Human Aggrecan/ACAN ELISA Kit PicoKine®

ACAN ELISA kit for Human

Human Aggrecan ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACAN in cell culture supernatants, serum and urine. Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK0909
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and urine.

Product Name

Human Aggrecan/ACAN ELISA Kit PicoKine®

View all ACAN ELISA kits

SKU/Catalog Number

EK0909

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Aggrecan ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACAN in cell culture supernatants, serum and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Aggrecan/ACAN ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0909)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: V20-R675

Sensitivity

<50 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0909 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0909 is reactive to ACAN in Human samples

Validated Sample Types

cell culture supernatants, serum and urine.

Application Guarantee

EK0909 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of ACAN

Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.

Kit Components

Catalog Number Description Quantity
EK0909-CAP Anti-Human ACAN Pre-coated 96-well strip microplate 1
EK0909-ST Human ACAN Standard 2 vials, 100 ng/tube
EK0909-DA Human ACAN Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.0010.0230.0520.1090.2350.5121.0331.887

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect ACAN, Dilution ratio of 1:1, concentration in serum and plasma is around 1.6 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Aggrecan/ACAN ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3084130644310233221398240208
Standard deviation240.55679.321939.59275.72908.831809.36
CV (%)7.8%5.2%4.5%8.3%6.5%4.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ACAN in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13084301131912954306088.562.8%
Sample 21306411683113381303612280779.416.3%
Sample 34310243347442894284743396544.91.2%
*number of samples for each test n=16.

Gene/Protein Information For ACAN (Source: Uniprot.Org, NCBI)

Gene Name

ACAN

Full Name

Aggrecan core protein

Weight

261.329kDa

Superfamily

aggrecan/versican proteoglycan family

Alternative Names

AGC1SEDK; aggrecan core protein; aggrecan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1aggrecan 1; CSPGCP; large aggregating proteoglycan; MSK16AGCAN ACAN AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, SSOAOD aggrecan aggrecan core protein|cartilage-specific proteoglycan core protein|chondroitin sulfate proteoglycan core protein 1|large aggregating proteoglycan

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACAN, check out the ACAN Infographic

ACAN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACAN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Human Aggrecan/ACAN ELISA Kit PicoKine®

Question

Q: Can ACAN ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

M. Collins

Verified customer

Asked: 2020-08-21

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-08-21

Question

Q: What is the optimal O.D. value for ACAN ELISA kit? I used your ACAN ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ACAN even though the O.D. values are not very high?

B. Singh

Verified customer

Asked: 2019-11-08

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ACAN ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of ACAN that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2019-11-08

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-07-22

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement ACAN using the EK0909 Human ACAN Picokine® ELISA Kit?

L. Garcia

Verified customer

Asked: 2019-04-18

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2019-04-18

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

K. Perez

Verified customer

Asked: 2018-03-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-03-18

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-02-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-02-09

Question

Q: which procedure should I follow in order to thaw whole blood sample for ACAN ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-01-19

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test ACAN for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-01-19

Question

Q: if the enzyme conjugated ACAN antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ACAN antigen?

Z. Nelson

Verified customer

Asked: 2015-06-23

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2015-06-23

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