
Form Supplied Concentrated, Liquid
Formulation 0.25 mg of HRP conjugated specific antibody
0.01 M PBS (pH 7.4)
50% glycerol
Pack Size 0.25 ml
Concentration 1mg/ml
Storage At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
Application ELISA*, WB*
*Our Boster Guarantee covers the use of this product in the above marked tested applications.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Protein detection/quantification
Technique Indirect immunodetection of target antibody with HRP reporter enzyme
Equipment Needed WB/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
Compatibility with Reagents Incompatible with sodium azide and metals
incompatible with high phosphate concentrations

Main Advantages

Specific High signal-to-noise ratio
Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast Generates strong signals in a relatively short time span
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) Direct ELISA: 0.05-0.5μg/ml (TMB detection)

Validation Images & Assay Conditions

BA1077 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of Ureaplasma urealyticum lipid-associated membrane proteins on rheumatoid arthritis synovial fibroblasts:

Modeling of Diseases of Retinal Ischemia in vitro: Possible Participation of Autocrine Vascular Endothelial Growth Factor Signaling

Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis

Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis

Zeng Z, Lan J, Lei S, Yang Y, He Z, Xue Y, Chen T. Simultaneous Inhibition of Ornithine Decarboxylase 1 and Pyruvate Kinase M2 Exerts Synergistic Effects Against Hepatocellular Carcinoma Cells. Onco Targets Ther. 2020 Nov 17;13:11697-11709. doi: 10.2147/O
Species: Human
BA1077 usage in article: APP, IHC, SAMPLE:HCC CELLS, DILUTION:NA

Molecular characterization of cathepsin B from

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1 Customer Q&As for HRP Conjugated AffiniPure Goat Anti-Human IgM

Question

What is the concentration of BA1077?

Verified customer

Asked: 2019-05-28

Answer

The concentration for the Goat Anti-Human IgM Secondary Antibody, HRP Conjugate (BA1077) is 1mg/ml.

Boster Scientific Support

Answered: 2019-05-28

Size

Total: $80

SKU:BA1077

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