HRP Conjugated Protein A

Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A.
Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including Western blotting and ELISA applications. Cited in 10 publication(s).

Product Info Summary

SKU: BA1080
Size: 0.5ml
Application: ELISA, WB

Product NameHRP Conjugated Protein A
SKU/Catalog NumberBA1080
Pack Size0.5ml
FormConcentrated, Liquid
Concentration1mg/mL
DescriptionProtein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A.
Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including Western blotting and ELISA applications.
Contents0.5 mg of HRP conjugated protein A, 0.01 M PBS (pH7.4), 50% glycerol.
Labeling MethodProtein A is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
(Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)
Storage & ExpirationAt -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
ApplicationsELISA, WB
Cite This ProductPeroxidase Conjugated Protein A (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1080)

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blotting: 0.1-0.3μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) Direct ELISA: 0.1-0.5μg/ml (TMB detection)

Validation Images & Assay Conditions

Hello CJ!

BA1080 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Development of an indirect enzyme-linked immunosorbent assay (ELISA) assay based on a recombinant truncated VP2 (tVP2) protein for the detection of canine parvovirus antibodies

Effect of the Nrf2‑ARE signaling pathway on biological characteristics and sensitivity to sunitinib in renal cell carcinoma

Cyclooxygenase‑2 induces angiogenesis in pancreatic cancer mediated by prostaglandin E2

Hesperetin alleviates renal interstitial fibrosis by inhibiting tubular epithelial‑mesenchymal transition in vivo and in vitro

Identification and characterization of a Streptococcus equi ssp . zooepidemicus immunogenic GroEL protein involved in biofilm formation

The expression and construction of engineering Escherichia coli producing humanized AluY RNAs

Immunoproteomic analysis of bacterial proteins of Actinobacillus pleuropneumoniae serotype 1

Bioactivity, pharmacokinetics, and immunogenicity assays in preclinical and clinical trials for recombinant human endostatin1

Identification of immunoreactive proteins of Streptococcus agalactiae isolated from cultured tilapia in China

Identification of B-cell epitopes in the NSP1 protein of porcine reproductive and respiratory syndrome virus

Have you used HRP Conjugated Protein A?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review HRP Conjugated Protein A

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for HRP Conjugated Protein A

Size

Total: $135

SKU:BA1080

Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
Eddy test
In stock
Order Product
BA1080
$135.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.