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Product Info Summary
|Description:||Transient overexpression lysate of hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1|
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HSD3B7 (NM_025193) Human Over-expression Lysate
Transient overexpression lysate of hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1
Storage & Handling
Cite This Product
HSD3B7 (NM_025193) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080270)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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HSD3B7 Human Over-expression Lysates NM_025193.
Western blot validation of overexpression lysate (LS080270) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with HSD3B7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for HSD3B7 (Source: Uniprot.org, NCBI)
3 beta-hydroxysteroid dehydrogenase type 7
3 beta-hydroxy-delta 5-C27-steroid oxidoreductase; 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 7-alpha-diol 3-beta-dehydrogenase; C(27) 3-beta-HSD; C(27)-3BETA-HSD; Cholest-5-ene-3-beta; EC 1.1.1.-; EC 18.104.22.168; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7,3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase; PFIC4; SDR11E3; short chain dehydrogenase/reductase family 11E, member 3 HSD3B7 CBAS1, PFIC4, SDR11E3 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 3 beta-hydroxysteroid dehydrogenase type 7|3 beta-hydroxy-delta 5-C27-steroid oxidoreductase|3 beta-hydroxysteroid dehydrogenase type VII|3-beta-HSD VII|3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase|C(27)-3BETA-HSD|c(27) 3-beta-HSD|cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase|short chain dehydrogenase/reductase family 11E, member 3*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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