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Product Info Summary
| SKU: | LS003312 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of heat shock 70kDa protein 8 (HSPA8), transcript variant 1 |
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Product info
Product Name
Hsc70 (HSPA8) (NM_006597) Human Over-expression Lysate
See all HSPA8/HSC71/Hsc70 products
SKU/Catalog Number
LS003312
Size
100 µg
Description
Transient overexpression lysate of heat shock 70kDa protein 8 (HSPA8), transcript variant 1
Storage & Handling
Cite This Product
Hsc70 (HSPA8) (NM_006597) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003312)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Hsc70 (HSPA8) Human Over-expression Lysates NM_006597.
Western blot validation of overexpression lysate (LS003312) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with HSPA8 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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Hsc70 (HSPA8) Human Over-expression Lysates NM_153201.
Western blot validation of overexpression lysate (LS003312) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with HSPA8 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for HSPA8 (Source: Uniprot.org, NCBI)
Gene Name
HSPA8
Full Name
Heat shock cognate 71 kDa protein
Weight
70.898kDa
Superfamily
heat shock protein 70 family
Alternative Names
Heat shock 70 kDa protein 8; heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate protein 54; heat shock cognate protein, 71-kDa; HSC54; HSC70; HSC70heat shock 70kd protein 10; HSC71; HSP71; HSP73; HSP73constitutive heat shock protein 70; HSPA10; HSPA8; LAP1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71 HSPA8 HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71 heat shock protein family A (Hsp70) member 8 heat shock cognate 71 kDa protein|LPS-associated protein 1|N-myristoyltransferase inhibitor protein 71|constitutive heat shock protein 70|epididymis luminal protein 33|epididymis secretory sperm binding protein Li 72p|heat shock 70kDa protein 8|heat shock 70kd protein 10|heat shock cognate protein 54|lipopolysaccharide-associated protein 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on HSPA8, check out the HSPA8 Infographic
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Specific Publications For Hsc70 (HSPA8) (NM_006597) Human Over-expression Lysate (LS003312)
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