Human ADAM12 / MCMP ELISA Kit PicoKine™

Human ADAM12 / MCMP ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADAM12 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0934
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin) and urine.

Product Name

Human ADAM12 / MCMP ELISA Kit PicoKine™

See all ADAM12 products

SKU/Catalog Number

EK0934

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ADAM12 / MCMP ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADAM12 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human ADAM12 / MCMP ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0934)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: R29-S513

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0934 is reactive to ADAM12 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) and urine.

Application Guarantee

EK0934 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0934-CAP Anti-Human ADAM12 Pre-coated 96-well strip microplate 1
EK0934-ST Human ADAM12 Standard 2 vials, 10 ng/tube
EK0934-DA Human ADAM12 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0270.0840.1380.2640.4910.8311.3402.170

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect ADAM12, Dilution ratio of 1:10, concentration in serum is around 20ng/ml.

Some articles we found to cite concentrations of ADAM12 in samples: 22540429 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ADAM12 / MCMP ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2851490475525914984763
Standard deviation16.8175.4%9256.7719.6898.86338.17
CV (%)5.4%%7.1%%5.4%7.6%6.6%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ADAM12 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128530032128729814.344.8%
Sample 21490137615291374144268.654.7%
Sample 347554542463951464770229.534.8%
*number of samples for each test n=16.

Gene/Protein Information For ADAM12 (Source: Uniprot.Org, NCBI)

Uniprot ID

O43184

Gene ID

8038

Gene Name

ADAM12

Full Name

Disintegrin and metalloproteinase domain-containing protein 12

Weight

99.542kDa

Alternative Names

a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC 3.4.24.58; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.- ADAM12 ADAM12-OT1, CAR10, MCMP, MCMPMltna, MLTN, MLTNA ADAM metallopeptidase domain 12 disintegrin and metalloproteinase domain-containing protein 12|meltrin-alpha|metalloprotease-disintegrin 12 transmembrane

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADAM12, check out the ADAM12 Infographic

ADAM12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADAM12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human ADAM12 / MCMP ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-02-19

Question

Q: can I use citrate plasma as samples in Human ADAM12 Picokine® ELISA Kit (Catalog # EK0934)?

W. Garcia

Verified customer

Asked: 2020-01-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of ADAM12 causing degradation of its protein structure. ADAM12 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the ADAM12 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-01-18

Question

Q: What is the optimal O.D. value for ADAM12 ELISA kit? I performed your ADAM12 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ADAM12 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ADAM12 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ADAM12 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-11-15

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of ADAM12 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

S. Li

Verified customer

Asked: 2019-06-08

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ADAM12 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-06-08

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-09-29

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-09-29

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement ADAM12 using the EK0934 Human ADAM12 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2015-11-18

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2015-11-18

Question

Q: if the enzyme conjugated ADAM12 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ADAM12 antigen?

Verified Customer

Verified customer

Asked: 2015-09-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2015-09-16

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