Human ACHE ELISA Kit PicoKine®

Acetylcholinesterase/ACHE ELISA kit for Human

Human ACHE ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACHE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2137
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human ACHE ELISA Kit PicoKine®

View all Acetylcholinesterase/ACHE ELISA kits

SKU/Catalog Number

EK2137

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ACHE ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACHE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ACHE ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2137)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: E32-L614

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2137 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2137 is reactive to ACHE in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2137 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Acetylcholinesterase/ACHE

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion.

Kit Components

Catalog Number Description Quantity
EK2137-CAP Anti-Human ACHE Pre-coated 96-well strip microplate 1
EK2137-ST Human ACHE Standard 2 vials, 10 ng/tube
EK2137-DA Human ACHE Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)062.51252505001,0002,0004,000
O.D.0.0600.1520.2330.3580.5940.8831.4022.035

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Acetylcholinesterase/ACHE, Dilution ratio of 1:10, concentration in serum and plasma is around 500 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ACHE ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)4616327852178305
Standard deviation2.077.669.172.710.3215.86
CV(%)4.5%4.7%3.3%5.2%5.8%5.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Acetylcholinesterase/ACHE in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 146513959491.613.3%
Sample 21631881741501697.764.6%
Sample 327831233332931318.475.9%
*number of samples for each test n=16.

Gene/Protein Information For ACHE (Source: Uniprot.Org, NCBI)

Gene Name

ACHE

Full Name

Acetylcholinesterase

Weight

67.796kDa

Superfamily

type-B carboxylesterase/lipase family

Alternative Names

acetylcholinesterase (Yt blood group); Acetylcholinesterase; ACHE; apoptosis-related acetylcholinesterase; ARACHE; EC 3.1.1; EC 3.1.1.7; N-ACHE; Yt blood group; YT ACHE ACEE, ARACHE, N-ACHE, YT acetylcholinesterase (Cartwright blood group) acetylcholinesterase|Yt blood group|acetylcholinesterase (Yt blood group)|apoptosis-related acetylcholinesterase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACHE, check out the ACHE Infographic

ACHE infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACHE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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2 Customer Q&As for Human ACHE ELISA Kit PicoKine®

Question

Is it possible to measure Butyrylcholinesterase (BChE) with this kit?

Verified customer

Asked: 2021-12-09

Answer

EK2137 Human ACHE ELISA Kit can not be used to measure Butyrylcholinesterase (BChE).

Boster Scientific Support

Answered: 2021-12-10

Question

If Q1 is no, do you have a kit that can measure Butyrylcholinesterase (BChE)?

Verified customer

Asked: 2021-12-09

Answer

Please use EK1507 Human BCHE ELISA Kit to measure Butyrylcholinesterase (BChE).

Boster Scientific Support

Answered: 2021-12-10

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