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Product Info Summary
| SKU: | LS001995 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) (ELAVL3), transcript variant 1 |
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Product info
Product Name
HuC (ELAVL3) (NM_001420) Human Over-expression Lysate
SKU/Catalog Number
LS001995
Size
100 µg
Description
Transient overexpression lysate of ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) (ELAVL3), transcript variant 1
Storage & Handling
Cite This Product
HuC (ELAVL3) (NM_001420) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001995)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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HuC (ELAVL3) Human Over-expression Lysates NM_001420.
Western blot validation of overexpression lysate (LS001995) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ELAVL3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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HuC (ELAVL3) Human Over-expression Lysates NM_032281.
Western blot validation of overexpression lysate (LS001995) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ELAVL3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ELAVL3 (Source: Uniprot.org, NCBI)
Gene Name
ELAVL3
Full Name
ELAV-like protein 3
Weight
39.547kDa
Superfamily
RRM elav family
Alternative Names
DKFZp547J036; ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV-like protein 3; Hu antigen C; Hu-antigen C; HuC; HUCL; HUCParaneoplastic cerebellar degeneration-associated antigen; MGC20653; PLE21Paraneoplastic limbic encephalitis antigen 21 ELAVL3 HUC, HUCL, PLE21 ELAV like RNA binding protein 3 ELAV-like protein 3|ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C)|ELAV like neuron-specific RNA binding protein 3|Hu antigen C|hu-antigen C|paraneoplastic cerebellar degeneration-associated antigen|paraneoplastic limbic encephalitis antigen 21
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ELAVL3, check out the ELAVL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELAVL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For HuC (ELAVL3) (NM_001420) Human Over-expression Lysate (LS001995)
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